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Linco Research, Inc.

Premixed 16-Plex Mouse Cytokine LINCOplex Kit

All key components in this LINCOplex® kit are premixed and packaged in individual bottles for easy dispensation: conjugated beads, detection antibody, standards.

All components are included in each kit (capture antibody immobilized on microspheres, detection antibody, standards, quality controls, streptavidin-PE, wash buffer, assay buffer, 96-well microtiter filter plate, serum diluent) and are sufficient to run 39 samples (serum, plamsa or tissue culture medium) in duplicate in a 96-well microtiter plate.

ASSAY CONDITIONS:  
2 hour assay for tissue culture, overnight assay for serum/plasma, 25 µl sample volume.

STANDARD CURVE RANGE:  
3.2 - 10,000 pg/ml

SENSITIVITY IN TISSUE CULTURE:  
£ 3.2 - 16 pg/ml for all cytokines or better.

CROSS REACTIVITY:  
The cytokine antibody pairs in this multiplex do not cross react to the other analytes in the panel.  

 

CYTOKINES AVAILABLE

Serum
% Recovery

  
Mouse Cytokines: 
mIL-1b89.6
mIL-284.4
mIL-4110.5
mIL-582.2
mIL-681.3
mIL-985.9
mIL-1094.8
mIL-12p7086.2
mIL-1385.1
mTNF-a96.9
mIFN-g91.7
mGMCSF86.3
mMIP-1a94.8
mMCP-185.2
mKC85.1
mRANTES92.7

 

We will be expanding these cytokine panels as we go forward to include additional molecules important to your research needs.

This kit is for research only and not for use as a clinical or diagnostic tool.


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