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| Pronunciation | /ˈzaɪləziːn/ ZY-lə-zeen |
| Trade names | Rompun, Anased, Sedazine, Chanazine, others |
| AHFS/Drugs.com | International Drug Names |
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| Routes of administration | By mouth, inhalation, or injection (intravenous, intramuscular, or subcutaneous) |
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| ECHA InfoCard | 100.028.093 |
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| Formula | C12H16N2S |
| Molar mass | 220.33 g·mol−1 |
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Xylazine is astructural analog ofclonidine and anα2-adrenergic receptoragonist,[1] sold under many trade names worldwide, most notably theBayer brand nameRompun,[2] as well asAnased,Sedazine andChanazine.[3]
Xylazine is a common veterinary drug used forsedation,anesthesia,muscle relaxation, andanalgesia in animals such as horses, cattle, and other mammals.[2] Inveterinary anesthesia, it is often used in combination withketamine. Veterinarians also use xylazine as anemetic, especially in cats.[4]Drug interactions vary with different animals.[5][6][7]
Xylazine was first investigated for human use in the 1960s in West Germany forantihypertensive effects before being discontinued and marketed as a veterinary sedative. Xylazine mechanism of action was discovered in 1981, which led to the creation of otherα2-adrenergic receptor agonists such asmedetomidine anddexmedetomidine.
Xylazine has become a commonlyabusedstreet drug in the United States where it is known by the street name "tranq", particularly in the territory of Puerto Rico.[8] The drug is used as acutting agent forheroin andfentanyl.[9][10][11][12]
Xylazine was discovered as anantihypertensive agent in 1962 by Farbenfabriken Bayer inLeverkusen, West Germany.[13][14] In human trials xylazine was found to depress thecentral nervous system leading to the discontinuation of further research for its use in humans and it was instead marketed as a veterinary sedative. Xylazine was first used for this purpose in the late 1960s. Xylazine proved popular and in the 1970s became one of the most common large animal sedatives.[14] In 1981 a study discovered that the cause sedation was due to xylazine's effect on theα2-adrenergic receptor.[15][14] This led to the development of other α2-adrenergic receptor agonists such asdetomidine,medetomidine,dexmedetomidine, andromifidine.[14]
In the United States, xylazine was approved by the FDA only for veterinary use as asedative, analgesic, and muscle relaxant in dogs, cats, horses, elk,fallow deer,mule deer,sika deer, andwhite-tailed deer.[1][3] The sedative and analgesic effects of xylazine are related to central nervous system depression. Xylazine's muscle relaxant effect inhibits the transmission of neural impulses in the central nervous system.[16]
In scientific research usinganimal experiments, xylazine is a component of the most common anesthetic, ketamine-xylazine(see:Rodent cocktail), to anesthetize rats, mice, hamsters, and guinea pigs.[17]
Xylazine has not previously been a controlled substance; however, due to illicit abuse of xylazine legislative restrictions have been proposed in multiple countries.[14] Xylazine was made a class C drug in the UK on 15 January 2025.[18][19]
Xylazine is widely used in veterinary medicine as a sedative, muscle relaxant, and analgesic. It is frequently used in the treatment oftetanus.[1] It is not used in human medical treatment. Xylazine is similar to drugs such asphenothiazines,tricyclic antidepressants, and clonidine.[3] As an anesthetic, it is typically used in conjunction with ketamine.[20] In animals, xylazine may be administeredintramuscularly,intravenously, andintraosseously.[14][3]Subcutaneous,oral transmusocal andintranasal have been investigated but are not standard routes for xylazine administration.[14] As a veterinary anesthetic, xylazine is typically only administered once for the intended effect before or during surgical procedures.[1]α2-Adrenergic receptor antagonists such asatipamezole andyohimbine may be used to reverse the effects of xylazine in animals.[14][21][22][23] Xylazine is licensed for use in non-meat horses.Off-label use in cattle is common with recommended withholding periods of 1–5 days fordairy cattle and 4–10 days formeat cattle. Cattle are more sensitive to xylazine than horses with the sensitivity being greater in meat cattle breeds than dairy cattle breeds.[14]
Xylazine's use in cats and dogs is being replaced with the more selective alpha2 adrenergic receptor agonistsmedetomidine anddexmedetomidine and in some countries xylazine is rarely used with cats and dogs.[14]
High amounts ofcatecholamines in a patient will require higher doses of xylazine to be administered to provide sedation. The heightened levels required may not be practical or possible to administer and regular doses may cause excitement.[14]
Side effects in animals include transienthypertension andhypotension.[3] Xylazine decreases both respiration rate andminute ventilation, although the changes to PaCO2 and PaO2 are minor and innocuous.[14]
Xylazine has been demonstrated as reducing the dose ofepinephrine that causesarrythmia in dogs anaesthetised withisoflurane andhalothane.[14][24][25]
Xylazine administration in sheep activates pulmonary macrophages that damage thecapillary endothelium andalveolar type I cells. This in turns causes alveolar haemorrhage and oedema causing hypoxaemia.[14][26][27]
Intracarotid administration can cause seizures and excitement in horses.[14]
Xylazine has been shown to causemyometrial contractions in pregnant cattle.[28][14] Further evidence of xylazine's effect on pregnant animals is lacking and although other a2 adrenergic receptor agonists have been shown to not cause the same myometrial contraction the administration of a2 adrenergic receptor agonists is not recommended and should only be used in specific circumstances for animals near-term.[14]
Xylazine affects the glucose level via the activation of alpha2A andrenergic receptors onbeta cells, which prevents insulin release. alpha2 adrenergic receptors have been reported to cause transient hyperglycaemia with xylazine being reported as a cause in cattle and equine.[14][29][30] The renal threshold for glucose is not exceeded due to the hyperglycaemia with clinical doses. An alpha2 adrenergic receptor antagonist can reverse the effect.[14]
In dogs, sheep, horses, and cattle, thehalf-life is very short: only 1.21– 5.97 minutes. Complete elimination of the drug can take up to 23 minutes in sheep and up to 49 minutes in horses.[1][3] In young rats the half-life is one hour.[17] Xylazine has a largevolume of distribution ofVd =1.9 –2.5 for horses, cattle, sheep, and dogs.[3] Though the peakplasma concentrations are reached in12 –14 minutes in all species, thebioavailability varies between species.[3] The half-life depends on the age of the animal, as age is related to prolonged duration of anesthesia and recovery time.[17] Toxicity occurs with repeated administration, given that the metabolic clearance of the drug is usually calculated as 7– 9 times the half-life, which is 4 to 5 days for the clearance of xylazine.[17]
Xylazine is apotentα2-adrenergic receptor agonist.[32][3][14] When xylazine and otherα2-adrenergic receptor agonists are administered, they distribute throughout the body within 30 to 40 minutes.[16] Due to xylazine's highlylipophilic nature, it directly stimulates central α2-adrenergic receptors as well as peripheralα-adrenergic receptors in a variety of tissues.[1][3] As an agonist, xylazine reduces release ofnorepinephrine anddopamine in thecentral nervous system.[3] It does so by mimicking norepinephrine in binding to the pre-synaptic surfaceautoreceptors, which leads to feedback inhibition of norepinephrine release.[33]
Xylazine also serves as a transport inhibitor by suppressing norepinephrine transport function throughcompetitive inhibition of substrate transport. Accordingly, xylazine significantly increasesKm and does not affectVmax.[33] This likely occurs by direct interaction on an area that overlaps with the antidepressant binding site.[33] For example, xylazine andclonidine suppress uptake ofiobenguane (MIBG), a norepinephrine analogue, inneuroblastoma cells.[33] Xylazine's chemical structure closely resembles clonidine.
It has also been reported that xylazine activates theκ-opioid receptors, with low potency, which may contribute to its effects.[34]
Unlike other α2-adrenergic receptor agonists xylazine does not have anyimidazoline receptor activity. Xylazine binds at a ratio of 160:0, the lowest of all α2-adrenergic receptor agonists and 1/10th of that of medetomidine and dexmedotimidine.[14]
Xylazine is less selective than the other α2-Adrenergic receptor agonists.[14]
The analgesic effect of xylazine comes from binding to receptors at thesubstantia gelatinosa andlocus coeruleus.[14]
Xylazine is absorbed, metabolized, and eliminated rapidly. Xylazine can be inhaled or administered intravenously, intramuscularly, subcutaneously, or orally either by itself or in conjunction with other anesthetics, such as ketamine,barbiturates,chloral hydrate, andhalothane in order to provide reliable anesthesia effects.[12][20] The most common route of administration isinjection.[12]
Xylazine's action can be seen usually 15–30 minutes after administration and the sedative effect may continue for 1–2 hours and last up to 4 hours.[3] Once xylazine gains access to the vascular system, it is distributed within the blood, allowing xylazine to enter the heart, lungs, liver, and kidney.[35] In non-fatal cases, the blood plasma concentrations range from 0.03 to 4.6 mg/L.[3] Xylazine diffuses extensively and penetrates theblood–brain barrier, as might be expected due to the uncharged, lipophilic nature of the compound.[3]
Xylazine is metabolized by the liver'scytochrome P450 enzymes.[17] When it reaches the liver, xylazine is metabolized and proceeds to the kidneys to be excreted in urine.[36] Around 70% of a dose is excreted unchanged.[17] Thus, urine can be used in detecting xylazine administration because it contains manymetabolites, which are the main targets and products in urine.[1][37] Within a few hours, xylazine decreases to undetectable levels.[3] Other factors can also significantly impact the pharmacokinetics of xylazine, such as sex, nutrition, environmental conditions, and prior diseases.[17]
| Xylazine-M (2,6-dimethylaniline) | Xylazine-M (N-thiourea-2,6-dimethylaniline) | Xylazine-M (sulfone-HO-) isomer 2 |
| Xylazine-M (HO-2,6-dimethylaniline isomer 1) | Xylazine-M (HO-2,6-dimethylaniline isomer 2) | Xylazine M (oxo-) |
| Xylazine-M (HO-) isomer 1 | Xylazine-M (HO-) isomer 1 glucuronide | Xylazine-M (HO-) isomer 2 |
| Xylazine-M (HO-) isomer 2 glucuronide | Xylazine-M (HO-oxo-) isomer | Xylazine-M (HO-oxo-) isomer 1 glucuronide |
| Xylazine-M (HO-oxo-) isomer 2 | Xylazine-M (HO-oxo-) isomer 2 glucuronide | Xylazine-M (sulfone) |
| Xylazine-M (sulfone-HO-) isomer 1 |
In 1979, the first case of xylazine toxicity was reported in a 34-year-old male who had self-medicated for insomnia with an injection of 1g of xylazine.[38]
Xylazine is not regulated as a controlled substance under theControlled Substances Act. It is sold online through distributors often without requiring proof of a veterinary license. As a commonly used veterinary medicine xylazine is probably diverted from veterinary sources. The cost to purchase Xylazine from overseas suppliers is around $6–20 per kilogram. This low price makes it attractive for dealers looking for a cheap additive that is addictive and not treatable with opiate withdrawal medications.[39][40] The withdrawal can last for two weeks and has a quicker onset than fentanyl.[41]
Xylazine is most commonly ingested as an additive with fentanyl.[42] Xylazine has also been reported in combination withmedetomidine, another potentα2-adrenergic receptoragonist.[43] It is unknown if drug users are ingesting it knowingly. As of 2024, Seattle police report that some users wrongly believe they are consuming higher-quality fentanyl.[44][45][37] Xylazine's street name in Puerto Rico isanestesia de caballo, which translates to "horse anesthetic".[3][46] From 2002 to 2008, its use was associated with a high number of inmate deaths at theGuerrero Correctional Institution inAguadilla, Puerto Rico.[47]
Xylazine's street name in the United States, particularly when it is mixed withfentanyl, is "tranq", "tranq dope" and "zombie drug".[48]
As of 2012, xylazine users in Puerto Rico were more likely to be male, under age 30, living in a rural area, and injecting rather than inhaling xylazine. The combination of heroin and xylazine produces a potentially more deadlyhigh than administration of heroin alone. Xylazine is also frequently found in "speedball", a mixture of a stimulant drug such ascocaine with a depressant drug such asheroin,morphine and/orfentanyl.[12] As of 2012, causal factors underlying xylazine's increasing popularity were still unknown.[46]
As of 2022, more information on thedistribution of xylazine in the body, physical symptoms, and factors predictive of chronic use was known: when used, frequency of use depended on social or economic factors, as well as each user's subjective response to the drug's addictive properties.[49] From November 2021 until August 2022, 80% of drug paraphernalia which tested positive for fentanyl at needle exchange programs in Maryland also contained xylazine.[50] As of 2022, xylazine was almost invariably combined withopioids when used recreationally, and the drug produced a characteristicwithdrawal syndrome which complicates treatment of addicted users.[51][52]
In April 2023, theBiden administration declared xylazine-laced fentanyl an official emerging drug threat to the nation, the first time such a label has been given.[53] According to Gupta, xylazine is the deadliest drug threat the United States has ever faced. TheDrug Enforcement Administration (DEA) has seized xylazine and fentanyl mixtures in most states, finding 23% of seized fentanyl powder and 7% of fentanyl pills adulterated with xylazine.[8]
In July 2023, the first death following xylazine use outside of North America was reported to have taken place inSolihull, England in May 22. A 43-year-old male was found dead at home withpostmortemtoxicology detecting heroin, cocaine, fentanyl and xylazine.[54]
Xylazineoverdose is often fatal in humans.[1] Because it is used as a drugadulterant, the symptoms caused by the drugs accompanying xylazine administration vary between individuals.[12]
The most commonside-effects in humans associated with xylazine administration includebradycardia,respiratory depression,hypotension, transienthypertension secondary toα1-adrenergic receptor stimulation, and other central andhemodynamic changes.[1][12][55] Xylazine significantly decreasesheart rate in animals that are not pre-medicated with medications that haveanticholinergic effects.[1]
Other possible side-effects areareflexia,asthenia,ataxia,blurred vision,disorientation, dizziness, drowsiness,dysarthria,dysmetria, fainting,hyporeflexia,slurred speech,somnolence, staggering,coma,apnea, shallow breathing, sleepiness,premature ventricular contraction,tachycardia,miosis anddry mouth.[3] Rarely,hypotonia,urinary incontinence, and nonspecific electrocardiographicST segment changes occur.[3] Following a human overdose, symptoms can last for 8–72 hours, varying based on xylazine's combined usage with other drugs.[1][3]
Human tolerance to xylazine varies widely, with toxicity and fatality occurring between doses of 40–2,400 mg (0.62–37.04 gr).[3] Non-fatal blood or plasma concentration ranges from 0.03 to 4.6 mg/L.[35] In fatalities, the blood concentration of xylazine ranges from trace to 16 mg/L.[35] It is reported that there is no defined safe or fatal concentration of xylazine because of the significant overlap between the non-fatal and postmortem blood concentrations of xylazine.[3]
Hemodialysis has been suggested as a form of treatment, but is usually unfavorable due to the largevolume of distribution of xylazine.[3]
There are no standardizedscreenings to determine if an overdose has occurred. Detection of xylazine in humans involves various screening methods, such as urine screenings,thin layer chromatography (TLC),gas chromatography–mass spectrometry (GC-MS) andliquid chromatography–mass spectrometry (LC-MS).[37][35] As of November 2022, detecting xylazine in a drug sample requiresspectrophotometry.[56]
As of 1998[update], theα2-adrenergic receptorantagonistatipamezole was used to reverse the effects of xylazine or the related drugdexmedetomidine in veterinary medicine,[57] but this is not an approved medical treatment for humans, despitePhase I clinical trials in 2005.[58]
As of 2001,[update] the effects of xylazine in animals were also reversed by theanaleptics4-aminopyridine,doxapram, andcaffeine, which are physiological antagonists tocentral nervous system depressants.[59] The ways to accurately identify chronic xylazine usage are unknown, and the effective treatments, if any, are not standardized.[1][60] As of 2014,[update] multiple drugs have been used for therapeutic intervention, includinglidocaine,naloxone,thiamine,lorazepam,vecuronium,etomidate,propofol,tolazoline,yohimbine,atropine,orciprenaline,metoclopramide,ranitidine,metoprolol,enoxaparin,flucloxacillin,insulin, and irrigation of both eyes withsaline.[3]
The treatment after a xylazine overdose primarily involves maintaining respiratory function and blood pressure.[3] In cases of intoxication, physicians recommend intravenous fluid infusion, atropine, and hospital observation.[1] Severe cases may requiretracheal intubation,mechanical ventilation,gastric lavage,activated charcoal, bladdercatheterization,electrocardiographic (ECG) and hyperglycemia monitoring.[3] Physicians typically recommend which detoxification treatment should be used to manage possible dysfunction involving highlyperfused organs such as the liver and kidneys.[35]
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