Movatterモバイル変換

(Jinushi-Nakao et al., 2007)

abdominal anterior ventral multidendritic neuron vdaa

(Jinushi-Nakao et al., 2007)

(Jinushi-Nakao et al., 2007)

abdominal ventral multidendritic neuron vdaB

(Jinushi-Nakao et al., 2007)

first instar larval stage

(Adams et al., 1998)

(Adams et al., 1998)

(Adams et al., 1998)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

(Adams et al., 1998)

embryonic stage 17

(Adams et al., 1998)

(Adams et al., 1998)

(Adams et al., 1998)

(Adams et al., 1998)

(Adams et al., 1998)

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage --adult stage

(Darboux et al., 1998)

embryonic stage 17

(Darboux et al., 1998)

presumptive embryonic/larval peripheral nervous system |restricted

(Darboux et al., 1998)

(Darboux et al., 1998)

Additional Descriptive Data

ppk transcript is detected in embryonic, larval, and adult stages.ppk transcript and protein expression is detected in a subset of peripheral neurons in embryos starting at stage 17, and in early larvae. The subset of PNS neurons in which expression is detected are multidendritic neurons of the dendritic arborizing type. In abdominal segments A1-A7,ppk expression is detected in 1 of 6 dmd neurons, in the v'ada neuron, and in 1 of 5 vmd neurons. In thoracic segments,ppk expression is detected in 1 of 6 dmd neurons, and in 1 ofv'md neurons.

(Adams et al., 1998)

Low levels ofppk transcript expression are detected in early embryos using RT-PCR. Levels are higher in late embryos (14-16 hr), and expression is also detected in larvae, pupae, and adults. Using in situ hybridization, a segmentally repeated pattern of expression is detected in a subset of PNS cells during late embryogenesis. Two groups of neurons (dmd5 and v'md5) are stained in thoracic segments, and three groups (dmd6, v'ada, and vmd5) are stained in abdominal segments. The staining neurons belong to the dendritic arborizing subclas of multidentritic neurons.

(Darboux et al., 1998)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 17

(Adams et al., 1998)

(Adams et al., 1998)

first instar larval stage

(Adams et al., 1998)

larval peripheral nervous system |restricted

(Adams et al., 1998)

(Adams et al., 1998)

(Adams et al., 1998)

(Gorczyca et al., 2014)

Additional Descriptive Data

ppk transcript and protein expression is detected in a subset of peripheral nervous system neurons in embryos starting at stage 17, and in early larvae. The subset of PNS neurons in which expression is detected are multidendritic neurons of the dendritic arborizing type. In abdominal segments A1-A7,ppk expression is detected in 1 of 6 dmd neurons, in the v'ada neuron, and in 1 of 5 vmd neurons. In thoracic segments,ppk expression is detected in 1 of 5 dmd neurons, and in 1 of 5 v'md neurons.

(Adams et al., 1998)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_indendrite membrane

inferred from direct assay

(Gorczyca et al., 2014)

part_ofmonoatomic ion channel complex

inferred from direct assay

(Gorczyca et al., 2014)

Expression Deduced from Reporters

Reporter:P{ppk1-ppk1.EGFP}

Stage

Tissue/Position (including subcellular localization)

Reference

(Gorczyca et al., 2014)

Reporter:P{ppk.hs-mCD8.3xEGFP}

Stage

Tissue/Position (including subcellular localization)

Reference

prepupal stage --adult stage

(Shimono et al., 2009)

prepupal stage P1 --P3

(Shimono et al., 2009)

abdominal dorsal multidendritic neuron ddaD

(Shimono et al., 2009)

(Shimono et al., 2009)

larval abdominal lateral multidendritic neuron ldaA

(Shimono et al., 2009)

abdominal anterior ventral multidendritic neuron vdaa

(Shimono et al., 2009)

adult abdominal lateral multidendritic neuron ldaA-like

(Shimono et al., 2009)

Reporter:P{ppk-EGFP.1}

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 16 --late third instar larval stage

(Parrish et al., 2009)

embryonic stage 16 --P-stage

(Grueber et al., 2003)

larval multidendritic class IV neuron |subset

(Song et al., 2011)

abdominal anterior ventral multidendritic neuron vdaa

(Song et al., 2011)

(Song et al., 2011)

larval ventral multidendritic neuron vdaB

(Song et al., 2011)

neuron |subset oflateral oviduct

Comment:2 neurons

(Yang et al., 2009)

neuron |subset ofuterus

Comment:30 neurons

(Yang et al., 2009)

neuron |subset ofejaculatory duct

Comment:7 neurons

(Yang et al., 2009)

neuron |subset ofantenna

(Yang et al., 2009)

neuron |subset ofwing margin

(Yang et al., 2009)

neuron |subset ofleg

(Yang et al., 2009)

neuron |subset ofadult abdomen

Comment:body wall

(Yang et al., 2009)

adult sex peptide sensory neuron of uterus

(Rezaval et al., 2012)

neuron |subset oflateral oviduct

Comment:2 neurons

(Rezaval et al., 2012)

neuron |subset ofuterus

Comment:15 neurons

(Rezaval et al., 2012)

fruitless expressing neuron |subset ofuterus

(Häsemeyer et al., 2009)

sensory neuron |subset ofuterus

(Häsemeyer et al., 2009)

sensory neuron |subset ofoviduct

(Häsemeyer et al., 2009)

(Häsemeyer et al., 2009)

adult sex peptide sensory neuron of lateral oviduct

(Häsemeyer et al., 2009)

(Morikawa et al., 2011)

Reporter:P{ppk-GAL4.1.0}

Stage

Tissue/Position (including subcellular localization)

Reference

adult gnathal ganglion |restricted

(Häsemeyer et al., 2009)

(Häsemeyer et al., 2009)

fruitless expressing neuron |subset ofuterus

(Häsemeyer et al., 2009)

sensory neuron |subset ofuterus

(Häsemeyer et al., 2009)

sensory neuron |subset ofleg

(Häsemeyer et al., 2009)

adult sex peptide sensory neuron of uterus

(Häsemeyer et al., 2009)

(Häsemeyer et al., 2009)

Reporter:P{ppk-GAL4.1.9}

Stage

Tissue/Position (including subcellular localization)

Reference

(Williams et al., 2006)

central nervous system |restricted

(Ainsley et al., 2003)

(Ainsley et al., 2003)

(Eschbach et al., 2020,Tsubouchi et al., 2012,Hwang et al., 2007)

multidendritic neuron

(Thorne and Amrein, 2008)

abdominal dorsal multidendritic neuron ddaD

(Shimono et al., 2009)

(Shimono et al., 2009)

abdominal dorsal multidendritic neuron ddaE

(Shimono et al., 2009)

larval abdominal lateral multidendritic neuron ldaA

(Shimono et al., 2009)

abdominal anterior ventral multidendritic neuron vdaa

(Shimono et al., 2009)

adult abdominal lateral multidendritic neuron ldaA-like

(Shimono et al., 2009)

Reporter:P{ppk-GAL4.G}

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 16 --late third instar larval stage

(Parrish et al., 2009)

(Kim et al., 2012)

larval multidendritic class III neuron |faint

(Hu et al., 2017,Petersen and Stowers, 2011)

(Bloomington Drosophila Stock Center, 2017)

(Hattori et al., 2013)

adult brain |restricted

•neuron projection

(Yang et al., 2009)

dendritic arborizing neuron

•neuron projection

(Yang et al., 2009)

neuron |subset oflateral oviduct

Comment:2 neurons

(Yang et al., 2009)

neuron |subset ofuterus

Comment:30 neurons

(Yang et al., 2009)

(Yasunaga et al., 2010)

adult multidendritic neuron

(Zhang et al., 2020)

adult abdominal nerve trunk |restricted

sensory neuron |subset ofleg

(Häsemeyer et al., 2009)

sensory neuron |subset ofwing

(Häsemeyer et al., 2009)

sensory neuron |subset ofuterus

(Häsemeyer et al., 2009)

sensory neuron |subset ofoviduct

(Häsemeyer et al., 2009)

neuron |subset ofadult lateral horn

(Häsemeyer et al., 2009)

neuron |subset ofadult brain

(Häsemeyer et al., 2009)

fruitless expressing neuron |subset ofuterus

(Häsemeyer et al., 2009)

(Häsemeyer et al., 2009)

adult gnathal ganglion |restricted

(Häsemeyer et al., 2009)

(Häsemeyer et al., 2009)

mesothoracic nerve |restricted

(Häsemeyer et al., 2009)

adult cervical connective

(Häsemeyer et al., 2009)

adult sex peptide sensory neuron of uterus

(Häsemeyer et al., 2009)

adult sex peptide sensory neuron of lateral oviduct

(Häsemeyer et al., 2009)

(Gou et al., 2014)

Reporter:P{ppk-GAL4.H}

Stage

Tissue/Position (including subcellular localization)

Reference

(Han et al., 2012)

abdominal ventral multidendritic neuron vdaB

(Han et al., 2012)

(Han et al., 2012)

abdominal anterior ventral multidendritic neuron vdaa

(Han et al., 2012)

first instar larval stage --late third instar larval stage

(Soba et al., 2015)

Reporter:P{ppk-GAL4.U}

Stage

Tissue/Position (including subcellular localization)

Reference

(Song et al., 2015)

(Omamiuda-Ishikawa et al., 2020)

adult abdominal nerve to the uterus

(Liu et al., 2020)

Reporter:P{ppk-lexA.E}

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

(Luedke et al., 2024)

Reporter:P{ppk-lexA.G}

Stage

Tissue/Position (including subcellular localization)

Reference

(Gong et al., 2022)

(Gou et al., 2014)

Reporter:P{ppk-lexA-GAD}

Stage

Tissue/Position (including subcellular localization)

Reference

(Eschbach et al., 2020)

Reporter:P{ppk-Switch.R}

Stage

Tissue/Position (including subcellular localization)

Reference

(Lee et al., 2011)

adult abdominal anterior ventral multidendritic neuron vdaa

(Park et al., 2020)

adult abdominal dorsal multidendritic neuron ddaC

(Park et al., 2020)

(Gou et al., 2014)

Reporter:PBac{ppk-CD4-tdTom}

Stage

Tissue/Position (including subcellular localization)

Reference

(Han et al., 2012)

abdominal anterior ventral multidendritic neuron vdaa

(Han et al., 2012)

(Han et al., 2012)

abdominal ventral multidendritic neuron vdaB

(Han et al., 2012)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

ViewDmel\ppk in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage forDmel\ppk

View exonic expression by tissue forDmel\ppk

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

SeeGelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

ppk

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0020258

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34843/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0020258

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0020258

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0020258

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3478

Flygut - An atlas of the Drosophila adult midgut

FBgn0020258

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\ppk

Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 19 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 20 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of ppk

Transgenic constructs containing regulatory region of ppk

Aberrations (Deficiencies and Duplications) ( 7 )

Inferred from experimentation ( 7 )

Gene disrupted in

Df(2L)A400

(Ainsley et al., 2003)

Df(2L)b88h49

(Ainsley et al., 2003)

Df(2L)Exel6035

(Ryder, 2004.4.26)

Df(2L)k08712-rv21

(Roote, 2009.11.24)

Df(2L)ppk1Aid

(Wegman et al., 2010)

Df(2L)ppk1MirB

(Wegman et al., 2010)

Df(2L)ppk^ESB

(Boiko et al., 2012)

Inferred from location ( 7 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Other Phenotypes

Allele

abnormal behavior |larval stage, withDf(2L)ppk1Aid,Df(2L)ppk1MirB,Scer\GAL4^ppk.1.9

ppk^S551V.UAS

abnormal behavior |larval stage, withScer\GAL4^ppk.1.9

ppk^S551V.UAS

abnormal heat stress response |third instar larval stage, withScer\GAL4^ppk.1.9

abnormal locomotor behavior

ppk^RNAi.cAa

abnormal locomotor behavior, withScer\GAL4^ppk.1.9

ppk^E145X.UAS

abnormal locomotor behavior |larval stage, withScer\GAL4^109(2)80

ppk^RNAi.UAS

abnormal locomotor behavior |recessive |third instar larval stage

ppk^Δ16

abnormal locomotor behavior |third instar larval stage

ppk^ESB

abnormal locomotor behavior |third instar larval stage, withScer\GAL4^109(2)80

ppk^JF03250

abnormal locomotor behavior |third instar larval stage, withScer\GAL4^ppk.1.9

ppk^{deg.UAS.Tag:FLAG}

abnormal locomotor behavior |third instar larval stage, withScer\GAL4^ppk.PG

abnormal neuroanatomy |third instar larval stage, withScer\GAL4^ppk.1.9

ppk^KK104185

abnormal neurophysiology

ppk^ESB

abnormal neurophysiology, withScer\GAL4^ppk.1.9

ppk^{deg.UAS.Tag:FLAG}

abnormal pain response |third instar larval stage

abnormal pain response |third instar larval stage, withScer\GAL4^ppk26.PG

ppk^KK104185

abnormal pain response |third instar larval stage, withScer\GAL4^ppk.1.9

ppk^{deg.UAS.Tag:FLAG}

abnormal pain response |third instar larval stage, withScer\GAL4^ppk.PG

ppk^JF03250

abnormal stress response |larval stage

ppk^Δ16

abnormal touch response, withScer\GAL4^ppk.PG

ppk^KK104185

decreased body size, withScer\GAL4^ppk.1.9

ppk^S551V.UAS

increased body size, withDf(2L)Exel6035,Scer\GAL4^ppk.1.9

ppk^RNAi.cAa

Phenotype manifest in

Allele

dendrite |third instar larval stage, withScer\GAL4^ppk.1.9

ppk^KK104185

larval dorsal multidendritic neuron ddaC |third instar larval stage, withScer\GAL4^ppk.1.9

ppk^KK104185