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The package provides a function that converts “.ace” files (ABI Sangercapillary sequence assembly files) to standard “.fastq” files. The fileformat is currently used in genomics to store contigs. To the best ofour knowledge, no R function is available to convert this format intothe more popular fastq file format. The development was motivated in thecontext of the analysis of 16S metagenomic data by the need to convertthe .ace files for further analysis.

You can install the released version of ace2fastq fromCRAN with:

install.packages("ace2fastq")

Latest version can be installed from github:

install.packages(devtools)devtools::install_github("c5sire/ace2fastq")

This is a basic example which shows you how to solve a common problem:

library(ace2fastq)filename<- system.file("sampledat/1.seq.ace",package="ace2fastq")out_file<- ace_to_fastq(filename,target_dir= tempdir())lines<- readLines(out_file$path)

#> [1] "@1.seq CO Contig1 1475 8 156 U"#> [1] "cgcttaaaccttccaagtctgaaacgggaaatttgatttgc"#> [1] "+"#> [1] "!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!"