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This article is or was the subject of a Wiki Education Foundation-supported course assignment. Further details are availableon the course page. Student editor(s):Andrew.Tang88. Peer reviewers:Andrew.Tang88,Vicky.mai36.
Above undated message substituted fromTemplate:Dashboard.wikiedu.org assignment byPrimeBOT (talk)13:17, 16 January 2022 (UTC)[reply]
"Outside the field of analytical chemistry, the absorbance is sometimes defined as the natural logarithm instead of the base-10 logarithm." is what is said in the article, but I cannot figure out if that means the logarithm depicted is base 10 or the natural logarithm. Logically, I don't see how 10 could appear in the nature of light and absorbance, so I think that it is the natural logarithm. I am going to change it to LN, but if thats not right, somebody correct me.—Precedingunsigned comment added byEulerGamma (talk •contribs)
It's converted to Log because of the Beer-Lambert Law. The log is directly related to concentration. Also the absorbance is often reported as optical density or OD as the unit. The Log should be base 10 because that is the most common use. Clearly the choice of base is otherwise arbitrary.
I was wondering if a less technical aspect could be visualized with an photo like this? For the non-mathematical oriented reader. I am biased as I am the creator of the photo. Opinions? --Slaunger (talk)23:35, 2 December 2010 (UTC)[reply]
Anyone can multiply .79 by 100 and see that it is 79. Shouldn't we take out that useless third column (% Transmittance) from the table?—Precedingunsigned comment added by129.22.53.144 (talk)08:36, 18 April 2011 (UTC)[reply]
The two links for Color and Vision Research Laboratories are no longer valid. Can someone find current link on that site or from others that still verify this info? (Skoot13 (talk)15:34, 13 April 2012 (UTC))[reply]
In the very first line of the article "radiation falling upon a material, to the radiation transmitted through a material." there is a patently false statement. The reference is to "Webster's" but Webster's says (correctly) for physics "The ratio of the radiation absorbed by a surface to the total energy falling on that surface described as a percentage"
A definition of absorbance cannot be based only on incident and transmitted energy. This methode of definition would require reference to reflection (reflectance).
The full Webster's definition for absorbance is "A measure of the rate of decrease in the intensity of electromagnetic radiation (as light) as it passes through a given substance; the fraction of incident radiant energy absorbed per unit mass or thickness of an absorber"
Often in properly arranged measurements (typically using a cuvette as show in figure 1)and for nonscattering samples, reflection is ignored. However it is not possible to ignore it in a general definition. Take for example silver which typically has very low absorbance at any thickness even when %T is negligible.Bubsir (talk)14:24, 17 April 2012 (UTC)[reply]
As I understand it, this article was a result of a WikiProject. As there was some discussion of definition of absorbance, I led with definitions (there are two) from the "Gold Book". I added the Background sections because the mathematics centered on Optics, while the use of the term "absorbance" is probably most prominent in spectroscopy. I'm guessing that the word is used many times in Wikipedia in articles that I did not find. I hope someone will join in helping referencing this in the other articles.
I'm currently editing a college lab manual, and trying to determine whether the term "Optical Density" is suitable to describe absorbance (in the context of measuring DNA concentrations using a spectrophotometer, i.e., absorbance at a specific wavelength over a fixed distance). This page has been the only source I could find to suggest that it is not appropriate to use the term "Optical Density". However, it only says such use "is discouraged" without a source to who may be discouraging it, and why. Would it be appropriate to include further discussion on why it is discouraged or a source for such discouragement? Or would it be appropriate to remove the comment until a source can be found?
For context, I have foundthis page andthis page from manufacturers of plate readers that use the terms essentially interchangeablyThefezzident (talk)22:58, 28 July 2023 (UTC)[reply]