Protein-coding gene in Homo sapiens
Probable ATP-dependent RNA helicase DDX5 also known asDEAD box protein 5 orRNA helicase p68 is anenzyme that in humans is encoded by theDDX5 gene .[ 5]
DEAD box proteins, characterized by the conserved motifAsp -Glu -Ala -Asp (DEAD), are putativeRNA helicases . They are implicated in a number of cellular processes involving alteration ofRNA secondary structure , such astranslation initiation , nuclear and mitochondrialsplicing , andribosome andspliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved inembryogenesis ,spermatogenesis ,[ 6] cellular growth and division. This gene encodes a DEAD box protein, which is an RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with thesimian virus 40 tumor antigen . This gene consists of 13exons , andalternatively spliced transcripts containing severalintron sequences have been detected, but no isoforms encoded by these transcripts have been identified.[ 5]
DDX5 has been shown tointeract with:
^a b c GRCh38: Ensembl release 89: ENSG00000108654 –Ensembl , May 2017^a b c GRCm38: Ensembl release 89: ENSMUSG00000020719 –Ensembl , May 2017^ "Human PubMed Reference:" .National Center for Biotechnology Information, U.S. National Library of Medicine .^ "Mouse PubMed Reference:" .National Center for Biotechnology Information, U.S. National Library of Medicine .^a b "Entrez Gene: DDX5 DEAD (Asp-Glu-Ala-Asp) box polypeptide 5" .^ Legrand J, Chan AL, La HM, Rossello FJ, Änkö ML, Fuller-Pace FV, Hobbs RM (23 May 2019)."DDX5 plays essential transcriptional and post-transcriptional roles in the maintenance and function of spermatogonia" .Nature Communications .10 (1): 2278.Bibcode :2019NatCo..10.2278L .doi :10.1038/s41467-019-09972-7 .PMC 6533336 PMID 31123254 . ^ Akileswaran L, Taraska JW, Sayer JA, Gettemy JM, Coghlan VM (May 2001)."A-kinase-anchoring protein AKAP95 is targeted to the nuclear matrix and associates with p68 RNA helicase" .J. Biol. Chem .276 (20):17448– 54.doi :10.1074/jbc.M101171200 PMID 11279182 . ^ Ogilvie VC, Wilson BJ, Nicol SM, Morrice NA, Saunders LR, Barber GN, Fuller-Pace FV (March 2003)."The highly related DEAD box RNA helicases p68 and p72 exist as heterodimers in cells" .Nucleic Acids Res .31 (5):1470– 80.doi :10.1093/nar/gkg236 .PMC 149829 PMID 12595555 . ^ Wilson BJ, Giguère V (2007)."Identification of novel pathway partners of p68 and p72 RNA helicases through Oncomine meta-analysis" .BMC Genomics .8 : 419.doi :10.1186/1471-2164-8-419 PMC 3225811 PMID 18005418 . ^ Rossow KL, Janknecht R (January 2003)."Synergism between p68 RNA helicase and the transcriptional coactivators CBP and p300" .Oncogene .22 (1):151– 6.doi :10.1038/sj.onc.1206067 PMID 12527917 . ^ Nicol SM, Causevic M, Prescott AR, Fuller-Pace FV (June 2000). "The nuclear DEAD box RNA helicase p68 interacts with the nucleolar protein fibrillarin and colocalizes specifically in nascent nucleoli during telophase".Exp. Cell Res .257 (2):272– 80.doi :10.1006/excr.2000.4886 .PMID 10837141 . ^ Wilson BJ, Bates GJ, Nicol SM, Gregory DJ, Perkins ND, Fuller-Pace FV (August 2004)."The p68 and p72 DEAD box RNA helicases interact with HDAC1 and repress transcription in a promoter-specific manner" .BMC Mol. Biol .5 : 11.doi :10.1186/1471-2199-5-11 PMC 514542 PMID 15298701 . ^ Bates GJ, Nicol SM, Wilson BJ, Jacobs AM, Bourdon JC, Wardrop J, Gregory DJ, Lane DP, Perkins ND, Fuller-Pace FV (February 2005)."The DEAD box protein p68: a novel transcriptional coactivator of the p53 tumour suppressor" .EMBO J .24 (3):543– 53.doi :10.1038/sj.emboj.7600550 .PMC 548656 PMID 15660129 . ^ Yao H, Brick K, Evrard Y, Xiao T, Camerini-Otero RD, Felsenfeld G (2010)."Mediation of CTCF transcriptional insulation by DEAD-box RNA-binding protein p68 and steroid receptor RNA activator SRA" .Genes Dev .24 (22):2543– 2555.doi :10.1101/gad.1967810 .PMC 2975930 PMID 20966046 . Iggo RD, Jamieson DJ, MacNeill SA, et al. 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Buelt MK, Glidden BJ, Storm DR (1994)."Regulation of p68 RNA helicase by calmodulin and protein kinase C" .J. Biol. Chem .269 (47):29367– 70.doi :10.1016/S0021-9258(18)43885-9 PMID 7525583 . Brody LC, Abel KJ, Castilla LH, et al. (1995)."Construction of a transcription map surrounding the BRCA1 locus of human chromosome 17" .Genomics .25 (1):238– 47.doi :10.1016/0888-7543(95)80131-5 PMID 7774924 . Rössler OG, Hloch P, Schütz N, et al. (2000)."Structure and expression of the human p68 RNA helicase gene" .Nucleic Acids Res .28 (4):932– 9.doi :10.1093/nar/28.4.932 .PMC 102575 PMID 10648785 . Nicol SM, Causevic M, Prescott AR, Fuller-Pace FV (2000). "The nuclear DEAD box RNA helicase p68 interacts with the nucleolar protein fibrillarin and colocalizes specifically in nascent nucleoli during telophase".Exp. Cell Res .257 (2):272– 80.doi :10.1006/excr.2000.4886 .PMID 10837141 . Akileswaran L, Taraska JW, Sayer JA, et al. (2001)."A-kinase-anchoring protein AKAP95 is targeted to the nuclear matrix and associates with p68 RNA helicase" .J. Biol. Chem .276 (20):17448– 54.doi :10.1074/jbc.M101171200 PMID 11279182 . Andersen JS, Lyon CE, Fox AH, et al. (2002)."Directed proteomic analysis of the human nucleolus" .Curr. Biol .12 (1):1– 11.Bibcode :2002CBio...12....1A .doi :10.1016/S0960-9822(01)00650-9 PMID 11790298 .S2CID 14132033 . Jurica MS, Licklider LJ, Gygi SR, et al. (2002)."Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis" .RNA .8 (4):426– 39.doi :10.1017/S1355838202021088 .PMC 1370266 PMID 11991638 . Liu ZR (2002)."p68 RNA Helicase Is an Essential Human Splicing Factor That Acts at the U1 snRNA-5′ Splice Site Duplex" .Mol. Cell. Biol .22 (15):5443– 50.doi :10.1128/MCB.22.15.5443-5450.2002 .PMC 133941 PMID 12101238 . Strausberg RL, Feingold EA, Grouse LH, et al. (2003)."Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences" .Proc. Natl. Acad. Sci. U.S.A .99 (26):16899– 903.Bibcode :2002PNAS...9916899M .doi :10.1073/pnas.242603899 PMC 139241 PMID 12477932 . Rossow KL, Janknecht R (2003)."Synergism between p68 RNA helicase and the transcriptional coactivators CBP and p300" .Oncogene .22 (1):151– 6.doi :10.1038/sj.onc.1206067 PMID 12527917 . Guil S, Gattoni R, Carrascal M, et al. (2003)."Roles of hnRNP A1, SR Proteins, and p68 Helicase in c-H-ras Alternative Splicing Regulation" .Mol. Cell. Biol .23 (8):2927– 41.doi :10.1128/MCB.23.8.2927-2941.2003 .PMC 152554 PMID 12665590 . Fujita T, Kobayashi Y, Wada O, et al. (2003)."Full activation of estrogen receptor alpha activation function-1 induces proliferation of breast cancer cells" .J. Biol. Chem .278 (29):26704– 14.doi :10.1074/jbc.M301031200 PMID 12738788 . Ota T, Suzuki Y, Nishikawa T, et al. (2004)."Complete sequencing and characterization of 21,243 full-length human cDNAs" .Nat. Genet .36 (1):40– 5.doi :10.1038/ng1285 PMID 14702039 .
Activity Regulation Classification Kinetics Types
