Intron-encoded endonuclease I-Sce I is ahoming endonuclease. The enzyme is used in biotechnology as ameganuclease. It recognises an 18-base pair sequence TAGGGATAACAGGGTAAT and leaves a 4 base pair 3' hydroxyl overhang. It is a rare cuttingendonuclease. Statistically an 18-bp sequence will occur once in every 6.9*10¹⁰ base pairs (a frequency of 1 in 4¹⁸). This sequence does not normally occur in a human or mouse genome.^[1]

I-SceI is coded byintrons. It is present in themitochondria ofyeastSaccharomyces cerevisiae.

Thismolecular orcell biology article is astub. You can help Wikipedia byadding missing information.

• v
• t
• e

• Restriction enzymes
• Molecular and cellular biology stubs

• Webarchive template wayback links
• Protein pages needing a picture
• All stub articles