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Constrained single particle tomography

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nextpyp/csp

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1. First, follow the instructions to build theETTK andcistem-1.0 packages using the intel compiler

2. Build cisTEM library (statically linked)

# Go to the cistem-1.0 repocd cistem-1.0# Use cistem container and enable intel compiler, for example:singularity shell -B /opt/apps cistem_dev_env_latest.sifsource  /opt/apps/rhel8/intel-2020/compilers_and_libraries/linux/bin/compilervars.sh intel64cd src./build_cspt_lib.sh# Exit the containerexit

(If the build was successful, you should see the filecspt_lib.a)

3. Build CSP

# Set up the path to the ettk & cistem buildsexport ETTK_PATH=path_to_your_ettk_projectexport CISTEM_PATH=path_to_your_cistem-1.0_project# Use ettk container for buildingsingularity shell ettk-devel.sif# Go to csp repo and link header file for cistem librarycd csp/src/ln -s ${CISTEM_PATH}/src/programs/refine3d_cspt/refine3d_cspt.h .rm CMakeCache.txt && rm -rf CMakeFilesccmake -D ITK_DIR=${ETTK_PATH}/ettk/external/InsightToolkit-4.2.1/build \       -D VTK_DIR=${ETTK_PATH}/ettk/external/VTK5.10.1/build        -D BLITZ_DIR=${ETTK_PATH}/ettk/external/blitz-0.9        -D CMAKE_CXX_COMPILER=/opt/apps/rhel8/intel-2020/compilers_and_libraries_2020.4.304/linux/bin/intel64/icpc        -D CMAKE_C_COMPILER=/opt/apps/rhel8/intel-2020/compilers_and_libraries_2020.4.304/linux/bin/intel64/icc        -D CMAKE_CXX_FLAGS="-no-multibyte-chars"        -D CMAKE_C_FLAGS="-no-multibyte-chars"       -D CISTEM_LIB=${CISTEM_PATH}/src/cspt_lib.a         .# The command will bring you to an interactive window, run the following in order(1) c(2) c(3) gmake csp

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