Shikimate kinase (EC2.7.1.71) is an enzyme that catalyzes theATP-dependentphosphorylation ofshikimate to form shikimate 3-phosphate.[1] This reaction is the fifth step of the shikimate pathway,[2] which is used by plants and bacteria to synthesize the common precursor of aromatic amino acids and secondary metabolites. Thesystematic name of this enzyme class isATP:shikimate 3-phosphotransferase. Other names in common use includeshikimate kinase (phosphorylating), andshikimate kinase II.
| Shikimate kinase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
 Shikimate kinase ofErwinia chrysanthemi | |||||||||
| Identifiers | |||||||||
| Symbol | SKI | ||||||||
| Pfam | PF01202 | ||||||||
| Pfam clan | CL0023 | ||||||||
| InterPro | IPR000623 | ||||||||
| PROSITE | PDOC00868 | ||||||||
| SCOP2 | 2shk /SCOPe /SUPFAM | ||||||||
| OPM superfamily | 124 | ||||||||
| OPM protein | 1e6c | ||||||||
| |||||||||
| shikimate kinase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
 A cartoon representation of shikimate kinase fromMycobacterium tuberculosis. α-Helices are shown in red, the central β-sheet in yellow, and loops in green | |||||||||
| Identifiers | |||||||||
| EC no. | 2.7.1.71 | ||||||||
| CAS no. | 9031-51-0 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDBPDBePDBsum | ||||||||
| Gene Ontology | AmiGO /QuickGO | ||||||||
| |||||||||
Background
editThe shikimate pathway consists of seven enzymatic reactions by whichphosphoenolpyruvate anderythrose 4-phosphate are converted tochorismate, the common precursor of the aromatic amino acidsphenylalanine,tyrosine, andtryptophan. The aromatic amino acids are used in the synthesis of proteins and, in plants, fungi, and bacteria, give rise to a number of other specialized metabolites, such asphenylpropanoids andalkaloids. Chorismate and several other intermediates of the pathway serve as precursors for a number of other metabolites, such as folates, quinates, and quinones. The four enzymes that precede shikimate kinase in the pathway areDAHP synthase,3-dehydroquinate synthase,3-dehydroquinate dehydratase, andshikimate dehydrogenase, and the two that follow it areEPSP synthase andchorismate synthase. In fungi and protists, it is part of theAROM complex, in which the five central steps of the shikimate pathway are co-localized.[3] The pathway is not found in humans and other animals, which must obtain the aromatic amino acids from their food.
Activity
editThe reaction catalyzed by shikimate kinase is shown below:
This reaction involves the transfer of a phosphate group from ATP to the 3-hydroxyl group of shikimate. Shikimate kinase thus has twosubstrates,shikimate andATP, and twoproducts,shikimate 3-phosphate andADP.[4]
Examples
editHuman proteins containing this domain include:MAPK7 andTHNSL1
References
edit- ^Morell H, Sprinson DB (February 1968)."Shikimate kinase isoenzymes in Salmonella typhimurium".The Journal of Biological Chemistry.243 (3):676–7.doi:10.1016/S0021-9258(18)93656-2.PMID 4866525.
- ^Herrmann KM, Weaver LM (June 1999). "The Shikimate Pathway".Annual Review of Plant Physiology and Plant Molecular Biology.50:473–503.doi:10.1146/annurev.arplant.50.1.473.PMID 15012217.
- ^Arora Verasztó H, Logotheti M, Albrecht R, Leitner A, Zhu H, Hartmann MD (July 2020). "Architecture and functional dynamics of the pentafunctional AROM complex".Nature Chemical Biology.16 (9):973–978.doi:10.1038/s41589-020-0587-9.PMID 32632294.S2CID 220375879.
- ^Hartmann MD, Bourenkov GP, Oberschall A, Strizhov N, Bartunik HD (December 2006). "Mechanism of phosphoryl transfer catalyzed by shikimate kinase from Mycobacterium tuberculosis".Journal of Molecular Biology.364 (3):411–23.doi:10.1016/j.jmb.2006.09.001.PMID 17020768.
