 U.S. Highway 301 | ||||
|---|---|---|---|---|
 US 301 highlighted in red | ||||
| Route information | ||||
| Auxiliary route ofUS 1 | ||||
| Maintained byFDOT | ||||
| Length | 269.316 mi[1] (433.422 km) | |||
| Existed | 1949–present | |||
| Major junctions | ||||
| South end |  US 41 inSarasota | |||
| Major intersections | ||||
| North end |      US 1 /US 23 /US 301 /SR 4 /SR 15 south ofFolkston, Georgia | |||
| Location | ||||
| Country | United States | |||
| State | Florida | |||
| Counties | Sarasota,Manatee,Hillsborough,Pasco,Hernando,Sumter,Marion,Alachua,Bradford,Clay,Duval,Nassau | |||
| Highway system | ||||
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U.S. Route 301 (US 301) inFlorida runs from theSarasota-Bradenton-Venice, Florida Metropolitan Statistical Area northeast to theGreater Jacksonville Metropolitan Area. The road is a spur ofU.S. Route 1, which it intersects inCallahan.
US 301 carries the hidden state road designations ofState Road 683 fromSarasota to US 41 inBradenton,SR 55 from Bradenton toPalmetto,SR 43 fromUS 41 in Palmetto toUS 92 east ofTampa,SR 41 from US 92 toZephyrhills,SR 39 from Zephyrhills toUS 98 south ofDade City,State Road 35 from US 98 to Belleview (exceptSR 533 around downtown Dade City),SR 500 from Belleview toOcala,SR 200 from Ocala to Callahan andSR 15 from Callahan to the Georgia border, south ofFolkston, Georgia.
Concurrencies include U.S. 41 between Bradenton and Palmetto, U.S. 98 between Clinton Heights (near Dade City) and Trilacoochee (formerly known as Moss Town),US 27 between Belleview and Ocala,US 441 between Belleview and Sparr,US 90 in Baldwin, and U.S. 1 andUS 23 north of Callahan.
US 301 begins its northward journey at an intersection withU.S. Route 41 along Washington Boulevard in Sarasota. The first state highway it intersects with isSR 780. On the Sarasota-Manatee County line, it also intersects withCR 610, which takes commuters fromRingling College of Art and Design,University of South Florida'sSarasota/Manatee Campus,New College of Florida, andSarasota-Bradenton International Airport toInterstate 75. The next major intersection isSR 70, and after this, the road curves northwest before it eventually joins US 41(HiddenSR 55) again in southern Bradenton where they briefly overlap each other as they cross theManatee River.
US 301 makes a sharp right turn ontoSR 43 at a diamond interchange, then crossesI-75 in Ellenton at Exit 224. Just north of the intersection ofState Road 62, the road passes by theFlorida Gulf Coast Railroad Museum and then intersectsCR 683 (Moccasin Wallow Road). After the road crosses the Manatee-Hillsborough County Line, it passes by theLittle Manatee River State Park, then intersectsState Road 674, and shares a brief overlap withCR 672 between Balm Road and Big Bend Road.
North of the intersection with Gibsonton Drive, and over theAlafia River inRiverview, US 301 makes another turn to the northwest, where it crosses I-75 again nearBrandon at Exit 254. Beyond that, all access to I-75 is available from roads that cross US 301 in one way or another, the nearest of which being theLee Roy Selmon Expressway. Shortly before a diamond interchange with the Selmon Expressway, US 301 intersects withSR 676 at Causeway Boulevard, serving as the route's eastern terminus and providing access toUS 41 andUS Bus 41. The first major intersection north of the Selmon Expressway is withSR 60 at Adamo Drive.
At Six Mile Creek, an overpass takes US 301 over the tracks ofCSX's "A-line", one of therailroad company's main lines through Florida (also used byAmtrak'sSilver Star passenger trains), andCR 574, which is only accessible from local connecting roads. It then crosses the first bridge over theTampa Bypass Canal before intersectingSR 574 inEast Lake-Orient Park. At theFlorida State Fairgrounds, US 301 has a parclo interchange withInterstate 4 andU.S. Route 92 (SR 600). A reconstruction project took place in the early-2000s with provisions for a future ramp from westbound US 92, that are currently being obstructed by the Tampa Bypass Canal and land owned by theVandenberg Airport. Near the northern end of Vandenberg Airport, US 301 crosses over a flood channel that runs between the Hillsborough River and Tampa Bypass Canal on the southern border ofTemple Terrace.
Between Temple Terrace and Thonotosassa, the hidden SR 43 leaves US 301 and joinsCounty Road 580 at Harney Road, and the new hidden road becomesSR 41. Former sections of Harney Road are on hills on the northwest and northeast corners, both of which were designed to go around a former railroad bridge for a line that no longer exists. After crossing under I-75 with no access for the last time, and the Tampa Bypass Canal again shortly afterwards, US 301 encounters the eastern terminus ofSR 582 and its hidden County extension, County Road 582.
County Road 43 ends at US 301 in Thonotosassa along Harney Road, and US 301 loses more of its easterly trajection. Here, it serves as the northern terminus of Mango Road, and later Stacy Road, Sargeant Wilderness Memorial Park, and extension ofHillsborough River State Park. After passing by a field used for theTampa Machinery Auctions, US 301 officially runs along the eastern end of Hillsborough River State Park, which it will eventually enter. The same former railroad line that was encountered near Harney Road is visible along the east side of US 301 throughout the park and beyond. The last moderate intersection US 301 encounters before finally entering the park is the northern terminus of McIntosh Road. One residential area can be found on a minor side road off to the left, but the rest of the road is surrounded by parkland. US 301 leaves the park almost as instantly as it crosses thePasco County line.
After US 301 enters Pasco County, a slight curve to the right is a clear indication that 301 is enteringZephyrhills. Here, the road is known as Fort King Highway and Gall Boulevard depending on the location. Across from the fields of Festival Park, US 301 serves as the eastern terminus ofSR 56, and the first major intersection in Pasco County. The next intersection is with Chancey Road which eventually becomesCounty Road 535.SR 39 intersects with US 301 from the southeast. However, instead of terminating at Route 301, it becomes a hidden route north of the intersection and both roads head straight north again. This trajectory is short lived though when some local traffic is rerouted along 7th Street, and both roads intersect South Avenue before moving slightly northwest.
Downtown Zephyrhills is where US 301 serves as what would appear to be the eastern terminus ofSR 54, even though Fifth Avenue continues east of SR 54. Originally SR 54 extended east of US 301 along Fifth Avenue and then to 12th Street before turning back east again at Eiland Boulevard. In more recent times however, some maps have indicated that it serves as a third hidden route along US 301. Southbound motorists on US 301 have been encouraged to access SR 54 through 6th Street in recent years. After North Avenue, US 301 curves to the northeast as SR 41 leaves Route 301 to the northwest as County Road 41, along Fort King Road. The rest of 301 is known as Gall Boulevard until it moves deep into Dade City. After the curve to the northeast ends the road intersectsCounty Road 54, a county bypass and extension of SR 54 that leads toUS 98 (SR 700) on the Pasco-Polk County line. From there, US 301 runs along a series of steep hills as a four-lane divided highway with median openings that lack left-turn lanes until it reaches US 98 and the US 98-301 overlap begins. Immediately after this, the road intersectsSR 52 in Clinton Heights (nearDade City). While some of the median openings are still missing left-turn lanes, and is briefly replaced by a center left-turn lane, the road becomes much more level at this point.
In February 2007, US 98-301 was realigned to theformer truck route, which is two lanes wide as of this writing. Efforts to widen the road to four lanes has met stiff opposition from both the public and the city. The former truck route and old US 98-301, which is now SR 39 as well as hidden state routeshidden SRs 35–700, split from each other at an at-grade interchange. A local east–west street takes motorists to southboundCounty Road 35 Alternate. At the intersection of ALT CR 35 itself, the road moves from northeast back to northwest again, and runs along the CSX S-Line, where it encounters the eastern terminus ofCR 52 Alt and theDade City Atlantic Coast Line Railroad Depot.
US 98 & 301 reunite with the former route, near a former citrus plant. The first intersection after this is easternCounty Road 578 and the next one is at the northern border of the former citrus plant that leads to the Pioneer Florida Museum. As with the segment between Zephyrhills and Dade City, the road is a hilly four-lane divided highway with median openings that lack left-turn lanes, but the hills aren't as steep, and the road isn't as straight. After passing by the drive-in movie theater, US 98-301 intersection with Payne Road which was part of the former County Road 35B, There are some trailer parks, a golf course, and small motels in this segment. A sure sign the segment is about to end, is when it passed by the Owensboro Junction Trailhead, of theWithlacoochee State Trail. This was once a former junction between theAtlantic Coast Line andSeaboard Air Line Railroads, until both were merged into theSeaboard Coast Line and the segments that crossed Routes 98 & 301 were abandoned. US 98 leaves US 301 inTrilacoochee, veering to the northwest and taking hidden State Roads 39 and 700 with it. State Road 35 becomes the sole hidden state route as the road narrows down to two lanes before approachingState Road 575 and CR 575 inLacoochee. It remains a two-lane road even after it crosses the border withHernando County, Florida and over theWithlacoochee River.
In Hernando County, US 301 continues its two-lane rural surroundings until it approachesSR 50 inRidge Manor, which while hardly urban, contains some local businesses, and a large gas station, convenience store and car wash on the northeast corner. North of the Whispering Oaks Country Club, a gated community near SR 50, US 301 passes by random intersections of dirt roads, many of which are part of local deed-restricted communities.
US 301 entersSumter County by crossing over theLittle Withlacoochee River, where it shortly enters the unincorporated community ofCroom-a-Coochee, runs parallel to theCSX's S-Line, and intersects with County Road 675. InSt. Catherine, 301 intersects with CR 673, which leads to local campgrounds before reaching I-75, and almost instantly CR 478, which leads toWebster andCenter Hill.
InBushnell, US 301 becomes Main Street. The first main intersection there is County Road 476 (Seminole Avenue), a road that runs from northwestern Hernando County to north of Webster. CR 48 shares a hidden concurrency with US 301, that's not so hidden when it turns onto CR 476.Sumter County Road 48 runs north towards West Belt Avenue, taking the DeSoto Trail with it, while US 301 turns east on East Noble Avenue, and crosses CSX's S-Line, where it once shared a brief concurrency with CR 48 until that designation turned south onto Florida Avenue on its way to Lake County. From there, Route 301 shares an even shorter concurrency with County Road 476, until it curves back north.
US 301 passes by the ground ofLake-Sumter Community College's Sumter Campus inSumterville. Shortly after this, US 301 intersects County Road 470, and both roads share a concurrency. The north end ofSR 471 is encountered after this, and then the concurrency with County Road 470 breaks away to head east towardsOkahumpka. InColeman US 301 intersects with an abandoned railroad right-of-way that carried theSAL/AmtrakSilver Meteor until 1988. At this point, the road is briefly named South Commercial Street until it intersects with Warm Springs Avenue (County Road 514) and then heads east along Warm Springs Avenue. The road curves north again at the intersection of County Road 468.
Before US 301 entersWildwood, signs give advanced warnings of the road's approach to the next to last interchange forFlorida's Turnpike. Prior to this interchange, US 301 becomes a four-lane highway, which is sometimes divided. The next major intersection isSR 44, and both lead to I-75. Wildwood has also been referred to as "The Crossroads of Florida" due to this as well as the formerWildwood Amtrak Station, which now serves as a CSX maintenance yard. Until the 2010's US 301 narrowed back down to a two-lane highway with right-of-ways for future expansion on either sides north of the bridge. After enteringMarion County, it remains a divided highway as it intersectsCounty Road 42 inDallas, only to return to the two-lane road south of Wildwood. The stretch along the Marion-Sumter county line serves as the unofficial western boundary ofThe Villages, the large Central Florida retirement community.
North ofSummerfield, the road takes a slight curve to the northwest and then becomes a divided four-lane highway before curving back north again. The road remains no less than four lanes wide throughout the rest of Marion County, although just as in Pasco County, the median is missing left-turn lanes at many of the intersections.
WithinBelleview, the road curves more to the west and after the intersection withUS 27–441 (SR 25–500) is co-signed with these routes for a few blocks. However SR 35 turns right at the current eastern terminus ofCR 484, and US 27-301-441's new secret route becomesSR 25–500. After that the road curves more to the north again. Near the right of way for the formerly proposedCross Florida Barge Canal, the median for the road widens, but narrows back down to normal again. Before it does, however, it intersectsCR 328, where a police station exists between the median. Supports for a bridge that was intended to run over the canal exist behind the police station. To the west of this intersection is theSantos Trailhead of theCross Florida Greenway.[2]
Before US 27-301-441 entersOcala, it veers off to the left at an intersection with County Road Old 441 (Southeast Lake Weir Avenue), a former segment of US 441 that eventually leads toOcala Union Station. The first major intersection after this is 31st Street. Then it crosses under a railroad bridge before reaching the City Limits and the intersections with the northern terminus of CR 475 and crossingSR 464 (17th Street). US 27-301-441 intersects withSR 200 (becoming the new hidden state road until US 301 reaches US 1-23). The highway reaches the heart of Ocala at the intersection withSR 40 (West Silver Springs Boulevard). After this, the road crosses a railroad bridge west of Ocala Union Station. Five blocks later,US 27 leaves the US 301-441 overlap and takesSR 500 with it as they head northwest towardsWilliston,Perry,Tallahassee, and points north. Before US 301-441 leaves Ocala, it has an intersection withCounty Road 200A (Northwest 20th Street), which runs east and then north. This road was the former State Road 200A and decommissioned U.S. Route 301 Alternate.
Another county alternate of a state highway that the road intersects with is CR 25A (Northwest Gainesville Road), which runs northwest through towns such asZuber,Lowell, andReddick. East of Zuber, the road intersectsSR 326, which originally had a short concurrency, but now directly crosses US 301-441. South of the intersection withCR 329 is an interchange withU.S. Route 441 nearSparr, where the US 301-441 concurrency ends. US 441 moves northwest towardsGainesville, while US 301 moves northeast.
InCitra, US 301 intersects with the north end of CR 200A (Northeast Jacksonville Road), but another short former segment (Northeast 19th Terrace) appears on the opposite side before the road passes by the historicMelton-Shands House and intersects with CR 318. After US 301 crosses theAlachua County Line it runs along a four-lane causeway through marshland before enteringIsland Grove, where the road intersects with another former section (Southeast 201st Terrace) and CR 325, which leads toMarjorie Kinnan Rawlings Historic State Park. From there, the road makes a slight curve to the right to cross over the railroad line. Both the tracks and the road run along the east coast ofLochloosa Lake in Lochloosa. The interchange withSR 20 inHawthorne, also includes the side road Southeast 221st Street / Johnson Street, as well as the previously mentioned railroad line in between that side road and US 301. Intersections with CR 1474 in Campville andSR 26 inOrange Heights are at-grade interchanges, and the railroad line breaks away from the road moving straight north. US 301 would head back to the northwest inShenks, where it would otherwise take drivers straight into Lake Altho and those tracks can be found again, beneath the interchange withSR 24 inWaldo.
Since US 301 is a popular short cut between Northeastern Florida and the Gulf Coast region, a number of towns along the road have been notoriousspeed traps. The speed limit drops from 65 mph to 30 mph in a matter of a few hundred feet. Many have accused the police inWaldo,Starke,Lawtey, and others of giving tickets simply to raise money.[3][4] TheAmerican Automobile Association has strongly advised motorists to avoid this stretch of the road.[5]
North of Waldo, US 301 crossesBradford County Line. The first moderate intersection the road encounters is the southern terminus of County Road 221, which northbound traffic can access from a connecting ramp. East of Hampton Lake, it intersectsCR 18 betweenHampton and Hampton Beach. Upon entering Starke, US 301 gains the name Walnut Street; after it crosses a set of railroad tracks, it crosses over onto Temple Avenue. From there, the road intersectsSR 100 (Madison Street), and a block later the unmarkedSR 230 (Call Street), where it passes theOld Bradford County Courthouse, and serves as part of the boundary for theCall Street Historic District. The next major intersection in Starke isSR 16 (Brownlee Street).
A Starke Bypass was proposed for construction by theFDOT for the year 2010, and has been completed late summer of 2019[6][7][8] The interchanges that already exist with US 441 near Sparr, SR 20 in Hawthorne, and SR 24 in Waldo could also be used if the state were to upgrade the road as a freeway.
The next town over is Lawtey, which contains intersections with the eastern terminus of County Road 225 and then crosses County Road 125.
US 301 cuts through the northwest corner ofClay County with only one moderate intersection; County Road 218. After this, the road entersDuval County and the city ofJacksonville. As the road entersMaxville, it shares a brief concurrency withSR 228. After passing through Fiftone the road encounters a large railroad yard on the east side before the partial-cloverleaf interchange withI-10, before enteringBaldwin.
In September 2020, the Florida Department of Transportation opened the Baldwin Bypass around the western edge of town.[9]
The existing US 301 turns right at an intersection on the southern end of the new bypass, and then crosses railroad tracks that serve the suspendedAmtrakSunset Limited before making a right turn at a concurrency withU.S. 90 for several blocks. Immediately after another railroad crossing, both US 90 and US 301 narrow down to two lanes at the east end of this concurrency. US 301 remains a two-lane rural highway until it reaches the northern end of the Baldwin Bypass, and then turns right to continue north. The segment of US 301 between Baldwin andCallahan was originally two lanes, and was widened to 4 lanes in 2014.[10] US 301 then crosses intoNassau County, and passes through the small unincorporated towns ofBryceville, andCrawford. In Crawford, the highway crosses Norfolk Southern tracks via a bridge. US 301 then enters Callahan, where it crosses over a railroad bridge and gets the name West First Avenue. US 301 becomes East First Avenue at Booth Street before the intersection withUS 1/23. Here,SR A1A/200 continue running along East First Avenue towardFernandina Beach, while US 301 becomes part of a three route overlap with US 1-23 between Callahan, andFolkston, Georgia.
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US 301 first entered Florida in 1949, terminating in Tampa. It was rerouted along the 'old US 41' route to Palmetto in 1952, bypassing Tampa, and extended in 1953 south to Sarasota. In Sarasota, US 301 initially terminated at Main Street, which carried US 41 from Washington Boulevard to downtown Sarasota at the time.[11] By 1959, US 301 was extended a half mile south along Washington Boulevard in Sarasota when US 41 was rerouted to its current route along the newly-built Bayfront Drive along Sarasota Bay.[12]
From 1956 until 1993, US 301 signs in Florida featured black numbering on a yellow shield.[13] The "color-coding" of U.S. Routes by theFlorida Department of Transportation was stopped when the state could no longer use federal funds to replace signs that were not in compliance with theManual on Uniform Traffic Control Devices (MUTCD) per23 CFR655.603.[additional citation(s) needed] Signs replaced after this time period used the MUTCD standard of black numerals on a white shield surrounded by a rectangular black background. Some yellow US 301 signs may still remain.
Around 1985, U.S. 301 was realigned in southern Manatee County between the Sarasota County line and U.S. 41 to its current alignment along a formerAtlantic Coast Line Railroad corridor. U.S. 301 previously ran to the west along 15th Street and 301 Boulevard.[14][15]
| County | Location | mi[1] | km | Destinations | Notes | ||
|---|---|---|---|---|---|---|---|
| Sarasota | Sarasota | 0.000 | 0.000 | US 41 (Mound Street /Tamiami Trail /SR 45) | |||
| 0.722 | 1.162 |     SR 780 east (Fruitville Road) toI-75 | |||||
| Sarasota–Manatee county line | | 4.184 | 6.733 | ToI-75 / University Parkway (CR 610) –Sarasota-Bradenton International Airport,New College of Florida,USF Sarasota-Manatee | |||
| Manatee | | 8.244 | 13.267 |  SR 70 (53rd Avenue East / Oneco Road) toI-75 –Oneco,Arcadia | |||
| | 10.671 | 17.173 | SR 70 (15th Street East) | ||||
| Bradenton | 12.092 | 19.460 |  US 41 south (1st Street /SR 55) | interchange; southbound exit and northbound entrance; north end of SR 683 overlap; south end of US 41 / SR 55 overlap | |||
| 12.596 | 20.271 |  SR 64 east (6th Avenue) toI-75 –Zolfo Springs | |||||
| 12.686 | 20.416 |  SR 64 west (Manatee Avenue) –Anna Maria Island,Beaches | |||||
| | Hernando Desoto Bridge over theManatee River | ||||||
| Palmetto | 14.48[16] | 23.30 |    US 41 north (SR 55 north) toUS 19 north / 10th Street West (SR 43 south) –Manatee Fairgrounds,St. Petersburg | interchange; north end of US 41 / SR 55 overlap; south end of SR 43 overlap | |||
| Ellenton | 16.544 | 26.625 | Ellenton-Gillette Road (CR 683 north) | ||||
| | 18.02 | 29.00 | I-75 (SR 93) –Naples,Tampa,St. Petersburg | I-75 exit 224 | |||
| Parrish | 24.256 | 39.036 |   CR 675 east –Lake Manatee State Recreation Area,Arcadia | ||||
| 25.048 | 40.311 |  SR 62 east –Wauchula | |||||
| | 25.468 | 40.987 | ToI-75 / Moccasin Wallow Road (CR 683 west) | ||||
| Hillsborough | | 35.556 | 57.222 |  SR 674 (Sun City Center Boulevard) toI-75 –Sun City Center,Wimauma,Convention Center | |||
| | 39.520 | 63.601 |  CR 672 east –Balm | ||||
| | 41.036 | 66.041 |  CR 672 west (Big Bend Road) toI-75 | ||||
| Riverview | 45.558 | 73.318 | ToI-75 / Gibsonton Drive / Boyette Road | ||||
| | 49.11 | 79.03 |   I-75 (SR 93A) toSR 618 west (Selmon Expressway) /SR 60 east –Ocala,Brandon,Tampa,Naples | I-75 exit 254 | |||
| | 50.510 | 81.288 |  SR 676 west (Causeway Boulevard /CR 676 east) –Tampa,Port of Tampa,Brandon Town Center | ||||
| | 51.04 | 82.14 | SR 618 (Selmon Expressway) toI-75 –Ocala,Naples,Tampa,St. Petersburg | SR 618 exit 13 | |||
| | 52.375 | 84.289 | SR 60 (Adamo Drive) toI-75 /SR 618 (Selmon Expressway) –Brandon,Tampa | ||||
| | 53.73 | 86.47 | Overpass Road (to Broadway Avenue /CR 574) | interchange | |||
| East Lake-Orient Park | 54.681 | 88.001 |    SR 574 (Martin Luther King Jr. Boulevard) toI-4 west /I-75 –Mango,Tampa | ||||
| 55.291 | 88.982 | Elm Fair Boulevard –State Fairgrounds,Amphitheatre | north end of SR 43; south end of SR 41 | ||||
| 55.68 | 89.61 |  US 92 (SR 600) /I-4 toI-75 –Orlando,Tampa,St. Petersburg | I-4 exit 7 | ||||
| | 58.335 | 93.881 | Harney Road | ||||
| Thonotosassa | 60.237 | 96.942 |  SR 582 west (Fowler Avenue) toI-75 –Temple Terrace,University of South Florida,Busch Gardens | ||||
| | 62.395 | 100.415 |   CR 579 south (Mango Road) –Thonotosassa | ||||
| Pasco | |  SR 56 west –Wesley Chapel | Opened July 2019 | ||||
| | 74.799 | 120.377 | Chancey Road –Dade City | toCR 535, eastern bypass of Zephyrhills | |||
| | 75.496 | 121.499 |  SR 39 south –Crystal Springs,Plant City | north end of SR 41; south end of SR 39 overlap | |||
| Zephyrhills | 76.668 | 123.385 |  SR 54 west (5th Avenue) toI-75 –Land o' Lakes | ||||
| 77.472 | 124.679 | ToSR 54 west / 6th Street | |||||
| 77.822 | 125.242 |   CR 41 north (Fort King Road) | |||||
| 78.086 | 125.667 |   CR 54 (Eiland Boulevard) toI-75 /US 98 south | |||||
| | 83.074 | 133.695 | US 98 south (SR 35 /SR 700) –Lakeland | north end of SR 39; south end of US 98 / SR 35 / SR 700 overlap | |||
| Dade City | 83.369 | 134.169 |    SR 52 west /CR 52 Alt. east (Clinton Avenue) | ||||
| 85.208 | 137.129 | 7th Street (SR 35 north /SR 700 north) –Downtown | north end of SR 35 / SR 700 overlap; south end ofSR 533 | ||||
| 85.777 | 138.045 |  CR 35 Alt. south (Old Lakeland Highway) –Richland | |||||
| 86.059 | 138.499 | CR 52 west (Meridian Avenue) toI-75 –Downtown | |||||
| 86.810 | 139.707 | 7th Street (SR 35 south /SR 700 south) –Downtown | north end ofSR 533; south end of SR 35 / SR 700 overlap | ||||
| 86.999 | 140.011 | Lock Street –Spring Lake,Pasco-Hernando Community College | formerCR 578 west | ||||
| | 92.506 | 148.874 | US 98 north (SR 700) toI-75 –Brooksville,Perry | north end of US 98 / SR 700 overlap | |||
| Trilacoochee | 93.184 | 149.965 |   SR 575 north /CR 575 west (Trilby Road) –Trilby,Lacoochee | ||||
| Hernando | Ridge Manor | 96.317 | 155.007 |  SR 50 (Cortez Boulevard) toI-75 /US 98 north –Brooksville,Groveland | |||
| Sumter | St. Catherine | 103.768 | 166.998 |  CR 673 toI-75 | |||
| | 104.200 | 167.694 |  CR 478 east –Webster | ||||
| Bushnell | 107.211 | 172.539 |   CR 48 east /CR 476 west (Seminole Avenue) –Nobleton,Center Hill,Sumter County Fairgrounds | south end of CR 48 overlap | |||
| 107.744 | 173.397 |  CR 48 west /CR 475 north (North Main Street) toI-75 –Floral City | north end of CR 48 overlap | ||||
| 108.091 | 173.956 | CR 476 east | |||||
| Sumterville | 115.019 | 185.105 |  CR 470 west toI-75 –Lake Panasoffkee | south end of CR 470 overlap | |||
| | 115.342 | 185.625 |  SR 471 south –Webster,Sumter County Fairgrounds | ||||
| | 115.575 | 186.000 | CR 470 east –Correctional Facility | north end of CR 470 overlap | |||
| Wildwood | 119.968 | 193.070 |  CR 468 east | ||||
| 122.57 | 197.26 |   Florida's Turnpike (SR 91) toI-75 north | Turnpike exit 304 | ||||
| 123.297 | 198.427 |  SR 44 toI-75 –Inverness,Leesburg | |||||
| 124.089 | 199.702 |  CR 44A (Martin Luther King Jr. Avenue) | |||||
| 124.627 | 200.568 |  CR 466A east | |||||
| 125.873 | 202.573 |  CR 462 east | south end of CR 462 overlap | ||||
| | 126.132 | 202.990 | CR 462 west –Camp Wildwood[17] | north end of CR 462 overlap | |||
| Wildwood | 127.634 | 205.407 |  CR 472 east –Lake Miona Park | ||||
| Oxford | 128.898 | 207.441 |  CR 466 –The Villages | ||||
| Marion | Dallas | 132.681 | 213.529 |  CR 42 –Pedro,The Villages,Weirsdale | |||
| | 135.981 | 218.840 | Southeast 132nd Street Road | south bypass of Belleview | |||
| Belleview | 138.030 | 222.138 |   US 27 south /US 441 south (SR 500) | south end of US 27 / US 441 / SR 500 overlap | |||
| seeUS 441 (mile 308.734-330.372) | |||||||
| | 159.668 | 256.961 | US 441 north (SR 25) –Gainesville | north end of US 441 / SR 25 overlap | |||
| | 161.798 | 260.389 |  CR 316 –Reddick | ||||
| | 164.395 | 264.568 |  CR 200A south (Northeast Jacksonville Road) –Sparr,Anthony | ||||
| Citra | 165.615 | 266.532 |  CR 318 –Williston,Orange Springs,Horseshoe Lake Park | ||||
| Alachua | Island Grove | 168.124 | 270.569 |  CR 325 –Cross Creek,Marjorie Kinnan Rawlings State Historic Site | |||
| Hawthorne | 176.948 | 284.770 | CR 200A north (Southeast 221st Street) | ||||
| 177.888 | 286.283 |  CR 2082 west (Southeast 69th Avenue) –Downtown | |||||
| 178.30 | 286.95 |  SR 20 –Gainesville,Palatka | interchange | ||||
| | 180.167 | 289.951 |  CR 219A north | ||||
| Campville | 183.295 | 294.985 |  CR 1474 | ||||
| Orange Heights | 187.212 | 301.289 |  SR 26 –Gainesville,Melrose | interchange | |||
| Shenks | 190.404 | 306.426 |   CR 1469 south /CR 1471 north –Earleton | ||||
| Waldo | 192.798 | 310.278 |  ToSR 24 / Cole Street | ||||
| 193.0 | 310.6 | SR 24 west –Gainesville | interchange; no northbound exit | ||||
| | 196.087 | 315.571 |  CR 225 south | ||||
| Bradford | | 197.284 | 317.498 |  CR 221 north | |||
| Hampton | 198.263 | 319.073 |  CR 18 –Brooker | ||||
| | 200.367 | 322.459 | CR 221 south –Hampton | ||||
| | 201.032 | 323.530 |  CR 227 south –Brooker | ||||
| | 201.98 | 325.06 |  US 301 Alt. north (Starke Bypass /SR 223 north) | Southern terminus of US 301 Alt. and SR 223 | |||
| Starke | 203.435 | 327.397 |  CR 100A south (Southeast 44th Avenue) | toLincoln City | |||
| 204.136 | 328.525 | CR 100A west (Edwards Road) –National Guard Armory | |||||
| 204.518 | 329.140 |  SR 100 (Madison Street) –Lake City,Keystone Heights | |||||
| 204.570 | 329.224 |  SR 230 east (Call Street) | |||||
| 205.048 | 329.993 |  SR 16 (Dr. M.L. King Jr. Boulevard) –State Prison,Camp Blanding,Green Cove Springs | |||||
| 208.359 | 335.321 | US 301 Alt. south (Starke Bypass /SR 223 south) | Northern terminus of US 301 Alt. and SR 223 | ||||
| | 208.366 | 335.333 |  CR 233 | ||||
| | 209.840 | 337.705 | CR 200A north | ||||
| | 210.351 | 338.527 |  CR 200B north –Lawtey Correctional Institution | ||||
| Lawtey | 211.776 | 340.820 | CR 225 west (Madison Street) –State Prison | ||||
| 211.865 | 340.964 | CR 225 east /CR 200A south (Lake Street) –Camp Blanding,Lawtey Correctional Institution | |||||
| 212.701 | 342.309 |  CR 125 | |||||
| Clay | | 219.727 | 353.616 |  CR 218 east –Middleburg,Penney Farms | |||
| Duval | Maxville | 223.206 | 359.215 |  SR 228 east (Normandy Boulevard) –Jacksonville | south end of SR 228 overlap | ||
| 223.342 | 359.434 |  CR 228 west (Maxville Macclenny Highway) –Macclenny | north end of SR 228 overlap | ||||
| | 229.63 | 369.55 |  I-10 (SR 8) –Jacksonville,Lake City | ||||
 US 301 Byp. north (Baldwin Bypass /SR 201) | Truck Route of US 301 | ||||||
| Baldwin | 230.571 | 371.068 |  US 90 west (SR 10) | south end of US 90 / SR 10 overlap | |||
| 231.225 | 372.121 | US 90 east (SR 10) –Jacksonville | north end of US 90 / SR 10 overlap | ||||
| | US 301 Byp. south (Baldwin Bypass /SR 201) –Jacksonville,Starke | Truck Route of US 301 | |||||
| Nassau | | 236.691 | 380.917 |  CR 119 south (Otis Road) | |||
| Bryceville | 237.724 | 382.580 | CR 119 north | ||||
| Callahan | 251.425 | 404.629 | US 1 south /US 23 south (SR 15) –Jacksonville   SR A1A south /SR 200 east toI-95 –Yulee,Fernandina Beach | north end of SR 200 overlap; south end of US 1 / US 23 / SR 15 overlap | |||
| 251.642 | 404.979 | Brandies Avenue (CR 108 west) | |||||
| | 252.063 | 405.656 |  CR 115 north (Old Dixie Highway) | ||||
| Hilliard | 262.309 | 422.145 |  CR 108 | ||||
| Boulogne | 269.103 | 433.079 |  CR 121 south | ||||
| 269.316 | 433.422 | US 1 north /US 23 north /US 301 north /SR 4 north /SR 15 north –Folkston,Nahunta,Waycross | Georgia state line (St. Marys River bridge) | ||||
1.000 mi = 1.609 km; 1.000 km = 0.621 mi
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| U.S. Route 301 | ||
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