A laboratory tabletop centrifuge. The rotating unit, called therotor, has fixed holes drilled at an angle (to the vertical), visible inside the smooth silver rim.Sample tubes are placed in these slots and the motor is spun. As the centrifugal force is in the horizontal plane and the tubes are fixed at an angle, the particles have to travel only a short distance before they hit the wall of the tube and then slide down to the bottom. These angle rotors are very popular in the lab for routine use.
Acentrifuge is a device that usescentrifugal force to subject a specimen to a specified constant force - for example, to separate various components of afluid. This is achieved byspinning the fluid at high speed within a container, thereby separating fluids of differentdensities (e.g. cream from milk) or liquids from solids. It works by causing denser substances and particles to move outward in the radial direction. At the same time, objects that are less dense are displaced and moved to the centre. In alaboratory centrifuge that uses sample tubes, the radial acceleration causes denser particles to settle to the bottom of the tube, while low-density substances rise to the top.[1] A centrifuge can be a very effective filter that separates contaminants from the main body of fluid.
Industrial scale centrifuges are commonly used in manufacturing and waste processing to sedimentsuspended solids, or to separateimmiscible liquids. An example is thecream separator found indairies. Very high speed centrifuges andultracentrifuges able to provide very high accelerations can separate fine particles down to the nano-scale, and molecules of different masses. Large centrifuges are used to simulate high gravity or acceleration environments (for example,high-G training for test pilots). Medium-sized centrifuges are used inwashing machines and at someswimming pools to draw water out of fabrics.Gas centrifuges are used forisotope separation, such as to enrichnuclear fuel forfissile isotopes.
A 19th-century hand-cranked laboratory centrifuge.
English military engineerBenjamin Robins (1707–1751) invented a whirling arm apparatus to determinedrag. In 1864, Antonin Prandtl proposed the idea of a dairy centrifuge to separate cream from milk.[2] The idea was subsequently put into practice by his brother, Alexander Prandtl, who made improvements to his brother's design, and exhibited a working butterfat extraction machine in 1875.[3]
A centrifuge machine can be described as a machine with a rapidly rotating container that applies centrifugal force to its contents. There are multiple types of centrifuge, which can be classified by intended use or by rotor design:
Fixed-angle centrifuges are designed to hold the sample containers at a constant angle relative to the central axis.
Swinging head (or swinging bucket) centrifuges, in contrast to fixed-angle centrifuges, have a hinge where the sample containers are attached to the central rotor. This allows all of the samples to swing outwards as the centrifuge is spun.
Continuous tubular centrifuges do not have individual sample vessels and are used for high volume applications.
Laboratory centrifuges, are general-purpose instruments of several types with distinct, but overlapping, capabilities. These include clinical centrifuges, superspeed centrifuges andpreparative ultracentrifuges.
Industrial centrifuges may otherwise be classified according to the type of separation of the high density fraction from the low density one.[citation needed]
Generally, there are two types of centrifuges: the filtration and sedimentation centrifuges. For the filtration or the so-called screen centrifuge, the drum is perforated and is inserted with a filter, for example a filter cloth, wire mesh or lot screen. The suspension flows through the filter and the drum with the perforated wall from the inside to the outside. In this way, the solid material is restrained and can be removed. The kind of removing depends on the type of centrifuge, for example manually or periodically. Common types are:
Centrifugal oil filters
Screen/scroll centrifuges (Screen centrifuges, where the centrifugal acceleration allows the liquid to pass through ascreen of some sort, through which the solids cannot go (due togranulometry larger than the screen gap or due to agglomeration))
In the centrifuges, the drum is a solid wall (not perforated). This type of centrifuge is used for the purification of a suspension. For the acceleration of the natural deposition, process of suspension the centrifuges use centrifugal force. With so-called overflow centrifuges, the suspension is drained off and the liquid is added constantly. Common types are:[8]
Separator centrifuges (Continuous liquid); common types are:[citation needed]
Decanter centrifuges, in which there is no physical separation between the solid and liquid phase, rather an acceleratedsettling due to centrifugal acceleration.
Though most modern centrifuges are electrically powered, a hand-powered variant inspired by thewhirligig has been developed for medical applications in developing countries.[9]
Many designs have been shared for free and open-source centrifuges that can bedigitally manufactured. Theopen-source hardware designs for hand-powered centrifuge for larger volumes of fluids with a radial velocity of over 1750 rpm and over 50 N of relative centrifugal force can be completely3-D printed for about $25.[10] Other open hardware designs use custom 3-D printed fixtures with inexpensive electric motors to make low-cost centrifuges (e.g. the Dremelfuge that uses aDremel power tool) orCNC cut out OpenFuge.[11][12][13][14]
A wide variety of laboratory-scale centrifuges are used in chemistry, biology, biochemistry andclinical medicine for isolating and separating suspensions and immiscible liquids. They vary widely in speed, capacity, temperature control, and other characteristics. Laboratory centrifuges often can accept a range of different fixed-angle and swinging bucket rotors able to carry different numbers of centrifuge tubes and rated for specific maximum speeds. Controls vary from simple electrical timers to programmable models able to control acceleration and deceleration rates, running speeds, and temperature regimes. Ultracentrifuges spin the rotors under vacuum, eliminating air resistance and enabling exact temperature control.Zonal rotors andcontinuous flow systems are capable of handing bulk and larger sample volumes, respectively, in a laboratory-scale instrument.[1]
Whole blood is often separated, using a centrifuge, into components for storage and transport
An application in laboratories is blood separation. Blood separates into cells and proteins (RBC, WBC, platelets, etc.) and serum.DNA preparation is another common application for pharmacogenetics and clinical diagnosis. DNA samples are purified and the DNA is prepped for separation by adding buffers and then centrifuging it for a certain amount of time. The blood waste is then removed and another buffer is added and spun inside the centrifuge again. Once the blood waste is removed and another buffer is added the pellet can be suspended and cooled. Proteins can then be removed and the entire thing can be centrifuged again and the DNA can be isolated completely. Specializedcytocentrifuges are used in medical and biological laboratories to concentrate cells for microscopic examination.[15]
Human centrifuges are exceptionally large centrifuges that test the reactions and tolerance ofpilots andastronauts to acceleration above those experienced in the Earth'sgravity.[citation needed]
The first centrifuges used for human research were used byErasmus Darwin, the grandfather ofCharles Darwin. The first large-scale human centrifuge designed for aeronautical training was created in Germany in 1933.[17]
TheUS Air Force at Brooks City Base, Texas, operates a human centrifuge while awaiting completion of the new human centrifuge in construction atWright-Patterson AFB, Ohio. The centrifuge at Brooks City Base is operated by theUnited States Air Force School of Aerospace Medicine for the purpose of training and evaluating prospective fighter pilots for high-g flight in Air Force fighter aircraft.[18]
The use of large centrifuges to simulate a feeling ofgravity has been proposed for future long-duration space missions. Exposure to thissimulated gravity would prevent or reduce thebone decalcification andmuscle atrophy that affect individuals exposed to long periods of freefall.[18][19]
Non-Human centrifuge
At the European Space Agency (ESA) technology center ESTEC (in Noordwijk, the Netherlands), an 8-metre (26 ft) diameter centrifuge is used to expose samples in fields of life sciences as well as physical sciences. ThisLarge Diameter Centrifuge (LDC)[20] began operation in 2007. Samples can be exposed to a maximum of 20 times Earth's gravity. With its four arms and six freely swinging out gondolas it is possible to expose samples with different g-levels at the same time. Gondolas can be fixed at eight different positions. Depending on their locations one could e.g. run an experiment at 5 and 10g in the same run. Each gondola can hold an experiment of a maximum 80 kilograms (180 lb). Experiments performed in this facility ranged from zebra fish, metal alloys, plasma,[21] cells,[22] liquids, Planaria,[23] Drosophila[24] or plants.
Industrial centrifugal separator is a coolant filtration system for separating particles from liquid like, grinding machining coolant. It is usually used for non-ferrous particles separation such as, silicon, glass, ceramic, and graphite etc. The filtering process does not require any consumption parts like filter bags, which saves the earth from harm.[25][26]
Geotechnical centrifuge modeling is used for physical testing of models involving soils. Centrifuge acceleration is applied to scale models to scale the gravitational acceleration and enable prototype scale stresses to be obtained in scale models. Problems such as building and bridge foundations, earth dams, tunnels, and slope stability, including effects such as blast loading and earthquake shaking.[27]
High gravity conditions generated by centrifuge are applied in the chemical industry, casting, and material synthesis.[28][29][30][31] The convection and mass transfer are greatly affected by the gravitational condition. Researchers reported that the high-gravity level can effectively affect the phase composition and morphology of the products.[28]
Insoil mechanics, centrifuges utilize centrifugal acceleration to match soil stresses in a scale model to those found in reality.
Large industrial centrifuges are commonly used inwater andwastewater treatment to drysludges. The resulting dry product is often termedcake, and the water leaving a centrifuge after most of the solids have been removed is calledcentrate.
Protocols for centrifugation typically specify the amount ofacceleration to be applied to the sample, rather than specifying arotational speed such asrevolutions per minute. This distinction is important because two rotors with different diameters running at the same rotational speed will subject samples to different accelerations. Duringcircular motion the acceleration is the product of theradius and the square of theangular velocity, and the acceleration relative to "g" is traditionally named "relative centrifugal force" (RCF). The acceleration is measured in multiples of "g" (or × "g"), the standard acceleration due togravity at the Earth's surface, adimensionless quantity given by the expression:
To avoid having to perform a mathematical calculation every time, one can findnomograms for converting RCF to rpm for a rotor of a given radius. A ruler or other straight edge lined up with the radius on one scale, and the desired RCF on another scale, will point at the correct rpm on the third scale.[32] Based on automatic rotor recognition, modern centrifuges have a button for automatic conversion from RCF to rpm and vice versa.[citation needed]
^Heidcamp, William H."Appendix F".Cell Biology Laboratory Manual. Gustavus Adolphus College. Archived fromthe original on 2 March 2012. Retrieved11 March 2012.
^Serrano, Paloma; van Loon, Jack J. W. A.; Medina, F. Javier; Herranz, Raúl (27 November 2012). "Relation Between Motility, Accelerated Aging and Gene Expression in Selected Drosophila Strains under Hypergravity Conditions".Microgravity Science and Technology.25 (1):67–72.doi:10.1007/s12217-012-9334-5.hdl:10261/99914.ISSN0938-0108.S2CID2121465.
^abYin, Xi; Chen pramodn; Zhou, Heping; Ning, Xiaoshan (August 2010). "Combustion Synthesis of Ti3SiC2/TiC Composites from Elemental Powders under High-Gravity Conditions".Journal of the American Ceramic Society.93 (8):2182–2187.doi:10.1111/j.1551-2916.2010.03714.x.
^Mesquita, R.A.; Leiva, D.R.; Yavari, A.R.; Botta Filho, W.J. (April 2007). "Microstructures and mechanical properties of bulk AlFeNd(Cu,Si) alloys obtained through centrifugal force casting".Materials Science and Engineering: A.452–453:161–169.doi:10.1016/j.msea.2006.10.082.
.jpg)
_(9759061442).jpg)
