The namegranule cell has been used for a number of different types ofneurons whose only common feature is that they all have very smallcell bodies. Granule cells are found within thegranular layer of thecerebellum, thedentate gyrus of thehippocampus, the superficial layer of thedorsal cochlear nucleus, theolfactory bulb, and thecerebral cortex.
Cerebellar granule cells account for the majority ofneurons in the human brain.[1] These granule cells receive excitatory input frommossy fibers originating frompontinenuclei. Cerebellar granule cells project up through thePurkinje layer into themolecular layer where they branch out into parallel fibers that spread throughPurkinje celldendritic arbors. These parallel fibers form thousands of excitatorygranule-cell–Purkinje-cell synapses onto the intermediate and distal dendrites of Purkinje cells usingglutamate as aneurotransmitter.
Layer 4 granule cells of the cerebral cortex receive inputs from thethalamus and send projections to supragranular layers 2–3, but also to infragranular layers of the cerebral cortex.
Granule cells in different brain regions are both functionally and anatomically diverse: the only thing they have in common is smallness. For instance, olfactory bulb granule cells areGABAergic and axonless, while granule cells in the dentate gyrus haveglutamatergic projectionaxons. These two populations of granule cells are also the only major neuronal populations that undergoadult neurogenesis, while cerebellar and cortical granule cells do not.Granule cells (save for those of the olfactory bulb) have a structure typical of a neuron consisting ofdendrites, asoma (cell body) and an axon.
Dendrites: Each granule cell has 3 – 4 stubby dendrites which end in a claw. Each of the dendrites are only about 15μm in length.
Soma: Granule cells all have a small soma diameter of approximately 10 μm.
Axon: Each granule cell sends a single axon onto the Purkinje cell dendritic tree.[citation needed] The axon has an extremely narrow diameter: ½ micrometre.
Synapse: 100–300,000 granule cell axonssynapse onto a singlePurkinje cell.[citation needed]
The existence ofgap junctions between granule cells allows multipleneurons to be coupled to one another, allowing multiple cells to act in synchrony, and allows signalling functions necessary for granule cell development to occur.[2]
The granule cells, produced by therhombic lip, are found in the granule cell layer of thecerebellar cortex. They are small and numerous. They are characterized by a very small soma and several short dendrites which terminate with claw-shaped endings. In thetransmission electron microscope, these cells are characterized by a darkly stained nucleus surrounded by a thin rim of cytoplasm. The axon ascends into the molecular layer where it splits to formparallel fibers.[1]
The principal cell type of thedentate gyrus is the granule cell. The dentate gyrus granule cell has an elliptical cell body with a width of approximately 10 μm and a height of 18μm.[3]
The granule cell has a characteristic cone-shaped tree of spiny apicaldendrites. The dendrite branches project throughout the entire molecular layer, and the furthest tips of the dendritic tree end at thehippocampal fissure or at the ventricular surface.[4] The granule cells are tightly packed in the granular cell layer of the dentate gyrus.
The granule cells in thedorsal cochlear nucleus are small neurons with two or three short dendrites that give rise to a few branches with expansions at the terminals. The dendrites are short with claw-like endings that formglomeruli to receivemossy fibers, similar to cerebellar granule cells.[5] Its axon projects to the molecular layer of the dorsal cochlear nucleus where it forms parallel fibers, also similar to cerebellar granule cells.[6]The dorsal cochlear granule cells are small excitatoryinterneurons which are developmentally related and thus resemble the cerebellar granule cell.
The main intrinsic granule cell in the vertebrateolfactory bulb lacks an axon (as does the accessory neuron). Each cell gives rise to short central dendrites and a single long apical dendrite that expands into the granule cell layer and enters themitral cell body layer. The dendrite branches terminate within the outer plexiform layer among the dendrites in theolfactory tract.[7]In the mammalian olfactory bulb, granule cells can process both synaptic input and output due to the presence of large spines.[8]
Cerebellar granule cells receive excitatory input from 3 or 4mossy fibers originating frompontine nuclei. Mossy fibers make an excitatory connection onto granule cells, which causes the granule cells to fire an action potential.
The axon of a cerebellar granule cell splits to form aparallel fiber which innervatesPurkinje cells. The vast majority of granule cell axonal synapses are found on the parallel fibers.[9]
The parallel fibers are sent up through thePurkinje layer into themolecular layer where they branch out and spread through Purkinje cell dendritic arbors. These parallel fibers form thousands of excitatoryGranule-cell-Purkinje-cell synapses onto the dendrites of Purkinje cells.
This connection is excitatory asglutamate is released.
The parallel fibers and ascending axon synapses from the same granule cell fire in synchrony which results in excitatory signals. In the cerebellar cortex there are a variety of inhibitory neurons (interneurons). The only excitatory neurons present in the cerebellar cortex are granule cells.[10]
Plasticity of the synapse between a parallel fiber and a Purkinje cell is believed to be important formotor learning.[11] The function of cerebellar circuits is entirely dependent on processes carried out by the granular layer. Therefore, the function of granule cells determines the cerebellar function as a whole.[12]
Granule cell dendrites also synapse with distinctive unmyelinated axons whichSantiago Ramón y Cajal calledmossy fibers[4]Mossy fibers andgolgi cells both make synaptic connections with granule cells. Together these cells form the glomeruli.[10]
Granule cells are subject tofeed-forward inhibition: granule cells excite Purkinje cells but also excite GABAergic interneurons that inhibit Purkinje cells.
Granule cells are also subject tofeedback inhibition: Golgi cells receive excitatory stimuli from granule cells and in turn send back inhibitory signals to the granule cell.[13]
Mossy fiber input codes are conserved duringsynaptic transmission between granule cells, suggesting thatinnervation is specific to the input that is received.[14] Granule cells do not just relay signals from mossy fibers, rather they perform various, intricate transformations which are required in the spatiotemporal domain.[10]
Each granule cell is receiving an input from two different mossy fiber inputs. The input is thus coming from two different places as opposed to the granule cell receiving multiple inputs from the same source.
The differences in mossy fibers that are sending signals to the granule cells directly effects the type of information that granule cells translate to Purkinje cells. The reliability of this translation will depend on the reliability ofsynaptic activity in granule cells and on the nature of the stimulus being received.[15] The signal a granule cell receives from aMossy fiber depends on the function of the mossy fiber itself. Therefore, granule cells are able to integrate information from the different mossy fibers and generate new patterns of activity.[15]
Different patterns of mossy fiber input will produce unique patterns of activity in granule cells that can be modified by a teaching signal conveyed by theclimbing fiber input.David Marr andJames Albus suggested that the cerebellum operates as an adaptive filter, altering motor behaviour based on the nature of the sensory input.
Since multiple (~200,000) granule cells synapse onto a single Purkinje cell, the effects of each parallel fiber can be altered in response to a “teacher signal” from the climbing fiber input.
David Marr suggested that the granule cells encodecombinations of mossy fiber inputs. In order for the granule cell to respond, it needs to receive active inputs from multiple mossy fibers. The combination of multiple inputs results in the cerebellum being able to make more precise distinctions between input patterns than a single mossy fiber would allow.[16] The cerebellar granule cells also play a role in orchestrating the tonic conductances which control sleep in conjunction with the ambient levels ofGABA which are found in the brain.
Loss of dentate gyrus neurons from thehippocampus results in spatial memory deficits. Therefore, dentate granule cells are thought to function in the formation of spatial memories[17] and of episodic memories.[18]Immature and mature dentate granule cells have distinct roles in memory function. Adult-born granule cells are thought to be involved in pattern separation whereas old granule cells contribute to rapid pattern completion.[19]
Pyramidal cells from theprimary auditory cortex project directly on to the cochlear nucleus. This is important in the acousticstartle reflex, in which the pyramidal cells modulate the secondary orientation reflex and the granule cell input is responsible for appropriate orientation.[20] This is because the signals received by the granule cells contain information about the head position. Granule cells in the dorsal cochlear nucleus play a role in the perception and response to sounds in our environment.
Inhibition generated by granule cells, the most common GABAergic cell type in the olfactory bulb, plays a critical role in shaping the output of the olfactory bulb.[21] There are two types of excitatory inputs received byGABAergic granule cells; those activated by anAMPA receptor and those activated by aNMDA receptor. This allows the granule cells to regulate the processing of the sensory input in the olfactory bulb.[21] Theolfactory bulb transmits smell information from the nose to the brain, and is thus necessary for a proper sense of smell. Granule cells in the olfactory bulb have also been found to be important in forming memories linked with scents.[22]
Calcium dynamics are essential for several functions of granule cells such as changingmembrane potential,synaptic plasticity,apoptosis, and regulation ofgene transcription.[10] The nature of the calcium signals that control the presynaptic and postsynaptic function of the olfactory bulb granule cells spines is mostly unknown.[8]
Granule neurons have high levels of the neuronal isoform ofnitric oxide synthase. This enzyme is dependent on the presence of calcium and is responsible for the production ofnitric oxide (NO). Thisneurotransmitter is a negative regulator of granule cell precursor proliferation which promotes the differentiation of different granule cells. NO regulates interactions between granule cells andglia[10] and is essential for protecting the granule cells from damage. NO is also responsible forneuroplasticity andmotor learning.[23]
TrkB is responsible for the maintenance of normal synaptic connectivity of the dentate granule cells. TrkB also regulates the specificmorphology (biology) of the granule cells and is thus said to be important in regulating neuronal development, neuronal plasticity, learning, and the development ofepilepsy.[24] The TrkB regulation of granule cells is important in preventing memory deficits and limbic epilepsy. This is due to the fact that dentate granule cells play a critical role in the function of the entorhinal-hippocampal circuitry in health and disease. Dentate granule cells are situated to regulate the flow of information into thehippocampus, a structure required for normal learning and memory.[24]
Bothepilepsy anddepression show a disrupted production of adult-born hippocampal granule cells.[25] Epilepsy is associated with increased production - but aberrant integration - of new cells early in the disease and decreased production late in the disease.[25] Aberrant integration of adult-generated cells during the development of epilepsy may impair the ability of the dentate gyrus to prevent excess excitatory activity from reaching hippocampalpyramidal cells, thereby promoting seizures.[25] Long-lasting epileptic seizure stimulate dentate granule cell neurogenesis. These newly born dentate granule cells may result in aberrant connections that result in the hippocampal network plasticity associated withepileptogenesis.[26]
Patients withAlzheimer's have shorter granule cell dendrites. Furthermore, the dendrites were less branched and had fewer spines than those in patients not with Alzheimer's.[27] However, granule cell dendrites are not an essential component ofsenile plaques and these plaques have no direct effect on granule cells in the dentate gyrus. The specific neurofibrillary changes of dentate granule cells occur in patients with Alzheimer's,Lewy body variant andprogressive supranuclear palsy.[28]
List of distinct cell types in the adult human body