Christiane Nüsslein-Volhard in 2007A preparation of the cuticle from aDrosophila embryo, similar to those examined by Nüsslein-Volhard. Note the bands of denticles on the left hand side (towards the head) of each segment.
Nüsslein-Volhard was born inMagdeburg on 20 October 1942, the second of five children to Rolf Volhard, an architect, and Brigitte Haas Volhard, a nursery school teacher.[5] She has four siblings: three sisters and one brother. She grew up and went to school in southFrankfurt,[5] where she was exposed to art and music and thus was "trained in looking at things and recognizing things".[6] Her great-grandfather was the chemistJacob Volhard, and her grandfather was the knowninternistFranz Volhard. She is also the aunt of the Nobel laureate in chemistryBenjamin List.[7]
After theAbitur in 1962, she briefly considered pursuing medicine, but dropped the idea after doing a month-long nursing course in a hospital. Instead, she opted to study biology atGoethe University Frankfurt.[5] In 1964 Nüsslein-Volhard left Frankfurt for theUniversity of Tübingen, to start a new course in biochemistry. She originally wanted to dobehavioral biology, "but then somehow I ended up in biochemistry (...) and molecular genetics because at the time this was the most modern aspect, and I was ambitious — I wanted to go where the leaders were. The old-fashioned botanists and zoologists were such dull people— there was nothing interesting there."[6]
In 1975, Nüsslein-Volhard became a postdoctoral researcher inWalter Gehring´s laboratory at theBiozentrum, University of Basel. She was a specialist in the developmental biology ofDrosophila melanogaster (fruit fly) supported by a long-term fellowship from theEuropean Molecular Biology Organization (EMBO).In 1977, she continued in the laboratory ofKlaus Sander atUniversity of Freiburg, who was an expert in embryonic patterning.In 1978, she set up her own lab in the newly foundedEuropean Molecular Biology Laboratory in Heidelberg withEric Wieschaus, whom she had met in Basel. Over the next three years they examined about 20,000 mutated fly families, collected about 600 mutants with an altered body pattern and found that out of the approximately 5,000 essential genes only 120 were essential for early development. In October 1980, they published the mere 15 genes controlling the segmented pattern of the Drosophila larva.[5]
In 2001, she became a member of theNationaler Ethikrat (National Ethics Council of Germany) for theethical assessment of new developments in thelife sciences and their influence on the individual and society. Her primer for the lay-reader,Coming to Life: How Genes Drive Development, was published in April 2006.
In 2004, she started the Christiane Nüsslein-Volhard Foundation (Christiane Nüsslein-Volhard Stiftung) which aids promising young female German scientists with children. The foundation's main focus is to facilitate childcare as a supplement to existing stipends and day care.[4]
During the late 1970's and early 1980's, little was known about the genetic and molecular mechanisms by which multicellular organismsdevelop from single cells to morphologically complex forms duringembryogenesis. Nüsslein-Volhard and Wieschaus identified genes involved in embryonic development by a series of genetic screens, generating randommutations in fruit flies usingethyl methanesulfonate. Some of these mutations affected genes involved in the development of the embryo. They took advantage of thesegmented form ofDrosophila larvae to address the logic of the genes controlling development. They looked at the pattern of segments and denticles in each mutant under the microscope, and were therefore able to work out that particular genes were involved in different processes during development based on their differing mutant phenotypes (such as fewer segments, gaps in the normal segment pattern, and alterations in the patterns of denticles on the segments). Many of these genes were given descriptive names based on the appearance of the mutant larvae, such ashedgehog,gurken (German: "cucumbers"), andKrüppel ("cripple").[10] Later, researchers Pavel Tomancak, Amy Beaton, et al., identified exactly which gene had been affected by each mutation, thereby identifying a set of genes crucial forDrosophila embryogenesis.
The subsequent study of these mutants and their interactions led to important new insights into earlyDrosophila development, especially the mechanisms that underlie the step-wise development of body segments.[11] These experiments are not only distinguished by their sheer scale (with the methods available at the time, they involved an enormous workload), but more importantly by their significance for organisms other than fruit flies.
Her findings led to important realizations about evolution – for example, thatprotostomes anddeuterostomes are likely to have had a relatively well-developedcommon ancestor with a much more complex body plan than had been conventionally thought.
Additionally, they greatly increased our understanding of the regulation oftranscription, as well as cell fate during development.[citation needed]
Nüsslein-Volhard is associated with the discovery ofToll, which led to the identification oftoll-like receptors.[12]
Nüsslein-Volhard married in the mid-1960s while studying at theGoethe University Frankfurt, but divorced soon afterward and did not have any children.[5] She lives inBebenhausen, Germany.[14] She has said that she loves to sing, play the flute and do chamber music.[6] She published a cookbook in 2006.[15]
^Nüsslein-Volhard, Christiane (1974).Zur spezifischen Protein-Nukleinsäure-Wechselwirkung: die Bindung von RNS-Polymerase aus Escherichia coli an die Replikative-Form-DNS des Bakteriophagen fd und die Charakterisierung der Bindungsstellen (PhD thesis). University of Tübingen.OCLC793770536.
Christiane Nüsslein-Volhard on Nobelprize.org including the Nobel Lecture on 8 December 1995The Identification of Genes Controlling Development in Flies and Fishes
