High Five cells have become one of the most commonly used cell lines for recombinantprotein expression usingbaculovirus ortransfection, and have been demonstrated to express more recombinant protein than other lepidopteran cell lines, such asSf9 cells.[2][3][4][5][6][7][8] The High Five cells have been used to produce the VLP-based HPV vaccineCervarix.[9]
They can be grown in the absence of serum, and can be cultured in a loose attached state or in suspension[10] High Five cells produce abundantmicroRNAs (miRNAs),small interfering RNAs (siRNAs), andPIWI-interacting RNAs (piRNAs), making it suitable to study all three types of small silencing RNAs.[11]
The High Five cell line, like other cell lines,[12] has been found to host a non-pathogenic adventitious alphanodavirus. Researchers at the Boyce Thompson Institute were able to isolate virus-free sub-clones, namedTnao38[13][14] andTnms42.[15]
^Wickham TJ, Davis T, Granados RR, Shuler ML, Wood HA (1992). "Screening of insect cell lines for the production of recombinant proteins and infectious virus in the baculovirus expression system".Biotechnol. Prog.8 (5):391–396.doi:10.1021/bp00017a003.PMID1369220.S2CID41111655.
^Davis TR, Trotter KM, Granados RR, Wood HA (1992). "Baculovirus expression of alkaline phosphatase as a reporter gene for evaluation of production, glycosylation and secretion".Bio/Technology.10 (10):1148–1150.doi:10.1038/nbt1092-1148.PMID1368794.S2CID5978915.
^Wickham TJ, Nemerow GR (1993). "Optimization of growth methods and recombinant protein production in BTI-Tn-5B1-4 insect cells using the baculovirus expression system".Biotechnol. Prog.9 (1):25–30.doi:10.1021/bp00019a004.PMID7764044.S2CID21157206.
