Part of the book series:Methods in Molecular Biology™ ((MIMB,volume 501))
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Abstract
The determination of the concentration of infectious phage particles is fundamental to many protocols in phage biology, genetics, and molecular biology. Described here is a drop plaque assay, which, being simpler, faster and more efficient than either the classical overlay or direct plating methods, enhances efficiency in processing large numbers of samples.
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References
Arber, W. L., Enquist, L., Hohn, B., Murray, N. E., & Murray, K. (1983) inLambda II (Cold Spring Harbor Laboratory Press, Cold Spring Harbor Laboratory,NY), p. 433.
Sambrook, J. & Russell, D. W. (2001)Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Press, Cold Spring Harbor, New York).
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Authors and Affiliations
Public Health Agency of Canada, Laboratory for Foodborne Diseases, Guelph, Ontario, Canada
Amanda Mazzocco, Erika Lingohr & Roger P. Johnson
Pro-Lab Diagnostics, Richmond Hill, Ontario, Canada
Thomas E. Waddell
- Amanda Mazzocco
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- Thomas E. Waddell
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- Erika Lingohr
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- Roger P. Johnson
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Editors and Affiliations
University of Leicester, Leicester, UK
Martha R.J. Clokie
Public Health Agency of Canada, Guelph, Ontario, Canada
Andrew M. Kropinski
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Mazzocco, A., Waddell, T.E., Lingohr, E., Johnson, R.P. (2009). Enumeration of Bacteriophages Using the Small Drop Plaque Assay System. In: Clokie, M.R., Kropinski, A.M. (eds) Bacteriophages. Methods in Molecular Biology™, vol 501. Humana Press. https://doi.org/10.1007/978-1-60327-164-6_9
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