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medRxiv

GENOMIC CHARACTERISATION AND PHYLOGENETIC ANALYSIS OF SARS-COV-2 IN ITALY

GianguglielmoZehender,AlessiaLai,AnnalisaBergna,LucaMeroni,AgostinoRiva,ClaudiaBalotta,MaciejTarkowski,AriannaGabrieli,DarioBernacchia,StefanoRusconi,GiulianoRizzardini,SpinelloAntinori,MassimoGalli
doi: https://doi.org/10.1101/2020.03.15.20032870
Gianguglielmo Zehender
1Department of Biomedical and Clinical Sciences Luigi Sacco, University of Milan, Milan,Italy
2EpiSoMi CRC-Coordinated Research Center, University of Milan, Milan,Italy
3Romeo ed Enrica Invernizzi Pediatric Research Center, University of Milan, Milan,Italy
Alessia Lai
1Department of Biomedical and Clinical Sciences Luigi Sacco, University of Milan, Milan,Italy
2EpiSoMi CRC-Coordinated Research Center, University of Milan, Milan,Italy
Annalisa Bergna
1Department of Biomedical and Clinical Sciences Luigi Sacco, University of Milan, Milan,Italy
Luca Meroni
4III Division of Infectious Diseases, ASST Fatebenefratelli Sacco, Luigi Sacco Hospital, Milan,Italy
Agostino Riva
4III Division of Infectious Diseases, ASST Fatebenefratelli Sacco, Luigi Sacco Hospital, Milan,Italy
Claudia Balotta
1Department of Biomedical and Clinical Sciences Luigi Sacco, University of Milan, Milan,Italy
Maciej Tarkowski
1Department of Biomedical and Clinical Sciences Luigi Sacco, University of Milan, Milan,Italy
Arianna Gabrieli
1Department of Biomedical and Clinical Sciences Luigi Sacco, University of Milan, Milan,Italy
Dario Bernacchia
4III Division of Infectious Diseases, ASST Fatebenefratelli Sacco, Luigi Sacco Hospital, Milan,Italy
Stefano Rusconi
1Department of Biomedical and Clinical Sciences Luigi Sacco, University of Milan, Milan,Italy
4III Division of Infectious Diseases, ASST Fatebenefratelli Sacco, Luigi Sacco Hospital, Milan,Italy
Giuliano Rizzardini
5Department of Infectious Diseases, Luigi Sacco Hospital, Milan,Italy
Spinello Antinori
1Department of Biomedical and Clinical Sciences Luigi Sacco, University of Milan, Milan,Italy
4III Division of Infectious Diseases, ASST Fatebenefratelli Sacco, Luigi Sacco Hospital, Milan,Italy
Massimo Galli
1Department of Biomedical and Clinical Sciences Luigi Sacco, University of Milan, Milan,Italy
2EpiSoMi CRC-Coordinated Research Center, University of Milan, Milan,Italy
4III Division of Infectious Diseases, ASST Fatebenefratelli Sacco, Luigi Sacco Hospital, Milan,Italy
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ABSTRACT

This report describes the isolation, the molecular characterization and the phylogenetic analysis of the first three complete genomes of SARS-CoV-2 isolated from three patients involved in the first outbreak of COVID-19 in Lombardy, Italy. Early molecular epidemiological tracing suggests that SARS-CoV-2 was present in Italy weeks before the first reported cases of infection.

Competing Interest Statement

The authors have declared no competing interest.

Funding Statement

None

Author Declarations

All relevant ethical guidelines have been followed; any necessary IRB and/or ethics committee approvals have been obtained and details of the IRB/oversight body are included in the manuscript.

Yes

All necessary patient/participant consent has been obtained and the appropriate institutional forms have been archived.

Yes

I understand that all clinical trials and any other prospective interventional studies must be registered with an ICMJE-approved registry, such as ClinicalTrials.gov. I confirm that any such study reported in the manuscript has been registered and the trial registration ID is provided (note: if posting a prospective study registered retrospectively, please provide a statement in the trial ID field explaining why the study was not registered in advance).

Yes

I have followed all appropriate research reporting guidelines and uploaded the relevant EQUATOR Network research reporting checklist(s) and other pertinent material as supplementary files, if applicable.

Yes

Data Availability

A paper have been submitted to Journal of Medical Virology.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license.
Posted March 20, 2020.
GENOMIC CHARACTERISATION AND PHYLOGENETIC ANALYSIS OF SARS-COV-2 IN ITALY
GianguglielmoZehender,AlessiaLai,AnnalisaBergna,LucaMeroni,AgostinoRiva,ClaudiaBalotta,MaciejTarkowski,AriannaGabrieli,DarioBernacchia,StefanoRusconi,GiulianoRizzardini,SpinelloAntinori,MassimoGalli
medRxiv2020.03.15.20032870;doi: https://doi.org/10.1101/2020.03.15.20032870
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