In cellular metabolism, NAD is involved in redox reactions, carryingelectrons from one reaction to another, so it is found in two forms: NAD+ is anoxidizing agent, accepting electrons from other molecules and becoming reduced; with H+, this reaction forms NADH, which can be used as areducing agent to donate electrons. Theseelectron transfer reactions are the main function of NAD. It is also used in other cellular processes, most notably as asubstrate ofenzymes in adding or removingchemical groups to or fromproteins, inposttranslational modifications. Because of the importance of these functions, the enzymes involved in NAD metabolism are targets fordrug discovery.
In organisms, NAD can besynthesized from simple building-blocks (de novo) from eithertryptophan oraspartic acid, each a case of anamino acid. Alternatively, more complex components of the coenzymes are taken up from nutritive compounds such asnicotinic acid; similar compounds are produced by reactions that break down the structure of NAD, providing asalvage pathway that recycles them back into their respective active form.
In the name NAD+, thesuperscripted plus sign indicates the positiveformal charge on one of its nitrogen atoms.A biological coenzyme that acts as an electron carrier in enzymatic reactions.
Some NAD is converted into the coenzymenicotinamide adenine dinucleotide phosphate (NADP), whose chemistry largely parallels that of NAD, though its predominant role is as a coenzyme inanabolic metabolism.NADP is a reducing agent in anabolic reactions like the Calvin cycle and lipid and nucleic acid syntheses. NADP exists in two forms: NADP+, the oxidized form, and NADPH, the reduced form. NADP is similar to nicotinamide adenine dinucleotide (NAD), but NADP has a phosphate group at the C-2′ position of the adenosyl.
Theredox reactions of nicotinamide adenine dinucleotide
The compound accepts or donates the equivalent of H−.[4] Such reactions (summarized in formula below) involve the removal of two hydrogen atoms from a reactant (R), in the form of ahydride ion (H−), and aproton (H+). The proton is released into solution, while the reductant RH2 is oxidized and NAD+ reduced to NADH by transfer of the hydride to the nicotinamide ring.
RH2 + NAD+ → NADH + H+ + R;
From the electron pair of the hydride ion, one electron is attracted to the slightly more electronegative atom of the nicotinamide ring of NAD+,[5] becoming part of the nicotinamide moiety. The remaining hydrogen atom is transferred to the carbon atom opposite the N atom. Themidpoint potential of the NAD+/NADH redox pair is −0.32 volts, which makes NADH a moderately strongreducing agent.[6] The reaction is easily reversible, when NADH reduces another molecule and is re-oxidized to NAD+. This means the coenzyme can continuously cycle between the NAD+ and NADH forms without being consumed.[3]
In appearance, all forms of this coenzyme are whiteamorphous powders that arehygroscopic and highly water-soluble.[7] The solids are stable if stored dry and in the dark. Solutions of NAD+ are colorless and stable for about a week at 4 °C and neutralpH, but decompose rapidly in acidic or alkaline solutions. Upon decomposition, they form products that areenzyme inhibitors.[8]
Both NAD+ and NADH strongly absorbultraviolet light because of the adenine. For example, peak absorption of NAD+ is at awavelength of 259 nanometers (nm), with anextinction coefficient of 16,900 M−1cm−1. NADH also absorbs at higher wavelengths, with a second peak in UV absorption at 339 nm with an extinction coefficient of 6,220 M−1cm−1.[9] This difference in the ultravioletabsorption spectra between the oxidized and reduced forms of the coenzymes at higher wavelengths makes it simple to measure the conversion of one to another inenzyme assays – by measuring the amount of UV absorption at 340 nm using aspectrophotometer.[9]
NAD+ and NADH also differ in theirfluorescence. Freely diffusing NADH in aqueous solution, when excited at the nicotinamide absorbance of ~335 nm (near-UV), fluoresces at 445–460 nm (violet to blue) with afluorescence lifetime of 0.4 nanoseconds, while NAD+ does not fluoresce.[10][11] The properties of the fluorescence signal changes when NADH binds toproteins, so these changes can be used to measuredissociation constants, which are useful in the study ofenzyme kinetics.[11][12] These changes in fluorescence are also used to measure changes in the redox state of living cells, throughfluorescence microscopy.[13]
NADH can be converted to NAD+ in a reaction catalysed by copper, which requires hydrogen peroxide. Thus, the supply of NAD+ in cells requires mitochondrial copper(II).[14][15]
In rat liver, the total amount of NAD+ and NADH is approximately 1 μmole pergram of wet weight, about 10 times the concentration of NADP+ and NADPH in the same cells.[16] The actual concentration of NAD+ in cellcytosol is harder to measure, with recent estimates in animal cells ranging around 0.3 mM,[17][18] and approximately 1.0 to 2.0 mM inyeast.[19] However, more than 80% of NADH fluorescence in mitochondria is from bound form, so the concentration in solution is much lower.[20]
NAD+ concentrations are highest in the mitochondria, constituting 40% to 70% of the total cellular NAD+.[21] NAD+ in the cytosol is carried into the mitochondrion by a specificmembrane transport protein, since the coenzyme cannotdiffuse across membranes.[22] The intracellularhalf-life of NAD+ was claimed to be between 1–2 hours by one review,[23] whereas another review gave varying estimates based on compartment: intracellular 1–4 hours, cytoplasmic 2 hours, and mitochondrial 4–6 hours.[24]
The balance between the oxidized and reduced forms of nicotinamide adenine dinucleotide is called the NAD+/NADH ratio. This ratio is an important component of what is called theredox state of a cell, a measurement that reflects both the metabolic activities and the health of cells.[25] The effects of the NAD+/NADH ratio are complex, controlling the activity of several key enzymes, includingglyceraldehyde 3-phosphate dehydrogenase andpyruvate dehydrogenase. In healthy mammalian tissues, estimates of the ratio of free NAD+ to NADH in the cytoplasm typically lie around 700:1; the ratio is thus favorable for oxidative reactions.[26][27] The ratio of total NAD+/NADH is much lower, with estimates ranging from 3–10 in mammals.[28] In contrast, theNADP+/NADPH ratio is normally about 0.005, so NADPH is the dominant form of this coenzyme.[29] These different ratios are key to the different metabolic roles of NADH and NADPH.
NAD+ is synthesized through two metabolic pathways. It is produced either in ade novo pathway from amino acids or in salvage pathways by recycling preformed components such asnicotinamide back to NAD+. Although most tissues synthesize NAD+ by the salvage pathway in mammals, much morede novo synthesis occurs in the liver from tryptophan, and in the kidney andmacrophages fromnicotinic acid.[30]
Most organisms synthesize NAD+ from simple components.[4] The specific set of reactions differs among organisms, but a common feature is the generation ofquinolinic acid (QA) from an amino acid – eithertryptophan (Trp) in animals and some bacteria, oraspartic acid (Asp) in some bacteria and plants.[31][32] The quinolinic acid is converted to nicotinic acid mononucleotide (NaMN) by transfer of a phosphoribose moiety. An adenylate moiety is then transferred to form nicotinic acid adenine dinucleotide (NaAD). Finally, the nicotinic acid moiety in NaAD isamidated to a nicotinamide (Nam) moiety, forming nicotinamide adenine dinucleotide.[4]
Despite the presence of thede novo pathway, the salvage reactions are essential in humans; a lack ofvitamin B3 in the diet causes thevitamin deficiency diseasepellagra.[36] This high requirement for NAD+ results from the constant consumption of the coenzyme in reactions such as posttranslational modifications, since the cycling of NAD+ between oxidized and reduced forms in redox reactions does not change the overall levels of the coenzyme.[4]The major source of NAD+ in mammals is the salvage pathway which recycles thenicotinamide produced by enzymes utilizing NAD+.[37] The first step, and the rate-limiting enzyme in the salvage pathway isnicotinamide phosphoribosyltransferase (NAMPT), which producesnicotinamide mononucleotide (NMN).[37] NMN is the immediate precursor to NAD+ in the salvage pathway.[38]
Besides assembling NAD+de novo from simple amino acid precursors, cells also salvage preformed compounds containing a pyridine base. The three vitamin precursors used in these salvage metabolic pathways arenicotinic acid (NA),nicotinamide (Nam) andnicotinamide riboside (NR).[4] These compounds can be taken up from the diet and are termed vitamin B3 orniacin. However, these compounds are also produced within cells and by digestion of cellular NAD+. Some of the enzymes involved in these salvage pathways appear to be concentrated in thecell nucleus, which may compensate for the high level of reactions that consume NAD+ in thisorganelle.[39] There are some reports that mammalian cells can take up extracellular NAD+ from their surroundings,[40] and both nicotinamide and nicotinamide riboside can be absorbed from the gut.[41]
The salvage pathways used inmicroorganisms differ from those ofmammals.[42] Some pathogens, such as the yeastCandida glabrata and the bacteriumHaemophilus influenzae are NAD+auxotrophs – they cannot synthesize NAD+ – but possess salvage pathways and thus are dependent on external sources of NAD+ or its precursors.[43][44] Even more surprising is the intracellularpathogenChlamydia trachomatis, which lacks recognizable candidates for any genes involved in the biosynthesis or salvage of both NAD+ and NADP+, and must acquire these coenzymes from itshost.[45]
Nicotinamide adenine dinucleotide has several essential roles inmetabolism. It acts as acoenzyme inredox reactions, as a donor of ADP-ribose moieties inADP-ribosylation reactions, as a precursor of thesecond messenger moleculecyclic ADP-ribose, as well as acting as a substrate for bacterialDNA ligases and a group of enzymes calledsirtuins that use NAD+ to removeacetyl groups from proteins. In addition to these metabolic functions, NAD+ emerges as an adenine nucleotide that can be released from cells spontaneously and by regulated mechanisms,[47][48] and can therefore have importantextracellular roles.[48]
The main role of NAD+ in metabolism is the transfer of electrons from one molecule to another. Reactions of this type are catalyzed by a large group of enzymes calledoxidoreductases. The correct names for these enzymes contain the names of both their substrates: for exampleNADH-ubiquinone oxidoreductase catalyzes the oxidation of NADH bycoenzyme Q.[49] However, these enzymes are also referred to asdehydrogenases orreductases, with NADH-ubiquinone oxidoreductase commonly being calledNADH dehydrogenase or sometimescoenzyme Q reductase.[50]
There are many different superfamilies of enzymes that bind NAD+ / NADH. One of the most common superfamilies includes astructural motif known as theRossmann fold.[51][52] The motif is named afterMichael Rossmann, who was the first scientist to notice how common this structure is within nucleotide-binding proteins.[53]
In this diagram, the hydride acceptor C4 carbon is shown at the top. When the nicotinamide ring lies in the plane of the page with the carboxy-amide to the right, as shown, the hydride donor lies either "above" or "below" the plane of the page. If "above" hydride transfer is class A, if "below" hydride transfer is class B.[55]
When bound in the active site of an oxidoreductase, the nicotinamide ring of the coenzyme is positioned so that it can accept a hydride from the other substrate. Depending on the enzyme, the hydride donor is positioned either "above" or "below" the plane of the planar C4 carbon, as defined in the figure. Class A oxidoreductases transfer the atom from above; class B enzymes transfer it from below. Since the C4 carbon that accepts the hydrogen isprochiral, this can be exploited inenzyme kinetics to give information about the enzyme's mechanism. This is done by mixing an enzyme with a substrate that hasdeuterium atoms substituted for the hydrogens, so the enzyme will reduce NAD+ by transferring deuterium rather than hydrogen. In this case, an enzyme can produce one of twostereoisomers of NADH.[55]
Despite the similarity in how proteins bind the two coenzymes, enzymes almost always show a high level of specificity for either NAD+ or NADP+.[56] This specificity reflects the distinct metabolic roles of the respective coenzymes, and is the result of distinct sets ofamino acid residues in the two types of coenzyme-binding pocket. For instance, in the active site of NADP-dependent enzymes, anionic bond is formed between a basic amino acid side-chain and the acidic phosphate group of NADP+. On the converse, in NAD-dependent enzymes the charge in this pocket is reversed, preventing NADP+ from binding. However, there are a few exceptions to this general rule, and enzymes such asaldose reductase,glucose-6-phosphate dehydrogenase, andmethylenetetrahydrofolate reductase can use both coenzymes in some species.[57]
The redox reactions catalyzed by oxidoreductases are vital in all parts of metabolism, but one particularly important area where these reactions occur is in the release of energy from nutrients. Here, reduced compounds such asglucose andfatty acids are oxidized, thereby releasing energy. This energy is transferred to NAD+ by reduction to NADH, as part ofbeta oxidation,glycolysis, and thecitric acid cycle. Ineukaryotes the electrons carried by the NADH that is produced in thecytoplasm are transferred into themitochondrion (to reduce mitochondrial NAD+) bymitochondrial shuttles, such as themalate-aspartate shuttle.[58] The mitochondrial NADH is then oxidized in turn by theelectron transport chain, which pumps protons across a membrane and generates ATP throughoxidative phosphorylation.[59] These shuttle systems also have the same transport function inchloroplasts.[60]
Since both the oxidized and reduced forms of nicotinamide adenine dinucleotide are used in these linked sets of reactions, the cell maintains significant concentrations of both NAD+ and NADH, with the high NAD+/NADH ratio allowing this coenzyme to act as both an oxidizing and a reducing agent.[61] In contrast, the main function of NADPH is as a reducing agent inanabolism, with this coenzyme being involved in pathways such asfatty acid synthesis andphotosynthesis. Since NADPH is needed to drive redox reactions as a strong reducing agent, the NADP+/NADPH ratio is kept very low.[61]
Although it is important in catabolism, NADH is also used in anabolic reactions, such asgluconeogenesis.[62] This need for NADH in anabolism poses a problem for prokaryotes growing on nutrients that release only a small amount of energy. For example,nitrifying bacteria such asNitrobacter oxidize nitrite to nitrate, which releases sufficient energy to pump protons and generate ATP, but not enough to produce NADH directly.[63] As NADH is still needed for anabolic reactions, these bacteria use anitrite oxidoreductase to produce enoughproton-motive force to run part of the electron transport chain in reverse, generating NADH.[64]
The coenzyme NAD+ is also consumed in ADP-ribose transfer reactions. For example, enzymes calledADP-ribosyltransferases add the ADP-ribose moiety of this molecule to proteins, in aposttranslational modification calledADP-ribosylation.[65] ADP-ribosylation involves either the addition of a single ADP-ribose moiety, inmono-ADP-ribosylation, or the transferral of ADP-ribose to proteins in long branched chains, which is calledpoly(ADP-ribosyl)ation.[66] Mono-ADP-ribosylation was first identified as the mechanism of a group of bacterialtoxins, notablycholera toxin, but it is also involved in normalcell signaling.[67][68] Poly(ADP-ribosyl)ation is carried out by thepoly(ADP-ribose) polymerases.[66][69] The poly(ADP-ribose) structure is involved in the regulation of several cellular events and is most important in thecell nucleus, in processes such asDNA repair andtelomere maintenance.[69] In addition to these functions within the cell, a group ofextracellular ADP-ribosyltransferases has recently been discovered, but their functions remain obscure.[70]NAD+ may also be added onto cellularRNA as a 5'-terminal modification.[71]
NAD+ is also consumed by different NAD+-consuming enzymes, such asCD38,CD157,PARPs and the NAD-dependentdeacetylases (sirtuins, such asSir2.[75]).[76] These enzymes act by transferring anacetyl group from their substrate protein to the ADP-ribose moiety of NAD+; this cleaves the coenzyme and releases nicotinamide and O-acetyl-ADP-ribose. The sirtuins mainly seem to be involved in regulatingtranscription through deacetylating histones and alteringnucleosome structure.[77] However, non-histone proteins can be deacetylated by sirtuins as well. These activities of sirtuins are particularly interesting because of their importance in the regulation ofaging.[78][79]
Other NAD-dependent enzymes include bacterialDNA ligases, which join two DNA ends by using NAD+ as a substrate to donate anadenosine monophosphate (AMP) moiety to the 5' phosphate of one DNA end. This intermediate is then attacked by the 3' hydroxyl group of the other DNA end, forming a newphosphodiester bond.[80] This contrasts witheukaryotic DNA ligases, which use ATP to form the DNA-AMP intermediate.[81]
Li et al. have found that NAD+ directly regulates protein-protein interactions.[82] They also show that one of the causes of age-related decline in DNA repair may be increased binding of the proteinDBC1 (Deleted in Breast Cancer 1) toPARP1 (poly[ADP–ribose] polymerase 1) as NAD+ levels decline during aging.[82] The decline in cellular concentrations of NAD+ during aging likely contributes to theaging process and to thepathogenesis of the chronic diseases of aging.[83] Thus, the modulation of NAD+ may protect against cancer, radiation, and aging.[82]
In recent years, NAD+ has also been recognized as anextracellular signaling molecule involved in cell-to-cell communication.[48][84][85] NAD+ is released fromneurons inblood vessels,[47]urinary bladder,[47][86]large intestine,[87][88] from neurosecretory cells,[89] and from brainsynaptosomes,[90] and is proposed to be a novelneurotransmitter that transmits information fromnerves to effector cells insmooth muscle organs.[87][88] In plants, the extracellular nicotinamide adenine dinucleotide induces resistance to pathogen infection and the first extracellular NAD receptor has been identified.[91] Further studies are needed to determine the underlying mechanisms of its extracellular actions and their importance for human health and life processes in other organisms.
The enzymes that make and use NAD+ and NADH are important in bothpharmacology and the research into future treatments for disease.[92]Drug design and drug development exploits NAD+ in three ways: as a direct target of drugs, by designingenzyme inhibitors or activators based on its structure that change the activity of NAD-dependent enzymes, and by trying to inhibit NAD+ biosynthesis.[93]
Because cancer cells utilize increasedglycolysis, and because NAD enhances glycolysis, nicotinamide phosphoribosyltransferase (NAD salvage pathway) is often amplified in cancer cells.[94][95]
Since many oxidoreductases use NAD+ and NADH as substrates, and bind them using a highly conserved structural motif, the idea that inhibitors based on NAD+ could be specific to one enzyme is surprising.[101] However, this can be possible: for example, inhibitors based on the compoundsmycophenolic acid andtiazofurin inhibitIMP dehydrogenase at the NAD+ binding site. Because of the importance of this enzyme inpurine metabolism, these compounds may be useful as anti-cancer, anti-viral, orimmunosuppressive drugs.[101][102] Other drugs are not enzyme inhibitors, but instead activate enzymes involved in NAD+ metabolism.Sirtuins are a particularly interesting target for such drugs, since activation of these NAD-dependent deacetylases extends lifespan in some animal models.[103] Compounds such asresveratrol increase the activity of these enzymes, which may be important in their ability to delay aging in both vertebrate,[104] and invertebratemodel organisms.[105][106] In one experiment, mice given NAD for one week had improved nuclear-mitochrondrial communication.[107]
Because of the differences in themetabolic pathways of NAD+ biosynthesis between organisms, such as between bacteria and humans, this area of metabolism is a promising area for the development of newantibiotics.[108][109] For example, the enzymenicotinamidase, which converts nicotinamide to nicotinic acid, is a target for drug design because this enzyme is absent in humans but present in yeast and bacteria.[42]
In bacteriology, NAD, sometimes referred to factor V, is used as a supplement to culture media for somefastidious bacteria.[110]
High-cost unlicensed infusions of NAD+ have been claimed in the UK to be "clinically proven" and "effective" treatment foralcoholism anddrug abuse. NAD+ is not approved orlicensed for medical use in the UK; there are likely breachesof advertising and medicines rules, and no proof that treatments work. Medical experts say "It's complete nonsense" ... "It's untested and unproven. We don't know anything about its efficacy or long-term safety". A November 2024 study, cited 700 times, claiming that NAD+ levels inlab rats decreased with age was withdrawn after images were found to have been manipulated, and underlying data was not provided to the publishers on request.[111]
The coenzyme NAD+ was first discovered by the British biochemistsArthur Harden andWilliam John Young in 1906.[112] They noticed that adding boiled and filteredyeast extract greatly acceleratedalcoholic fermentation in unboiled yeast extracts. They called the unidentified factor responsible for this effect acoferment. Through a long and difficult purification from yeast extracts, this heat-stable factor was identified as anucleotide sugar phosphate byHans von Euler-Chelpin.[113] In 1936, the German scientistOtto Heinrich Warburg showed the function of the nucleotide coenzyme in hydride transfer and identified the nicotinamide portion as the site of redox reactions.[114]
Vitamin precursors of NAD+ were first identified in 1938, whenConrad Elvehjem showed that liver has an "anti-black tongue" activity in the form of nicotinamide.[115] Then, in 1939, he provided the first strong evidence thatnicotinic acid is used to synthesize NAD+.[116] In the early 1940s,Arthur Kornberg was the first to detect an enzyme in the biosynthetic pathway.[117] In 1949, the American biochemists Morris Friedkin andAlbert L. Lehninger proved that NADH linked metabolic pathways such as thecitric acid cycle with the synthesis of ATP in oxidative phosphorylation.[118] In 1958, Jack Preiss and Philip Handler discovered the intermediates and enzymes involved in the biosynthesis of NAD+;[119][120] salvage synthesis from nicotinic acid is termed the Preiss-Handler pathway. In 2004,Charles Brenner and co-workers uncovered thenicotinamide riboside kinase pathway to NAD+.[121]
The non-redox roles of NAD(P) were discovered later.[3] The first to be identified was the use of NAD+ as the ADP-ribose donor in ADP-ribosylation reactions, observed in the early 1960s.[122] Studies in the 1980s and 1990s revealed the activities of NAD+ and NADP+ metabolites in cell signaling – such as the action ofcyclic ADP-ribose, which was discovered in 1987.[123]
The metabolism of NAD+ remained an area of intense research into the 21st century, with interest heightened after the discovery of the NAD+-dependent protein deacetylases calledsirtuins in 2000, by Shin-ichiro Imai and coworkers in the laboratory ofLeonard P. Guarente.[124] In 2009 Imai proposed the "NAD World" hypothesis that key regulators of aging and longevity in mammals aresirtuin 1 and the primary NAD+ synthesizing enzymenicotinamide phosphoribosyltransferase (NAMPT).[125] In 2016 Imai expanded his hypothesis to "NAD World 2.0", which postulates that extracellular NAMPT fromadipose tissue maintains NAD+ in thehypothalamus (the control center) in conjunction withmyokines fromskeletal muscle cells.[126] In 2018, Napa Therapeutics was formed to develop drugs against a novel aging-related target based on the research in NAD metabolism conducted in the lab ofEric Verdin.[127]
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