Part of the book series:Methods in Molecular Biology™ ((MIMB,volume 125))
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Abstract
Although it is possible to determine and characterize the total carbohydrate profile after release of the mucin oligosaccharides (usually by β-elimination), it is challenging to assign the sites of glycosylation (macroheterogeneity) and the carbohydrate heterogeneity at a given glycosylation site (microheterogeneity). Typically, the characterization of macro- and microheterogeneity has been dependent on the isolation of small peptides with only one glycosylation site. However, this is not possible with high molecular weight, heavily glycosylated domains such as those found in mucins.
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Authors and Affiliations
Macquarie University Centre for Analytical Biotechnology, School of Biological Sciences, Macquarie University, Sydney, Australia
Natasha E. Zachara
Proteome Systems Ltd., Sydney, New South Wales, Australia
Andrew A. Gooley
- Natasha E. Zachara
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- Andrew A. Gooley
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Editors and Affiliations
Bristol Royal Infirmary, Bristol, UK
Anthony P. Corfield
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© 2000 Humana Press Inc.
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Zachara, N.E., Gooley, A.A. (2000). Identification of Glycosylation Sites in Mucin Peptides by Edman Degradation. In: Corfield, A.P. (eds) Glycoprotein Methods and Protocols. Methods in Molecular Biology™, vol 125. Humana Press. https://doi.org/10.1385/1-59259-048-9:121
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