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Detyrosination

From Wikipedia, the free encyclopedia

Detyrosination is a form ofposttranslational modification that occurs on alpha-tubulin.[1] It consists of the removal of theC-terminaltyrosine to expose aglutamate at the newly formed C-terminus. Tubulin polymers, calledmicrotubules, that contain detyrosinated alpha-tubulin are usually referred to asGlu-microtubules while unmodified polymers are calledTyr-microtubules.

The detyrosynating activity was first identified in the late 1970s.[2] It is a slow acting enzyme that uses polymeric tubulin as a substrate. As a result, only stabilized microtubules accumulate this particular modification. Tubulin detyrosination is reversed by thetubulin-tyrosine ligase,[3] which acts only on alpha-tubulin monomer. Since the majority of microtubules are very dynamic, they do not contain much detyrosinated tubulin.

See also

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References

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Vasohibins/SVBP are tubulin carboxypeptidases (TCPs) that regulate neuron differentiation.

Aillaud C, Bosc C, Peris L, Bosson A, Heemeryck P, Van Dijk J, Le Friec J, Boulan B, Vossier F, Sanman LE, Syed S, Amara N, Couté Y, Lafanechère L, Denarier E, Delphin C, Pelletier L, Humbert S, Bogyo M, Andrieux A, Rogowski K, Moutin MJ.

Science. 2017 Dec 15;358(6369):1448-1453. doi: 10.1126/science.aao4165. Epub 2017 Nov 16.

PMID: 29146868

  1. ^Janke C, Bulinski JC (2011). "Post-translational regulation of the microtubule cytoskeleton: mechanisms and functions".Nat Rev Mol Cell Biol.12 (12):773–86.doi:10.1038/nrm3227.PMID 22086369.
  2. ^Hallak ME, Rodriguez JA, Barra HS, Caputto R (1977)."Release of tyrosine from tyrosinated tubulin. Some common factors that affect this process and the assembly of tubulin".FEBS Lett.73 (2):147–50.Bibcode:1977FEBSL..73..147H.doi:10.1016/0014-5793(77)80968-x.PMID 838053.
  3. ^Ersfeld K, Wehland J, Plessmann U, Dodemont H, Gerke V, Weber K (1993)."Characterization of the tubulin-tyrosine ligase".J Cell Biol.120 (3):725–32.doi:10.1083/jcb.120.3.725.PMC 2119537.PMID 8093886.
General
N terminus
C terminus
Single specificAAs
Serine/Threonine
Tyrosine
Cysteine
Aspartate
Glutamate
Asparagine
Glutamine
Lysine
Arginine
Proline
Histidine
Tryptophan
Crosslinks between twoAAs
CysteineCysteine
MethionineHydroxylysine
LysineTyrosine
TryptophanTryptophan
Crosslinks between threeAAs
SerineTyrosineGlycine
HistidineTyrosineGlycine
AlanineSerineGlycine
Crosslinks between fourAAs
AllysineAllysineAllysineLysine
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