Protein-coding gene in the species Homo sapiens
FACT complex subunit SSRP1 also known asstructure specific recognition protein 1 is aprotein that in humans is encoded by the SSRP1gene .[ 5]
The protein encoded by this gene is a subunit of a heterodimer that, along withSUPT16H , forms chromatintranscriptional elongation factorFACT . FACT interacts specifically withhistones H2A /H2B to effect nucleosome disassembly and transcription elongation. FACT and cisplatin-damaged DNA may be crucial to the anticancer mechanism of cisplatin. This encoded protein contains ahigh mobility group box which most likely constitutes the structure recognition element for cisplatin-modified DNA. This protein also functions as a co-activator of the transcriptional activator p63.[ 5]
Structure specific recognition protein 1 has been shown tointeract withNEK9 .[ 6] p63 .[ 7]
^a b c GRCh38: Ensembl release 89: ENSG00000149136 –Ensembl , May 2017^a b c GRCm38: Ensembl release 89: ENSMUSG00000027067 –Ensembl , May 2017^ "Human PubMed Reference:" .National Center for Biotechnology Information, U.S. National Library of Medicine .^ "Mouse PubMed Reference:" .National Center for Biotechnology Information, U.S. National Library of Medicine .^a b "Entrez Gene: SSRP1 structure specific recognition protein 1" .^ Tan BC, Lee SC (Mar 2004)."Nek9, a novel FACT-associated protein, modulates interphase progression" .J. Biol. Chem .279 (10):9321– 30.doi :10.1074/jbc.M311477200 PMID 14660563 . ^ Zeng SX, Dai MS, Keller DM, Lu H (15 Oct 2002)."SSRP1 functions as a co-activator of the transcriptional activator p63" .EMBO J .21 (20):5487– 97.doi :10.1093/emboj/cdf540 .PMC 129072 PMID 12374749 . Bruhn SL, Pil PM, Essigmann JM, Housman DE, Lippard SJ (1992)."Isolation and characterization of human cDNA clones encoding a high mobility group box protein that recognizes structural distortions to DNA caused by binding of the anticancer agent cisplatin" .Proc. Natl. Acad. Sci. U.S.A .89 (6):2307– 11.Bibcode :1992PNAS...89.2307B .doi :10.1073/pnas.89.6.2307 PMC 48646 PMID 1372440 . Toney JH, Donahue BA, Kellett PJ, Bruhn SL, Essigmann JM, Lippard SJ (1989)."Isolation of cDNAs encoding a human protein that binds selectively to DNA modified by the anticancer drug cis-diamminedichloroplatinum(II)" .Proc. Natl. Acad. Sci. U.S.A .86 (21):8328– 32.Bibcode :1989PNAS...86.8328T .doi :10.1073/pnas.86.21.8328 PMC 298274 PMID 2530581 . Nagulapalli S, Pongubala JM, Atchison ML (1995)."Multiple proteins physically interact with PU.1. Transcriptional synergy with NF-IL6 beta (C/EBP delta, CRP3)" .J. Immunol .155 (9):4330– 8.doi :10.4049/jimmunol.155.9.4330 PMID 7594592 . Orphanides G, LeRoy G, Chang CH, Luse DS, Reinberg D (1998)."FACT, a factor that facilitates transcript elongation through nucleosomes" .Cell .92 (1):105– 16.doi :10.1016/S0092-8674(00)80903-4 PMID 9489704 .S2CID 11248520 . Dyer MA, Hayes PJ, Baron MH (1998)."The HMG domain protein SSRP1/PREIIBF is involved in activation of the human embryonic beta-like globin gene" .Mol. Cell. Biol .18 (5):2617– 28.doi :10.1128/MCB.18.5.2617 .PMC 110641 PMID 9566881 . LeRoy G, Orphanides G, Lane WS, Reinberg D (1998). "Requirement of RSF and FACT for transcription of chromatin templates in vitro".Science .282 (5395):1900– 4.doi :10.1126/science.282.5395.1900 .PMID 9836642 . Kelley DE, Stokes DG, Perry RP (1999). "CHD1 interacts with SSRP1 and depends on both its chromodomain and its ATPase/helicase-like domain for proper association with chromatin".Chromosoma .108 (1):10– 25.doi :10.1007/s004120050347 .PMID 10199952 .S2CID 12945778 . Spencer JA, Baron MH, Olson EN (1999)."Cooperative transcriptional activation by serum response factor and the high mobility group protein SSRP1" .J. Biol. Chem .274 (22):15686– 93.doi :10.1074/jbc.274.22.15686 PMID 10336466 . Orphanides G, Wu WH, Lane WS, Hampsey M, Reinberg D (1999). "The chromatin-specific transcription elongation factor FACT comprises human SPT16 and SSRP1 proteins".Nature .400 (6741):284– 8.Bibcode :1999Natur.400..284O .doi :10.1038/22350 .PMID 10421373 .S2CID 4300397 . Wada T, Orphanides G, Hasegawa J, Kim DK, Shima D, Yamaguchi Y, Fukuda A, Hisatake K, Oh S, Reinberg D, Handa H (2000)."FACT relieves DSIF/NELF-mediated inhibition of transcriptional elongation and reveals functional differences between P-TEFb and TFIIH" .Mol. Cell .5 (6):1067– 72.doi :10.1016/S1097-2765(00)80272-5 PMID 10912001 . Keller DM, Zeng X, Wang Y, Zhang QH, Kapoor M, Shu H, Goodman R, Lozano G, Zhao Y, Lu H (2001)."A DNA damage-induced p53 serine 392 kinase complex contains CK2, hSpt16, and SSRP1" .Mol. Cell .7 (2):283– 92.doi :10.1016/S1097-2765(01)00176-9 PMID 11239457 . Yarnell AT, Oh S, Reinberg D, Lippard SJ (2001)."Interaction of FACT, SSRP1, and the high mobility group (HMG) domain of SSRP1 with DNA damaged by the anticancer drug cisplatin" .J. Biol. Chem .276 (28):25736– 41.doi :10.1074/jbc.M101208200 PMID 11344167 . Santoro P, De Andrea M, Migliaretti G, Trapani C, Landolfo S, Gariglio M (2002). "High prevalence of autoantibodies against the nuclear high mobility group (HMG) protein SSRP1 in sera from patients with systemic lupus erythematosus, but not other rheumatic diseases".J. Rheumatol .29 (1):90– 3.PMID 11824977 . Roig J, Mikhailov A, Belham C, Avruch J (2002)."Nercc1, a mammalian NIMA-family kinase, binds the Ran GTPase and regulates mitotic progression" .Genes Dev .16 (13):1640– 58.doi :10.1101/gad.972202 .PMC 186374 PMID 12101123 . Zeng SX, Dai MS, Keller DM, Lu H (2002)."SSRP1 functions as a co-activator of the transcriptional activator p63" .EMBO J .21 (20):5487– 97.doi :10.1093/emboj/cdf540 .PMC 129072 PMID 12374749 . Keller DM, Lu H (2003)."p53 serine 392 phosphorylation increases after UV through induction of the assembly of the CK2.hSPT16.SSRP1 complex" .J. Biol. Chem .277 (51):50206– 13.doi :10.1074/jbc.M209820200 PMID 12393879 . Belotserkovskaya R, Oh S, Bondarenko VA, Orphanides G, Studitsky VM, Reinberg D (2003). "FACT facilitates transcription-dependent nucleosome alteration".Science .301 (5636):1090– 3.Bibcode :2003Sci...301.1090B .doi :10.1126/science.1085703 .PMID 12934006 .S2CID 26667338 . Li J, Hawkins IC, Harvey CD, Jennings JL, Link AJ, Patton JG (2003)."Regulation of alternative splicing by SRrp86 and its interacting proteins" .Mol. Cell. Biol .23 (21):7437– 47.doi :10.1128/MCB.23.21.7437-7447.2003 .PMC 207616 PMID 14559993 . Tan BC, Lee SC (2004)."Nek9, a novel FACT-associated protein, modulates interphase progression" .J. Biol. Chem .279 (10):9321– 30.doi :10.1074/jbc.M311477200 PMID 14660563 . This article incorporates text from theUnited States National Library of Medicine , which is in thepublic domain .
(1) Basic domains
(1.1) Basic leucine zipper (bZIP )(1.2) Basic helix-loop-helix (bHLH )
Group A Group B Group CPAS Group D Group E Group F
(1.3) bHLH-ZIP (1.4) NF-1 (1.5) RF-X (1.6) Basic helix-span-helix (bHSH)
(2.1) Nuclear receptor (Cys4 )
subfamily 1 subfamily 2 subfamily 3 subfamily 4 subfamily 5 subfamily 6 subfamily 0
(2.2) Other Cys4 (2.3) Cys2 His2 (2.4) Cys6 (2.5) Alternating composition (2.6) WRKY
(4)β-Scaffold factors with minor groove contacts
(0) Other transcription factors
