CUB domain-containing protein 1 (CDCP1) is aprotein that in humans is encoded by theCDCP1gene.[5][6] CDCP1 has also been designated asCD318 (cluster of differentiation 318) andTrask (Transmembrane and associated with src kinases).Alternatively spliced transcript variants encoding distinctisoforms have been reported.[6]
CDCP1 is a 140 kD transmembraneglycoprotein with a large extracellular domain (ECD) containing twoCUB domains, and a smaller intracellular domain (ICD). CDCP1 is cleaved by serine proteases at the extracellular domain next to Arg368 to generate a truncated molecule of 80 kDa size.[7] Different cell lines express different amounts of p140 and p80, depending on the activity of endogenous serine proteases.
The intracellular domain of CDCP1 contains five tyrosine residues - Y707, Y734, Y743, Y762 and Y806. The tyrosine phosphorylation of CDCP1 in cultured cells occurs when cells are induced to detach bytrypsin orEDTA, or seen spontaneously duringmitotic detachment.
^"Human PubMed Reference:".National Center for Biotechnology Information, U.S. National Library of Medicine.
^"Mouse PubMed Reference:".National Center for Biotechnology Information, U.S. National Library of Medicine.
^Scherl-Mostageer M, Sommergruber W, Abseher R, Hauptmann R, Ambros P, Schweifer N (July 2001). "Identification of a novel gene, CDCP1, overexpressed in human colorectal cancer".Oncogene.20 (32):4402–8.doi:10.1038/sj.onc.1204566.PMID11466621.S2CID19987585.
Bühring HJ, Kuçi S, Conze T, Rathke G, Bartolović K, Grünebach F, Scherl-Mostageer M, Brümmendorf TH, Schweifer N, Lammers R (2005). "CDCP1 identifies a broad spectrum of normal and malignant stem/progenitor cell subsets of hematopoietic and nonhematopoietic origin".Stem Cells.22 (3):334–43.doi:10.1634/stemcells.22-3-334.PMID15153610.S2CID11997172.
André M, Le Caer JP, Greco C, Planchon S, El Nemer W, Boucheix C, Rubinstein E, Chamot-Rooke J, Le Naour F (March 2006). "Proteomic analysis of the tetraspanin web using LC-ESI-MS/MS and MALDI-FTICR-MS".Proteomics.6 (5):1437–49.doi:10.1002/pmic.200500180.PMID16404722.S2CID46542257.
Kimura H, Morii E, Ikeda JI, Ezoe S, Xu JX, Nakamichi N, Tomita Y, Shibayama H, Kanakura Y, Aozasa K (September 2006). "Role of DNA methylation for expression of novel stem cell marker CDCP1 in hematopoietic cells".Leukemia.20 (9):1551–6.doi:10.1038/sj.leu.2404312.PMID16926850.S2CID6809573.
