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Auramine–rhodamine stain

From Wikipedia, the free encyclopedia
Histological technique
Oocysts ofCryptosporidium parvum stained with the fluorescent auramine–rhodamine stain.

Theauramine–rhodamine stain (AR), also known as theTruant auramine–rhodamine stain, is ahistological technique used to visualizeacid-fast bacilli usingfluorescence microscopy, notably species in theMycobacteriumgenus.[1] Acid-fast organisms display a reddish-yellowfluorescence.[2] Although the auramine–rhodaminestain is not asspecific for acid-fast organisms (e.g.Mycobacterium tuberculosis orNocardia) as theZiehl–Neelsen stain, it is more affordable and more sensitive, therefore it is often utilized as ascreening tool.

AR stain is a mixture ofauramine O andrhodamine B. It iscarcinogenic.

See also

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References

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  1. ^Kommareddi S.; Abramowsky C.; Swinehart G.; Hrabak L. (1984). "Nontuberculous mycobacterial infections: comparison of the fluorescent auramine-O and Ziehl–Neelsen techniques in tissue diagnosis".Human Pathology.15 (11):1085–1089.doi:10.1016/S0046-8177(84)80253-1.PMID 6208117.
  2. ^Abe, C. (August 2003).結核とそれらの上達テストの実験室テストの標準化 [Standardization of laboratory tests for tuberculosis and their proficiency testing].Kekkaku (in Japanese).78 (8):541–551.ISSN 0022-9776.PMID 14509226.
Iron/hemosiderin
Lipids
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