Crystallographic structure of the PAS domain of the bacterial oxygen sensor protein fixL.[1] The protein is depicted as a rainbow colored cartoon (N-terminus = blue,C-terminus = red) while theheme ligand is shown as sticks (carbon = white, nitrogen = blue, oxygen = red, iron = orange).
APer-Arnt-Sim (PAS)domain is aprotein domain found in all kingdoms of life.[2] Generally, the PAS domain acts as a molecular sensor, whereby small molecules and other proteins associate via binding of the PAS domain.[3][4][5] Due to this sensing capability, the PAS domain has been shown as the keystructural motif involved in protein-protein interactions of thecircadian clock, and it is also a common motif found in signaling proteins, where it functions as a signaling sensor.[6][7]
PAS domains are found in a large number of organisms from bacteria to mammals. The PAS domain was named after the three proteins in which it was first discovered:[8]
Since the initial discovery of the PAS domain, a large quantity of PAS domain binding sites have been discovered in bacteria and eukaryotes. A subset calledPAS LOV proteins are responsive to oxygen, light and voltage.[9]
Although the PAS domain exhibits a degree of sequence variability, the three-dimensional structure of the PAS domain core is broadly conserved.[10] This core consists of a five-stranded antiparallel β-sheet and several α-helices. Structural changes, as a result of signaling, predominantly originate within theβ-sheet. These signals propagate via theα-helices of the core to the covalently-attached effector domain.[11] In 1998, the PAS domain core architecture was first characterized in the structure ofphotoactive yellow protein (PYP) fromHalorhodospira halophila.[10] In many proteins, a dimer of PAS domains is required, whereby one binds a ligand and the other mediates interactions with other proteins.[5]
The PAS domains that are known share less than 20% averagepairwise sequence identity, meaning they are surprisingly dissimilar.[10] PAS domains are frequently found on proteins with other environmental sensing mechanisms. Also, many PAS domains are attached to photoreceptive cells.[12]
In the presence of light,White Collar-1 (WC-1) and White Collar-2 (WC-2) dimerizes via mediation by the PAS domains, which activates translation ofFRQ.[13]
In the presence of light,CLK andCYC attach via a PAS domain, activating the translation of PER, which then associates toTim via the PER PAS domain. The following genes contain PAS binding domains: PER, Tim, CLK, CYC.
A PAS domain is found in the ZTL and NPH1 genes. These domains are very similar to the PAS domain found in theNeurospora circadian-associated protein WC-1.[14]
The circadian clock that is currently understood for mammals begins when light activatesBMAL1 andCLK to bind via their PAS domains. That activator complex regulates Per1, Per2, and Per3 which all have PAS domains that are used to bind to cryptochromes 1 and 2 (CRY 1,2 family). The following mammalian genes contain PAS binding domains: Per1, Per2, Per3, Cry1, Cry2, Bmal, Clk, Pasd1.
Within Mammals, both PAS domains play important roles. PAS A is responsible for the protein-protein interactions with other PAS domain proteins, while PAS B has a more versatile role. It mediates interactions withchaperonins and other small molecules likedioxin, but PAS B domains inNPAS2, a homolog of the Drosophila clk gene, and thehypoxia inducible factor (HIF) also help to mediateligand binding.[12] Furthermore, PAS domains containing the NPAS2 protein have been shown to be a substitute for the Clock gene in mutant mice who lack the Clock gene completely.[15]
The PAS domain also directly interacts withBHLH. It is typically located on theC-Terminus of the BHLH protein. PAS domains containing BHLH proteins form a BHLH-Pas protein, typically found and encoded in HIF, which require both the PAS domain and BHLH domain and the Clock gene.[16][17][18]
These cGMP-binding domains are found in diverse phototransducing proteins acrosseukaryotes andeubacteria. They are present in plant and cyanobacterialphytochromes, vertebrate and invertebrate cGMP-stimulatedphosphodiesterases (PDEs) and some non-photosynthetic eubacteria.[19][20][21]
These extracellular signaling domains are homologous to PAS domains but distinct.[22] They are common to animalcalcium (Ca2+) channel subunits and certainprokaryoticchemotaxis receptors and play a role in small-molecule recognition across various species, suggesting a conserved mechanism of ligand binding.[23] As opposite to the intracellular PAS and GAF domains, they show a long extra N-terminalalpha helix.[22]
^Harmer SL, Panda S, Kay SA (28 November 2003). "Molecular bases of circadian rhythms".Annual Review of Cell and Developmental Biology.17:215–253.doi:10.1146/annurev.cellbio.17.1.215.PMID11687489.
^abGalperin MY, Nikolskaya AN, Koonin EV (September 2001). "Novel domains of the prokaryotic two-component signal transduction systems".FEMS Microbiology Letters.203 (1):11–21.doi:10.1016/S0378-1097(01)00326-3.PMID11557134.
^Aravind L, Ponting CP (December 1997). "The GAF domain: an evolutionary link between diverse phototransducing proteins".Trends in Biochemical Sciences.22 (12):458–459.doi:10.1016/s0968-0004(97)01148-1.PMID9433123.
^Anantharaman V, Aravind L (November 2000). "Cache - a signaling domain common to animal Ca(2+)-channel subunits and a class of prokaryotic chemotaxis receptors".Trends in Biochemical Sciences.25 (11):535–537.doi:10.1016/S0968-0004(00)01672-8.PMID11084361.
