Abstract
Background: Hepatocellular carcinoma (HCC) has high mortality partly due to acquiring drug resistance during chemotherapytreatment. Therefore, it is necessary to explore the underlying mechanism of drug resistance.
Methods: We used 2-DE and MALDI-TOF-MS analysis to explore the possible molecular insight into 5-FU resistance in HCC. The differentiallyexpressed proteins were validated by Western blot analysis.
Results: We identified 102 unique proteins including p16, maspin, PRDX6, PSMB7, MYL6, PHB, and HSP27 with alteration in SMMC-7721/5-FU. Furthermore, down-regulation of PRDX6 and PSMB7 enhanced SMMC-7721/5-FU cells to 5-FU sensitivity.
Conclusions: Our study suggests that targeting drug resistant genes such as PRDX6 and PSMB7 could be a novel approach to overcome5-FU resistance in HCC cells.
Keywords:Hepatocellular carcinoma, chemoresistance, 5-FU, PRDX6, PMSB7.
Current Pharmaceutical Design
Title:Proteomic-based Analysis for Identification of Proteins Involved in 5-fluorouracil Resistance in Hepatocellular Carcinoma
Volume: 20Issue: 1
Author(s):Yi Tan, Shukui Qin, Xin Hou, Xiujuan Qian, Jun Xia, Yumei Li, Rui Wang, Changjie Chen, Qingling Yang, Lucio Miele, Qiong Wu and Zhiwei Wang
Affiliation:
Keywords:Hepatocellular carcinoma, chemoresistance, 5-FU, PRDX6, PMSB7.
Abstract: Background: Hepatocellular carcinoma (HCC) has high mortality partly due to acquiring drug resistance during chemotherapytreatment. Therefore, it is necessary to explore the underlying mechanism of drug resistance.
Methods: We used 2-DE and MALDI-TOF-MS analysis to explore the possible molecular insight into 5-FU resistance in HCC. The differentiallyexpressed proteins were validated by Western blot analysis.
Results: We identified 102 unique proteins including p16, maspin, PRDX6, PSMB7, MYL6, PHB, and HSP27 with alteration in SMMC-7721/5-FU. Furthermore, down-regulation of PRDX6 and PSMB7 enhanced SMMC-7721/5-FU cells to 5-FU sensitivity.
Conclusions: Our study suggests that targeting drug resistant genes such as PRDX6 and PSMB7 could be a novel approach to overcome5-FU resistance in HCC cells.
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Cite this article as:
Tan Yi, Qin Shukui, Hou Xin, Qian Xiujuan, Xia Jun, Li Yumei, Wang Rui, Chen Changjie, Yang Qingling, Miele Lucio, Wu Qiong and Wang Zhiwei, Proteomic-based Analysis for Identification of Proteins Involved in 5-fluorouracil Resistance in Hepatocellular Carcinoma, Current Pharmaceutical Design 2014; 20 (1) .https://dx.doi.org/10.2174/138161282001140113125143
| DOI https://dx.doi.org/10.2174/138161282001140113125143 | Print ISSN 1381-6128 |
| Publisher Name Bentham Science Publisher | Online ISSN 1873-4286 |
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