The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conservedtranscription factor that binds with high specificity toCCAAT motifs in thepromoter regions in a variety of genes. This gene product, subunit C, forms a tight dimer with the B subunit (NFYB), a prerequisite for subunit A (NFYA) association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain ahistone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. Additional regulation, preliminarily supported by the EST database, may be represented by alternative splicing in this subunit.[7]
TwomicroRNAs; miR-30c and miR-30e are located withinintrons of thenfyc gene. These microRNAs are actively transcribed in human insulin-producingbeta cells in thepancreatic islets that also show high expression of nfyc andCDH1 genes. The expression of these intronic microRNAs is essential for maintaining the differentiated phenotype of human islet beta cells. Inhibition of miR-30 family microRNAs inducesepithelial-mesenchymal transition of human pancreatic islet cells.[8]
^"Human PubMed Reference:".National Center for Biotechnology Information, U.S. National Library of Medicine.
^"Mouse PubMed Reference:".National Center for Biotechnology Information, U.S. National Library of Medicine.
^Sinha S, Maity SN, Seldin MF, de Crombrugghe B (Feb 1997). "Chromosomal assignment and tissue expression of CBF-C/NFY-C, the third subunit of the mammalian CCAAT-binding factor".Genomics.37 (2):260–3.doi:10.1006/geno.1996.0555.PMID8921405.
^Bellorini M, Zemzoumi K, Farina A, Berthelsen J, Piaggio G, Mantovani R (Aug 1997). "Cloning and expression of human NF-YC".Gene.193 (1):119–25.doi:10.1016/S0378-1119(97)00109-1.PMID9249075.
Dmitrenko V, Garifulin O, Kavsan V (1997). "Isolation and sequence analysis of the cDNA encoding subunit C of human CCAAT-binding transcription factor".Gene.197 (1–2):161–3.doi:10.1016/S0378-1119(97)00255-2.PMID9332362.
Izumi H, Molander C, Penn LZ, Ishisaki A, Kohno K, Funa K (2001). "Mechanism for the transcriptional repression by c-Myc on PDGF beta-receptor".J. Cell Sci.114 (Pt 8):1533–44.doi:10.1242/jcs.114.8.1533.PMID11282029.
Hirose T, Sowa Y, Takahashi S, Saito S, Yasuda C, Shindo N, Furuichi K, Sakai T (2003). "p53-independent induction of Gadd45 by histone deacetylase inhibitor: coordinate regulation by transcription factors Oct-1 and NF-Y".Oncogene.22 (49):7762–73.doi:10.1038/sj.onc.1207091.PMID14586402.S2CID1826456.
