Protein-coding gene in the species Homo sapiens
Chromosome 16 open reading frame 58, orC16orf58, also known asFLJ13638 is aprotein which in humans is encoded by theC16orf58gene.[5] The gene itself is 18892 bp long, withmRNA of 2760 bp, and a protein sequence of 468amino acids. There is a conserved domain of unknown, DUF647. No function has been determined for this gene yet, but it is predicted that it resides in theendoplasmic reticulum in thecytoplasm.[6]
Species distribution
[edit]C16orf58 has very interesting conservation in that it hasorthologs back throughplants andfungi. However, it has not been found inreptiles,birds, oramphibians. The below table shows some, but not all, orthologs which were found using BLAST.[7]
Protein Interactions
[edit]Though the function is still unknown,C16orf58 has been shown to interact with three different proteins:
Although there are several sites that will give predictions on protein structure, C16orf58 does not have a known structure yet. That being said there is at least onetransmembrane domain, if not more. Within the protein structure there are several extended areas with uncharged amino acids, these could be possible transmembrane domains, orhydrophobic cores.[6] The below shows the charge of each of the amino acids in the protein sequence, + for positive, - for negative and 0 for uncharged. Note the large segments of uncharged amino acids appear bolded. These stretches of uncharged amino acids are conserved back through distant orthologs.
1 00—000-00 000-00000- 0+00+000-0 0000-0000+ 00000+0000 +0-0+-00-0 610000000000 0000000000 000-0000-0 000000-000 0000000000 0000000000 1210000+00000 0000000+-0 00000+0000 00+00+0-00 0+00+000-0 00-00000-0 1810000000000 000000000+ 0000000000 +00000000+ +0000-000+ -000-00000 2410000000000 0000000000 0000000000 000000+00+ 0000-000-0 +0+000+000 301 0+0-00-000 00+0-00000 0000000000 0000+00000 0-00000-00 0-000000-0 361 0000000000 0+000+000+ 0000000000 000-00000- 0—0+0+0+0 00++-00000 421 +-00-00-00 00+00+000- 000+0-+000 -00-0+0000 000-++00
