nextPYP is a comprehensive platform for single-particle cryo-EM/ET image analysis developed and maintained by theBartesaghi Lab atDuke University.

• Portable, easy-to-use, fully featured web-based GUI
• On-the-fly data pre-processing (single-particle and tomography)
• End-to-end pipeline for high-resolution structure determination (single-particle and tomography)
• 2D/3D particle picking using size-based, geometry-based and neural network-based approaches
• Scalable, small storage footprint (no need to save full size tomograms, sub-volumes, or particle stacks)
• Multi-mode constrained refinement and constrained classification for heterogeneity analysis
• Particle-based CTF refinement, movie frame refinement and self-tuning exposure weighting
• Import/export metadata in .star format to interface with external programs

See the fulldocumentation for more details.

Repositories