Abstract
MicroRNAs have been discovered as regulators of gene expression and thus their potential in clinical diseasediagnostics, prognosis and therapy is being actively pursued. MicroRNAs play an important role in atherosclerosis-relateddiseases, such as cerebrovascular and cardiovascular disease. However, the effect of miR-185 and miR-146a on patientswith ischemic stroke (IS) in the different phases has not been reported. In this study, we investigated the amounts of threemiRNAs, miR-185, miR-146a, and miR-145 in blood circulation of IS patients. We enrolled 60 patients with IS in theacute or sub-acute phase and 30 healthy controls. We divided the patients into two groups, patients with ischemic stroke inthe acute phase (ISA) and patients with ischemic stroke in the subacute phase (ISS). We measured circulating miRNAsexpression by miRNA microarray and real-time polymerase chain reaction (PCR) analysis. Testing by miRNA microarrayand RT-PCR analyses showed that miR-145 levels in healthy subjects were similar to patients with IS, whereas miR-146aand miR-185 were present with quite low abundance in ISA compared with healthy individuals; moreover, we found thatmiR-146a levels were downregulated in ISA but upregulated in ISS which may help provide new insights into the diagnosisand therapy of IS.
Keywords:MicroRNAs, blood, MiR-185, MiR-146a, ischemic stroke, phase.
Current Neurovascular Research
Title:Differential Regulation of microRNAs in Patients with Ischemic Stroke
Volume: 12Issue: 3
Author(s):Sheng-Hua Li, Sheng-You Su and Jing-Li Liu
Affiliation:
Keywords:MicroRNAs, blood, MiR-185, MiR-146a, ischemic stroke, phase.
Abstract: MicroRNAs have been discovered as regulators of gene expression and thus their potential in clinical diseasediagnostics, prognosis and therapy is being actively pursued. MicroRNAs play an important role in atherosclerosis-relateddiseases, such as cerebrovascular and cardiovascular disease. However, the effect of miR-185 and miR-146a on patientswith ischemic stroke (IS) in the different phases has not been reported. In this study, we investigated the amounts of threemiRNAs, miR-185, miR-146a, and miR-145 in blood circulation of IS patients. We enrolled 60 patients with IS in theacute or sub-acute phase and 30 healthy controls. We divided the patients into two groups, patients with ischemic stroke inthe acute phase (ISA) and patients with ischemic stroke in the subacute phase (ISS). We measured circulating miRNAsexpression by miRNA microarray and real-time polymerase chain reaction (PCR) analysis. Testing by miRNA microarrayand RT-PCR analyses showed that miR-145 levels in healthy subjects were similar to patients with IS, whereas miR-146aand miR-185 were present with quite low abundance in ISA compared with healthy individuals; moreover, we found thatmiR-146a levels were downregulated in ISA but upregulated in ISS which may help provide new insights into the diagnosisand therapy of IS.
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Cite this article as:
Li Sheng-Hua, Su Sheng-You and Liu Jing-Li, Differential Regulation of microRNAs in Patients with Ischemic Stroke, Current Neurovascular Research 2015; 12 (3) .https://dx.doi.org/10.2174/1567202612666150605121709
| DOI https://dx.doi.org/10.2174/1567202612666150605121709 | Print ISSN 1567-2026 |
| Publisher Name Bentham Science Publisher | Online ISSN 1875-5739 |
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