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| Names | |
|---|---|
| Other names Acid Orange 10 C.I. 16230 | |
| Identifiers | |
3D model (JSmol) | |
| ChEBI | |
| ChEMBL | |
| ChemSpider |
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| ECHA InfoCard | 100.016.096 |
| KEGG |
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| UNII | |
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| Properties | |
| C16H10N2Na2O7S2 | |
| Molar mass | 452.38 g/mol |
| Melting point | 141 °C |
| Hazards | |
| Occupational safety and health (OHS/OSH): | |
Main hazards | R36/37/38, S26, S36 |
Except where otherwise noted, data are given for materials in theirstandard state (at 25 °C [77 °F], 100 kPa). | |
Orange G also calledC.I. 16230,[1]Acid Orange 10,[1] ororange gelb[2] is a syntheticazo dye used inhistology in manystaining formulations. It usually comes as a disodium salt. It has the appearance oforange crystals or powder.
Orange G is used in thePapanicolaou stain[3] to stainkeratin. It is also a major component of the Alexander test forpollen staining.
It is often combined with other yellow dyes and used to stainerythrocytes in thetrichrome methods.
Orange G can be used as anelectrophoretic color marker to monitor the process ofagarose gel electrophoresis, running approximately at the size of a 50Base pair (bp) DNA molecule, andpolyacrylamide gel electrophoresis.Bromophenol blue andxylene cyanol can also be used for this purpose. (However, the apparent "size" of all these dyes varies according to the concentration of agarose in the gel and the buffer system used, so one should look up the appropriate reference before using the dyes to determine how far a gel has run.)
Despite its three ionizable groups, it shows only two colors in aqueous solution, brilliant orange in neutral and acidicpH or red in pH greater than 9.
