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Immunohistochemical Staining of Fixed Tissues

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Part of the book series:Methods in Molecular Biology™ ((MIMB,volume 104))

Abstract

Immunohistochemistry is a technique in which the specific interaction between an immunoglobulin and its homologous antigen is visualized on histological sections by a microscopically detectable label. Generally, the label consists of an enzyme, such as peroxidase, alkaline phosphatase, or glucose oxidase that reacts with an appropriate substrate-chromogen solution to produce a specific color at the site of reaction. Several immunohistochemical techniques have been developed and the most important are schematically represented inFig. 1. In the direct method, the primary antibody is directly labeled with the enzyme. In the indirect method, an enzyme-labeled secondary antibody is directed against the immunoglobulin type of the animal species in which the primary antibody has been raised. Both methods have a relatively low sensitivity and are therefore not frequently used.

Schematic representation of immunohistochemical techniques. Direct method, indirect method, ([A] left to right) PAP complex procedure, ABC procedure ([B] left to right). A=avidin; B=biotin; C=substrate-chromogen; P=peroxidase.

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Author information

Authors and Affiliations

  1. Istituto di Anatomia Patologica Veterinaria e Patologica Aviare, Milano, Italy

    Eugenio Scanziani

Authors
  1. Eugenio Scanziani

Editor information

Editors and Affiliations

  1. King’s College, London, England

    Roger Miles

  2. Central Veterinary Laboratory, Surrey, England

    Robin Nicholas

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© 1998 Humana Press Inc., Totowa, NJ

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Scanziani, E. (1998). Immunohistochemical Staining of Fixed Tissues. In: Miles, R., Nicholas, R. (eds) Mycoplasma Protocols. Methods in Molecular Biology™, vol 104. Humana Press. https://doi.org/10.1385/0-89603-525-5:133

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