Lysogeny, or thelysogenic cycle, is one of two cycles ofviral reproduction (thelytic cycle being the other). Lysogeny is characterized by integration of thebacteriophage nucleic acid into the hostbacterium's genome or formation of a circularreplicon in the bacterialcytoplasm. In this condition the bacterium continues to live and reproduce normally, while the bacteriophage lies in a dormant state in the host cell. The genetic material of the bacteriophage, called aprophage, can be transmitted to daughter cells at each subsequent cell division, and later events (such asUV radiation or the presence of certain chemicals) can release it, causing proliferation of new phages via the lytic cycle.[1]
Lysogenic cycles can also occur ineukaryotes, although the method of DNA incorporation is not fully understood. For instance, theHIV viruses can either infect humans lytically, orlay dormant (lysogenic) as part of the infected cells' genome, keeping the ability to return to lysis at a later time.[2]
The difference between lysogenic and lytic cycles is that, in lysogenic cycles, the spread of the viral DNA occurs through the usual prokaryotic reproduction, whereas a lytic cycle is more immediate in that it results in many copies of the virus being created very quickly and the cell is destroyed. One key difference between the lytic cycle and the lysogenic cycle is that the latter does not lyse the host cell straight away.[3] Phages that replicate only via the lytic cycle are known as virulent phages while phages that replicate using both lytic and lysogenic cycles are known astemperate phages.[1]
In the lysogenic cycle, the phage DNA first integrates into the bacterial chromosome to produce the prophage. When the bacterium reproduces, the prophage is also copied and is present in each of the daughter cells. The daughter cells can continue to replicate with the prophage present or the prophage can exit the bacterial chromosome to initiate the lytic cycle.[1] In the lysogenic cycle the host DNA is not hydrolyzed but in the lytic cycle the host DNA is hydrolyzed in the lytic phase.
Bacteriophages are viruses that infect and replicate within a bacterium.Temperate phages (such aslambda phage) can reproduce using both thelytic and the lysogenic cycle.[4]
How a phage decides which cycle to enter depends on a variety of factors.[5] For instance, if there are several other infecting phages (or if there is a high multiplicity), it is likely that the phage will use the lysogenic cycle. This may be useful in helping reduce the overall phage-to-host ratio and therefore preventing the phages from killing their hosts, also thereby increasing the phage's potential for survival, making this a form of natural selection. A phage may exit the chromosome and enter the lytic cycle if it is exposed to DNA-damaging agents, such as UV radiation and chemicals. Other factors with the potential to induce temperate phage release include temperature, pH, osmotic pressure, and low nutrient concentration.[6] However, phages may also re-enter the lytic cycle spontaneously.[7]
It is sometimes possible to detect which cycle a phage enters by looking at the plaque morphology in bacterial plate culture.[8] Generally, clearer plaques indicate more efficient lysis, while cloudy or turbid plaques indicate less efficient lysis. Turbid plaques may indicate that a phage can go through the lysogenic cycle, however there are other reasons that plaques may appear turbid.
Detection methods of phages released from the lysogenic cycle include electron microscopy, DNA extraction, or propagation on sensitive strains.[6]
Via the lysogenic cycle, the bacteriophage's genome is not expressed and is instead integrated into the bacteria's genome to form theprophage.[9] In its inactive form, a prophage gets passed on each time the host cell divides. If prophages become active, they can exit the bacterial chromosome and enter the lytic cycle, where they undergo DNA copying, protein synthesis, phage assembly, and lysis.[5]Since the bacteriophage's genetic information is incorporated into the bacteria's genetic information as a prophage, the bacteriophage replicates passively as the bacterium divides to form daughter bacteria cells.[9] In this scenario, the daughter bacteria cells contain prophage and are known aslysogens. Lysogens can remain in the lysogenic cycle for many generations but can switch to the lytic cycle at any time via a process known as induction.[9] During induction, prophage DNA is excised from the bacterial genome and is transcribed and translated to make coat proteins for the virus and regulate lytic growth.[9]
An example of a virus that uses the lysogenic cycle to its advantage is the Herpes Simplex Virus.[11] After first entering the lytic cycle and infecting a human host, it enters the lysogenic cycle. This allows it to travel to the nervous system's sensory neurons and remain undetected for long periods of time. In the case of genital herpes, latency is established in lumbosacral dorsal root ganglia, spinal nerve neurons.[12] The herpes virus can then exit this dormant stage and re-enter the lytic cycle, causing disease symptoms. Thus, while herpes viruses can enter both the lytic and lysogenic cycles, latency allows the virus to survive and evade detection by the immune system due to low viral gene expression.
The model organism for studying lysogeny is the lambda phage. Prophage integration (also known as homologous recombination), maintenance of lysogeny, induction, and control of phage genome excision in induction is described in detail in thelambda phage article.[13]
Bacteriophages are parasitic because they infect their hosts, use bacterial machinery to replicate, and ultimately lyse the bacteria. Temperate phages can lead to both advantages and disadvantages for their hosts via the lysogenic cycle. During the lysogenic cycle, the virus genome is incorporated as prophage and a repressor prevents viral replication. Nonetheless, a temperate phage can escape repression to replicate, produce viral particles, and lyse the bacteria.[14] The temperate phage escaping repression would be a disadvantage for the bacteria. On the other hand, the prophage may transfergenes that enhance host virulence and resistance to the immune system. Also, the repressor produced by the prophage that prevents prophage genes from being expressed confers immunity for the host bacteria from lytic infection by related viruses.[14]
Another system,arbitrium, has recently been described for bacteriophages infecting severalBacillus species, in which the decision between lysis and lysogeny is transmitted between bacteria by apeptide factor.[15][16]
In some interactions between lysogenic phages and bacteria, the lysogenic conversion may occur, which can also be called phage conversion. It is when a temperatephage induces a change in thephenotype of the infectedbacteria that is not part of a usual phage cycle. Changes can often involve the external membrane of the cell by making it impervious to other phages or even by increasing the pathogenic capability of the bacteria for a host. In this way, temperate bacteriophages also play a role in the spread ofvirulence factors, such as exotoxins and exoenzymes, amongst bacteria. This change then stays in the genome of the infected bacteria and is copied and passed down to daughter cells.
Lysogenic conversion has shown to enablebiofilm formation inBacillus anthracis.[17] Strains ofB. anthracis cured of all phage were unable to form biofilms, which are surface-adhered bacterial communities that enable bacteria to better access nutrients and survive environmental stresses.[18] In addition to biofilm formation inB. anthracis, lysogenic conversion ofBacillus subtilis,Bacillus thuringiensis, andBacillus cereus has shown an enhanced rate or extent of sporulation.[17] Sporulation producesendospores, which are metabolically dormant forms of the bacteria that are highly resistant to temperature, ionizing radiation, desiccation, antibiotics, and disinfectants.[17]
Non-virulent bacteria have also been shown to transform into highly virulent pathogens through lysogenic conversion with the virulence factors carried on the lysogenic prophage.[19] Virulence genes carried within prophages as discrete autonomous genetic elements, known asmorons, confer an advantage to the bacteria that indirectly benefits the virus through enhanced lysogen survival.[17]
Examples:
Strategies to combat certain bacterial infections by blocking prophage induction (the transition from thelytic cycle to the lysogenic cycle) by eliminatingin vivo induction agents have been proposed.[19]Reactive oxygen species (ROS), such as hydrogen peroxide, are strong oxidizing agents that can decompose into free radicals and cause DNA damage to bacteria, which lead to prophage induction.[19] One potential strategy to combat prophage induction is through the use ofglutathione, a strongantioxidant that can remove free radical intermediates.[19] Another approach could be to cause overexpression of CI repressor since prophage induction only occurs when the concentration of CI repressor is too low.[19]
