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Summary
Genetic diversity and differentiation inindica andjaponica groups of the cultivated rice (Oryza sativa L.) were studied by assaying DNA restriction fragment length polymorphisms of 12indica and 14japonica rice lines digested with three restriction endonucleases. A total of 49 probes were selected to represent the entire RFLP map at intervals of 20–30 cM. It was shown that 95 of the 145 possible probe/enzyme combinations, involving 43 probes and all three enzymes, detected restriction fragment length variation, and the degree of polymorphism varied greatly from one probe/enzyme combination to another. These results demonstrate thatindica rice is genetically more diverse thanjaponica type. Significant differentiation between the two rice groups was detected by 33 probes representing 11 of the 12 rice chromosomes. It was deduced that the processes leading to differentiation involved a combination of molecular events that include base substitutions and insertion/deletions.
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Authors and Affiliations
Biotechnology Center, Huazhong Agricultural University, 430070, Wuhan, China
Qifa Zhang & B. Z. Shen
Department of Crop and Soil Environmental Sciences, Virginia Polytechnic Institute and State University, 24061, Blacksburg, VA, USA
M. A. Saghai Maroof & T. Y. Lu
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Communicated by Hu Han
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Zhang, Q., Maroof, M.A.S., Lu, T.Y.et al. Genetic diversity and differentiation ofindica andjaponica rice detected by RFLP analysis.Theoret. Appl. Genetics83, 495–499 (1992). https://doi.org/10.1007/BF00226539
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