Part of the book series:Methods in Molecular Biology™ ((MIMB,volume 360))
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Summary
Transgenic animals have become a key tool in functional genomics to generate models for human diseases and validate new drugs. Transgenesis includes the addition of foreign genetic information to animals and specific inhibition of endogenous gene expression. Recently, animal models provided novel insight and significantly improved our understanding of the initiation and perpetuation of human diseases. Moreover, they are an invaluable tool for target discovery, validation, and production of therapeutic proteins. However, despite the generation of several transgenic and knockout models, obtaining relevant models still faces several theoretical and technical challenges. Indeed, genes of interest are not always available and gene addition or inactivation sometimes does not allow clear conclusions because of the intrinsic complexity of living organisms or the redundancy of some metabolic pathways. In addition to homologous recombination, endogenous gene expression can be specifically inhibited using several mechanisms such as RNA interference. Here, some animal models are described to illustrate their importance in biomedical research. Moreover, guidelines for generation of these animals are presented.
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References
Brown, S. D. and Balling, R. (2001) Systematic approaches to mouse mutagenesis.Curr. Opin. Genet. Dev.11, 268–273.
Houdebine, L. M. (2004) Preparation of recombinant proteins in milk.Methods Mol. Biol.267, 485–494.
Houdebine, L. M. and Weill, B. (1999) The impact of transgenesis and cloning on cell and organ xenotransplantation to humans, inFocus on Biotechnology (Van Brockhoven, A., Shapiro, F., and Anne, J., eds.), Kluwer Academic Publishers, pp. 351–361.
Houdebine, L. M. (2002) Transgenesis to improve animal production.Livest. Prod. Sci.74, 255–268.
Houdebine, L. M. (ed.) (1997)Transgenic Animals. Generation and Use. Harwood Academic Publishers, Amsterdam.
Pinkert, C. A. (2002)Transgenic Animal Technology. Academic, Orlando, FL.
Schedl, A., Larin, Z., Montoliu, L., et al. (1993) A method for the generation of YAC transgenic mice by pronuclear microinjection.Nucleic Acids Res.21, 4783–4787.
Hostetler, H. A., Peck, S. L., and Muir, W. M. (2003) High efficiency production of germ-line transgenic Japanese medaka (Oryzias latipes) by electroporation with direct current-shifted radio frequency pulses.Transgenic Res.12, 413–424.
Dupuy, A. J., Clark, K., Carlson, C. M., et al. (2002) Mammalian germ-line transgenesis by transposition.Proc. Natl. Acad. Sci. USA99, 4495–4499.
Tamura, T., Thibert, C., Royer, C., et al. (1999) Germiline transformation of the silkwormBombyx mori L. using a piggyBac transposon derived vector.Nat. Biotechnol.18, 81–84.
Mikkelsen, J. G., Yant, S. R., Meuse, L., Huang, Z., Xu, H., and Kay, M. A. (2003) Helper-independent Sleeping Beauty transposon-transposase vectors for efficient nonviral gene delivery and persistent gene expressionin vivo. Mol. Ther.8, 654–665.
Masuda, K., Yamamoto, S., Endoh, M., and Kaneda, Y. (2004) Transposon-independent increase of transcription by the Sleeping Beauty transposase.Biochem. Biophys. Res. Commun.317, 796–800.
Lois, C., Hong, E. J., Pease, S., Brown, E. J., and Baltimore, D. (2002) Germline transmission and tissue-specific expression of transgenes delivered by lentiviral vectors.Science295, 868–872.
Chan, A. W., Chong, K. Y., Martinovich, C., Simerly, C., and Schatten, G. (2001) Transgenic monkeys produced by retroviral gene transfer into mature oocytes.Science291, 309–312.
McGrew, M. J., Sherman, A., Ellard, F. M., et al. (2004) Efficient production of germline transgenic chickens using lentiviral vectors.EMBO Rep.5, 728–733.
Hofmann, A., Kessler, B., Ewerling, S., et al. (2003) Efficient transgenesis in farm animals by lentiviral vectors.EMBO Rep.4, 1054–1060.
Whitelaw, C. B. A. (2004) Transgenic livestock made easy.Trends Biotechnol.22, 157–159.
Hofmann, A., Zakhartchenko, V., Weppert, M., et al. (2004) Generation of transgenic cattle by lentiviral gene transfer into oocytes.Biol. Reprod.71, 405–409.
Fassler, R. (2004) Lentiviral transgene vectors.EMBO Rep.5, 28–29.
Pfeifer, A., Hofmann, A., Kessler, B., and Wolf, E. (2004) Response to Whitelaw: Lentiviral transgenesis in livestock.Trends Biotechnol.22, 159–160.
Wolfgang, M. J., Eisele, S. G., Browne, M. A., et al. (2001) Rhesus monkey placental transgene expression after lentiviral gene transfer into preimplantation embryos.Proc. Natl. Acad. Sci. USA98, 10,728–10,732.
Sanchez, O., Toledo, J. R., Rodriguez, M. P., and Castro, F. O. (2004) Adenoviral vector mediates high expression levels of human growth hormone in the milk of mice and goats.J. Biotechnol.114, 89–97.
Lipps, H. J., Jenke, A. C., Nehlsen, K., Scinteie, M. F., Stehle, I. M., and Bode, J. (2003) Chromosome-based vectors for gene therapy.Gene304, 23–33.
Lindenbaum, M., Perkins, E., Csonka, E., et al. (2004) A mammalian artificial chromosome engineering system (ACE System) applicable to biopharmaceutical protein production, transgenesis and gene-based cell therapy.Nucleic Acids Res.32, e172.
Kuroiwa, Y., Kasinathan, P., Choi, Y. J., et al. (2002) Cloned transchromosomic calves producing human immunoglobulin.Nat. Biotechnol.20, 889–894.
Lavitrano, M., Bacci, M. L., Forni, M., et al. (2002) Efficient production by sperm-mediated gene transfer of human decay accelerating factor (hDAF) transgenic pigs for xenotransplantation.Proc. Natl. Acad. Sci. USA99, 14,230–14,235.
Lavitrano, M., Forni, M., Bacci, M. L., et al. (2003) Sperm mediated gene transfer in pig: Selection of donor boars and optimization of DNA uptake.Mol. Reprod. Dev.64, 284–291.
Wang, H. J., Lin, A. X., Zhang, Z. C., and Chen, Y. F. (2001) Expression of porcine growth hormone gene in transgenic rabbits as reported by green fluorescent protein.Anim. Biotechnol.12, 101–110.
Wang, K. (2003) Improving sperm mediated transgenesis: linker based sperm gene transfer: application to multiple species with a high success rate, in Proceedings of the Transgenic Animal Research Conference IV, Tahoe City, CA.
Marsh-Armstrong, N., Huang, H., Berry, D. L., and Brown, D. D. (1999) Germline transmission of transgenes inXenopus laevis. Proc. Natl. Acad. Sci. USA96, 14,389–14,393.
Kato, M., Ishikawa, A., Kaneko, R., Yagi, T., Hochi, S., and Hirabayashi, M. (2004) Production of transgenic rats by ooplasmic injection of spermatogenic cells exposed to exogenous DNA: a preli Carl A. Pinkert Ed. (Ed.)minary study.Mol. Reprod. Dev.69, 153–158.
Moreira, P. N., Giraldo, P., Cozar, P., et al. (2004) Efficient generation of transgenic mice with intact yeast artificial chromosomes by intracytoplasmic sperm injection.Biol. Reprod.71, 1943–1947.
Chan, A. W., Luetjens, C. M., Dominko, T., et al. (2000) Transgene ICSI reviewed: Foreign DNA transmission by intracytoplasmic sperm injection in rhesus monkey.Mol. Reprod. Dev.56, 325–328.
Thermes, V., Grabher, C., Ristoratore, F., et al. (2002) I-SceI meganuclease mediates highly efficient transgenesis in fish.Mech. Dev.118, 91–98.
Chang, K., Qian, J., Jiang, M., et al. (2002) Effective generation of transgenic pigs and mice by linker based sperm-mediated gene transfer.BMC Biotechnol.2, 5.
Wang, K. (2003) Improving sperm mediated transgenesis: linker based sperm gene transfer: application to multiple species with a high success rate, in Proceedings of the Transgenic Animal Research Conference IV, Tahoe City, CA.
Celebi, C., Auvray, P., Benvegnu, T., Plusquellec, D., Jegou, B., and Guillaudeux, T. (2002) Transient transmission of a transgene in mouse offspring followingin vivo transfection of male germ cells.Mol. Reprod. Dev.62, 477–482.
Honaramooz, A., Behboodi, E., Blash, S., Megee, S. O., and Dobrinski, I. (2003) Germ cell transplantation in goats.Mol. Reprod. Dev.64, 422–428.
Readhead, C., Jarvis, S., Morgan, D., and Winston, R. (2003) Male germ cells: manipulating their genome, in Proceedings of the Transgenic Animal Research Conference IV, Tahoe City, CA.
Oatley, J. M., de Avila, D. M., Reeves, J. J., and McLean, D. J. (2004) Spermatogenesis and germ cell transgene expression in xenografted bovine testicular tissue.Biol. Reprod.71, 494–501.
Schnieke, A. E., Kind, A. J., Ritchie, W. A., et al. (1997) Human factor IX transgenic sheep produced by transfer of nuclei from transfected fetal fibroblasts.Science278, 2130–2133.
Cibelli, J. B., Stice, S. L., Golueke, P. J., et al. (1998) Transgenic bovine chimeric offspring produced from somatic cell-derived stem-like cells.Nat. Biotechnol.16, 642–646.
Capecchi, M. R. (1989) Altering the genome by homologous recombination.Science244, 1288–1292.
Cohen-Tannoudji, M., Robine, S., Choulika, A., et al. (1998) I-SceI-induced gene replacement at a natural locus in embryonic stem cells.Mol. Cell Biol.18, 1444–1448.
Epinat, J. C., Arnould, S., Chames, P., et al. (2003) A novel engineered meganuclease induces homologous recombination in yeast and mammalian cells.Nucleic Acids Res.31, 2952–2962.
Farhadi, H. F., Lepage, P., Forghani, R., et al. (2003) A combinatorial network of evolutionarily conserved myelin basic protein regulatory sequences confers distinct glial-specific phenotypes.J. Neurosci.23, 10,214–10,223.
Bode, J., Schlake, T., Iber, M., et al. (2000) The transgeneticist’s toolbox: novel methods for the targeted modification of eukaryotic genomes.Biol. Chem.381, 801–813.
Baer, A. and Bode, J. (2001) Coping with kinetic and thermodynamic barriers: RMCE, an efficient strategy for the targeted integration of transgenes.Curr. Opi. Biotechnol.12, 473–480.
Houdebine, L. M. (2003)Animal Trangenesis and Cloning. Wiley, Chichester, U.K.
West, A. G., Gaszner, M., and Felsenfeld, G. (2002) Insulators; many functions, many mechanisms.Genes Dev.16, 271–288.
Bell, A. C., West, A. G., and Felsenfeld, G. (2001) Insulators and boundaries: versatile regulatory elements in the eukaryotic genome.Science291, 447–450.
De Laat, W. and Grosveld, F. (2003) Spatial organization of gene expression: the active chromatin hub.Chromosome Res.11, 447–459.
Taboit-Dameron, F., Malassagne, B., Viglietta, C., et al. (1999) Association of the 5′HS4 sequence of the chicken beta-globin locus control region with human EF1 alpha gene promoter induces ubiquitous and high expression of human CD55 and CD59 cDNAs in transgenic rabbits.Transgenic Res.8, 223–235.
Rival-Gervier, S., Viglietta, C., Maeder, C., Attal, J., and Houdebine, L. M. (2002) Position-independent and tissue-specific expression of porcine whey acidic protein gene from a bacterial artificial chromosome in transgenic mice.Mol. Reprod. Dev.63, 161–167.
Giraldo, P., Rival-Gervier, S., Houdebine, L. M., and Montoliu, L. (2003) The potential benefits of insulators on heterologous constructs in transgenic animals.Transgenic Res.12, 751–755.
Zhang, Y., Muyrers, J. P., Testa, G., and Stewart, A. F. (2000) DNA cloning by homologous recombination inEscherichia coli. Nat. Biotechnol.18, 1314–1317.
Houdebine, L., Attal, J., and Vilotte, J. L. (2002) Vector design for transgene expression, inTransgenic Animal Technology, 2nd ed., Pinkert, C. A. (ed.), pp. 419–458.
Cohen-Tannoudji, M., Vandormael-Pournin, S., Drezen, J., Mercier, P., Babinet, C., and Morello, D. (2000) lacZ sequences prevent regulated expression of housekeeping genes.Mech. Dev.90, 29–39.
Whitelaw, E. and Martin, D. I. (2001) Retrotransposons as epigenetic mediators of phenotypic variation in mammals.Nat. Genet.27, 361–365.
Kwaks, T. H., Sewalt, R. G., van Blokland, R., et al. (2005) Targeting of a histone acetyltransferase domain to a promoter enhances protein expression levels in mammalian cells.J. Biotechnol.115, 35–46.
Houdebine, L. M. and Attal, J. (1999) Internal ribosome entry sites (IRESs): reality and use.Transgenic Res.8, 157–177.
Mattick, J. S. and Makunin, I. V. (2005) Small regulatory RNAs in mammals.Hum. Mol. Genet.14 (Special no. R), 121–132.
Fire, A., Xu, S., Montgomery, M. K., Kostas, S. A., Driver, S. E., and Mello, C. C. (1998) Potent and specific genetic interference by double-stranded RNA inCaenorhabditis elegans. Nature391, 806–811.
Novina, C. D. and Sharp, P. A. (2004) The RNAi revolution.Nature430, 161–164.
Unwalla, H. J., Li, M. J., Kim, J. D., Li, et al. (2004) Negative feedback inhibition of HIV-1 by TAT-inducible expression of siRNA.Nat. Biotechnol.22, 1573–1578.
Shinagawa, T. and Ishii, S. (2003) Generation of Ski-knockdown mice by expressing a long double-strand RNA from an RNA polymerase II promoter.Genes Dev.17, 1340–1345.
Xia, H., Mao, Q., Paulson, H. L., and Davidson, B. L. (2002) siRNA-mediated gene silencingin vitro andin vivo. Nat. Biotechnol.20, 1006–1010.
Gupta, S., Schoer, R. A., Egan, J. E., Hannon, G. J., and Mittal, V. (2004) Inducible, reversible, and stable RNA interference in mammalian cells.Proc. Natl. Acad. Sci. USA101, 1927–1932.
Sen, G., Wehrman, T. S., Myers, J. W., and Blau, H. M. (2004) Restriction enzyme-generated siRNA (REGS) vectors and libraries.Nat. Genet.36, 183–189.
Shirane, D., Sugao, K., Namiki, S., Tanabe, M., Iino, M., and Hirose, K. (2004) Enzymatic production of RNAi libraries from cDNAs.Nat. Genet.36, 190–196.
Hohjoh, H. (2004) Enhancement of RNAi activity by improved siRNA duplexes.FEBS Lett.557, 193–198.
Mittal, V. (2004) Improving the efficiency of RNA interference in mammals.Nat. Rev. Genet.5, 355–365.
Reynolds, A., Leake, D., Boese, Q., Scaringe, S., Marshall, W. S., and Khvorova, A. (2004) Rational siRNA design for RNA interference.Nat. Biotechnol.22, 326–330.
Ui-Tei, K., Naito, Y., Takahashi, F., et al. (2004) Guidelines for the selection of highly effective siRNA sequences for mammalian and chick RNA interference.Nucleic Acids Res.32, 936–948.
Yoshinari, K., Miyagishi, M., and Taira, K. (2004) Effects on RNAi of the tight structure, sequence and position of the targeted region.Nucleic Acids Res.32, 691–699.
Williams, B. R. (2005) Dicing with siRNA.Nat. Biotechnol.23, 181–182.
Chalk, A. M., Wahlestedt, C., and Sonnhammer, E. L. (2004) Improved and automated prediction of effective siRNA.Biochem. Biophys. Res. Commun319, 264–274.
Schwarz, D. S., Hutvagner, G., Du, T., Xu, Z., Aronin, N., and Zamore, P. D. (2003) Asymmetry in the assembly of the RNAi enzyme complex.Cell115, 199–208.
Khvorova, A., Reynolds, A., and Jayasena, S. D. (2003) Functional siRNAs and miRNAs exhibit strand bias.Cell115, 209–216.
Kim, D. H., Behlke, M. A., Rose, S. D., Chang, M. S., Choi, S., and Rossi, J. J. (2005) Synthetic dsRNA Dicer substrates enhance RNAi potency and efficacy.Nat. Biotechnol.23, 222–226.
Siolas, D., Lerner, C., Burchard, J., et al. (2005) Synthetic sh RNA as potent RNAi triggers.Nat. Biotechnol.23, 227–231.
Luo, K. Q. and Chang, D. C. (2004) The gene-silencing efficiency of siRNA is strongly dependent on the local structure of mRNA at the targeted region.Biochem. Biophys. Res. Commun.318, 303–310.
Snove, O., Jr., and Holen, T. (2004) Many commonly used siRNAs risk off-target activity.Biochem. Biophys. Res. Commun.319, 256–263.
Judge, A. D., Sood, V., Shaw, J. R., Fang, D., McClintock, K., and Maclachlan, I. (2005) Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA.Nat. Biotechnol.23, 457–462.
Yelin, R., Dahary, D., Sorek, R., et al. (2003) Widespread occurrence of antisense transcription in the human genome.Nat. Biotechnol.21, 379–386.
Carmichael, G. G. (2003) Antisense starts making more sense.Nat. Biotechnol.21, 371–372.
Ying, S. Y. and Lin, S. L. (2004) Intron-derived microRNAs—fine tuning of gene functions.Gene342, 25–28.
Ying, S. Y. and Lin, S. L. (2005) Intronic microRNAs.Biochem. Biophys. Res. Commun326, 515–520.
Kawasaki, H. and Taira, K. (2004) Induction of DNA methylation and gene silencing by short interfering RNAs in human cells.Nature431, 211–217.
Morris, K. V., Chan, S. W., Jacobsen, S. E., and Looney, D. J. (2004) Small interfering RNA-induced transcriptional gene silencing in human cells.Science305, 1289–1292.
Zeng, Y., Yi, R., and Cullen, B. R. (2003) MicroRNAs and small interfering RNAs can inhibit mRNA expression by similar mechanisms.Proc. Natl. Acad. Sci. USA100, 9779–9784.
He, L. and Hannon, G. J. (2004) MicroRNAs: small RNAs with a big role in gene regulation.Nat. Rev. Genet.5, 522–531.
Xie, X., Lu, J., Kulbokas, E. J., et al. (2005) Systematic discovery of regulatory motifs in human promoters and 3′ UTRs by comparison of several mammals.Nature434, 338–345.
Lee, Y., Kim, M., Han, J., et al. (2004) MicroRNA genes are transcribed by RNA polymerase II.EMBO J.23, 4051–4060.
Zeng, Y. and Cullen, B. R. (2003) Sequence requirements for micro RNA processing and function in human cells.RNA9, 112–123.
Ecclestin, A. and Eggleston, A. K. (2004) RNA interference.Nature431, 337–378.
Clayton, J. (2004) RNA interference: the silent treatment.Nature431, 599–605.
Müller, M. (2000) Increasing disease resistance in transgenic domestic, inMolecular Farming (Toutant, J. P. and Balazs, E., eds.), INRA Editions, Paris, pp. 87–98.
Jones, S. D. and Marasco, W. A. (1997) Intracellular antibodies (intrabodies): potential applications in transgenic animal research and engineered resistance to pathogens, inTransgenic Animal Generation and Use (Houdebine, L. M., ed.), Harwood Academic Publishers, Amsterdam, pp. 501–506.
Chang, P. Y., Benecke, H., Le Marchand-Brustel, Y., Lawitts, J., and Moller, D. E. (1994) Expression of a dominant-negative mutant human insulin receptor in the muscle of transgenic mice.J. Biol. Chem.269, 16,034–16,040.
Ono, E., Amagai, K., Taharaguchi, S., et al. (2004) Transgenic mice expressing a soluble form of porcine nectin-1/herpesvirus entry mediator C as a model for pseudorabies-resistant livestock.Proc. Natl. Acad. Sci. USA101, 16,150–16,155.
Chen, Y. T., Levasseur, R., Vaishnav, S., Karsenty, G., and Bradley, A. (2004) Bigenic Cre/loxP, puDeltatk conditional genetic ablation.Nucleic Acids Res.32, e161.
Saito, M., Iwawaki, T., Taya, C., et al. (2001) Diphtheria toxin receptor-mediated conditional and targeted cell ablation in transgenic mice.Nat. Biotechnol.19, 746–750.
Jiang, W., Zhou, L., Breyer, B., et al. (2001) Tetracycline-regulated gene expression mediated by a novel chimeric repressor that recruits histone deacetylases in mammalian cells.J. Biol. Chem.276, 45,168–45,174.
Weber, W. and Fussenegger, M. (2004) Approaches for trigger-inducible viral transgene regulation in gene-based tissue engineering.Curr. Opin. Biotechnol.15, 383–391.
Boutonnet, C., Boijoux, O., Bernat, S., et al. (2004) Pharmacological-based translational induction of transgene expression in mammalian cells.EMBO Rep.5, 721–727.
Cecconi, F. and Meyer, B. I. (2000) Gene trap: a way to identify novel genes and unravel their biological function.FEBS Lett.480, 63–71.
Jackson, I. J. (2001) Mouse mutagenesis on target.Nat. Genet.28, 198–200.
Medico, E., Gambarotta, G., Gentile, A., Comoglio, P. M., and Soriano, P. (2001) A gene trap vector system for identifying transcriptionally responsive genes.Nat. Biotechnol.19, 579–582.
Mitchell, K. J., Pinson, K. I., Kelly, O. G., et al. (2001) Functional analysis of secreted and transmembrane proteins critical to mouse development.Nat. Genet.28, 241–249.
Goodwin, N. C., Ishida, Y., Hartford, S., et al. (2001) DelBank: a mouse ES-cell resource for generating deletions.Nat. Genet.28, 310–311.
Houdebine, L. M. (2002) Antibody manufacture in transgenic animals and comparisons with other systems.Curr. Opin. Biotechnol.13, 625–629.
Houdebine, L. M. and Weill, B. (1999) The impact of transgenesis and cloning on cell and organ xenotransplantation to humans, inFocus on Biotechnology (Van Brockhoven, A., Shapiro, F., and Anne, J., eds.), Kluwer Academic Publishers, pp. 351–361.
Lai, L., Kolber-Simonds, D., Park, K. W., et al. (2002) Production of alpha-1, 3-galactosyltransferase knockout pigs by nuclear transfer cloning.Science295, 1089–1092.
Dai, Y., Vaught, T. D., Boone, J., et al. (2002) Targeted disruption of the alpha1, 3-galactosyltransferase gene in cloned pigs.Nat. Biotechnol.20, 251–255.
Switzer, W. M., Michler, R. E., Shanmugam, V., et al. (2001) Lack of cross-species transmission of porcine endogenous retrovirus infection to nonhuman primate recipients of porcine cells, tissues, or organs.Transplantation71, 959–965.
Oldmixon, B. A., Wood, J. C., Ericsson, T. A., et al. (2002) Porcine endogenous retrovirus transmission characteristics of an inbred herd of miniature swine.J. Virol.76, 3045–3048.
Chan, F., Bradley, A., Wensel, T. G., and Wilson, J. H. (2004) Knock-in human rhodopsin-GFP fusions as mouse models for human disease and targets for gene therapy.Proc. Natl. Acad. Sci. USA101, 9109–9114.
Boulanger, L., Mallet, S., Chense, P., et al. (2002) Advantages and limits of using the ubiquitous expressed EF1alpha promoter for transgenesisin vivo andin vitro in rabbit.Transgenic Res.11, 88.
al Gubarg, K. and Houdebine, L. M. In vivo imaging of green fluorescent protein-expressing cells in transgenic animals using fibered confocal fluorescience microscopy.Eur. J. Cell Biol., in press.
Devgan, V., Rao, M. R., and Seshagiri, P. B. (2004) Impact of embryonic expression of enhanced green fluorescent protein on early mouse development.Biochem. Biophys. Res. Commun.313, 1030–1036.
Pailhoux, E., Vigier, B., Chaffaux, S., et al. (2001) A 11.7-kb deletion triggers intersexuality and polledness in goats.Nat. Genet.29, 453–458.
Vaiman, D. (2003) Sexy transgenes: the impact of gene transfer and gene inactivation technologies on the understanding of mammalian sex determination.Transgenic Res.12, 255–269.
Shillingford, J. M. and Henneighausen, L. (2001) Experimental mouse genetics-answering fundamental questions about mamary gland biology.Trends Endocrinol Metab12, 402–408.
Kong, J. and Xu, Z. (2000) Overexpression of neurofilament subunit NF-L and NF-H extends survival of a mouse model for amyotrophic lateral sclerosis.Neurosci Lett.281, 72–74.
Moll, J., Barzaghi, P., Lin, S., et al. (2001) An agrin minigene rescues dystrophic symptoms in a mouse model for congenital muscular dystrophy.Nature413, 302–307.
Esler, W. P. and Wolfe, M. S. (2001) A portrait of Alzheimer secretases—new features and familiar faces.Science293, 1449–1454.
Lewis, J., Dickson, D. W., Lin, W. L., et al. (2001) Enhanced neurofibrillary degeneration in transgenic mice expressing mutant tau and APP.Science293, 1487–1491.
Chapman, P. F., Falinska, A. M., Knevett, S. G., and Ramsay, M. F. (2001) Genes, models and Alzheimer’s disease.Trends Genet.17, 254–261.
Moore, R. C. and Melton, D. W. (1997) Transgenic analysis of prion diseases. Mol. Hum. Reprod.3, 529–544.
Prusiner, S. B., Scott, M. R., DeArmond, S. J., and Cohen, F. E. (1998) Prion protein biology.Cell93, 337–348.
Scott, M. R., Will, R., Ironside, J., et al. (1999) Compelling transgenetic evidence for transmission of bovine spongiform encephalopathy prions to humans.Proc. Natl. Acad. Sci. USA96, 15,137–15,142.
Raeber, A. J., Race, R. E., Brandner, S., et al. (1997) Astrocyte-specific expression of hamster prion protein (PrP) renders PrP knockout mice susceptible to hamster scrapie.EMBO J.16, 6057–6065.
Scott, M. R., Safar, J., Telling, G., et al. (1997) Identification of a prion protein epitope modulating transmission of bovine spongiform encephalopathy prions to transgenic mice.Proc. Natl. Acad. Sci. USA94, 14,279–14,284.
Manolakou, K., Beaton, J., McConnell, I., et al. (2001) Genetic and environmental factors modify bovine spongiform encephalopathy incubation period in mice.Proc. Natl. Acad. Sci. USA98, 7402–7407.
Vilette, D., Andreoletti, O., Archer, F., et al. (2001) Ex vivo propagation of infectious sheep scrapie agent in heterologous epithelial cells expressing ovine prion protein.Proc. Natl. Acad. Sci. USA98, 4055–4059.
Betarbet, R., Sherer, T. B., and Greenamyre, J. T. (2002) Animal models of Parkinson’s disease.Bioessays24, 308–318.
Rubinsztein, D. C. (2002) Lessons from animal models of Huntington’s disease.Trends Genet.18, 202–209.
Ranger, A. M., Malynn, B. A., and Korsmeyer, S. J. (2001) Mouse models of cell death.Nat. Genet.28, 113–118.
De Boer, J., Andressoo, J. O., De Wit, J., et al. (2002) Premature aging in mice deficient in DNA repair and transcription.Science296, 1276–1279.
Miller, M. W. and Rubin, E. M. (1997) Transgenic animals in atherosclerosis research, inTransgenic Animal and Generation and Use (L. M. Houdebine, ed.), Harwood Academic Publishers, Amsterdam, pp. 445–448.
Fan, J. and Watanabe, T. (2003) Transgenic rabbits as therapeutic protein bioreactors and human disease models.Pharmacol. Ther.99, 261–282.
Siegel, P. M., Hardy, W. R., and Muller, W. J. (2000) Mammary gland neoplasia: insights from transgenic mouse models.Bioessays22, 554–563.
Bartek, J. and Lukas, J. (2001) Are all cancer genes equal?Nature411, 1001–1002.
Yu, Q., Geng, Y., and Sicinski, P. (2001) Specific protection against breast cancers by cyclin D1 ablation.Nature411, 1017–1021.
Schwertfeger, K. L., Richert, M. M., and Anderson, S. M. (2001) Mammary gland involution is delayed by activated Akt in transgenic mice.Mol. Endocrinol.15, 867–881.
Berns, A. (2001) Cancer. Improved mouse models.Nature410, 1043–1044.
Johnson, L., Mercer, K., Greenbaum, D., et al. (2001) Somatic activation of the K-ras oncogene causes early onset lung cancer in mice.Nature410, 1111–1116.
Lecuit, M., Vandormael-Pournin, S., Lefort, J., et al. (2001) A transgenic model for listeriosis: role of internalin in crossing the intestinal barrier.Science292, 1722–1725.
Oldstone, M. B., Lewicki, H., Thomas, D., et al. (1999) Measles virus infection in a transgenic model: virus-induced immunosuppression and central nervous system disease.Cell98, 629–640.
Ren, R. B., Costantini, F., Gorgacz, E. J., Lee, J. J., and Racaniello, V. R. (1990) Transgenic mice expressing a human poliovirus receptor: a new model for poliomyelitis.Cell63, 353–362.
Fausto, N. (2001) A mouse model for hepatitis C virus infection?Nat. Med.7, 890–891.
Dunn, C. S., Mehtali, M., Houdebine, L. M., Gut, J. P., Kirn, A., and Aubertin, A. M. (1995) Human immunodeficiency virus type 1 infection of human CD4-transgenic rabbits.J. Gene. Virol.76, 1327–1336.
Cohen, J. (2001) Building a small-animal model for AIDS, block by block.Science293, 1034–1036.
Reid, W., Sadowska, M., Denaro, F., et al. (2001) An HIV-1 transgenic rat that develops HIV-related pathology and immunologic dysfunction.Proc. Natl. Acad. Sci. USA98, 9271–9276.
Carvallo, G., Canard, G., and Tucker, D. (1997) Standardization of transgenic lines: from founder to an established animal model, inTransgenic Animal Generation and Use (Houdebine, L. M., ed.), Harwood Academic Publishers, Amsterdam, pp. 403–410.
Auerbach, A. B., Norinsky, R., Ho, W., et al. (2003) Strain-dependent differences in the efficiency of transgenic mouse production.Transgenic Res.12, 59–69.
Abbott, A. (2004) Geneticists prepare for deluge of mutant mice.Nature432, 541.
Valenzuela, D. M., Murphy, A. J., Frendewey, D., et al. (2003) High-throughput engineering of the mouse genome coupled with high-resolution expression analysis.Nat. Biotechnol.21, 652–659.
Kuroiwa, Y., Kasinathan, P., Matsushita, H., et al. (2004) Sequential targeting of the genes encoding immunoglobulin-micro and prion protein in cattle.Nat. Genet.36, 671–672.
Zhou, Q., Renard, J. P., Le Friec, G., et al. (2003) Generation of fertile cloned rats by regulating oocyte activation.Science302, 1179.
Chesne, P., Adenot, P. G., Viglietta, C., Baratte, M., Boulanger, L., and Renard, J. P. (2002) Cloned rabbits produced by nuclear transfer from adult somatic cells.Nat. Biotechnol.20, 366–369.
Kwaks, T. H., Barnett, P., Hemrika, W., et al. (2003) Identification of anti-repressor elements that confer high and stable protein production in mammalian cells.Nat. Biotechnol.21, 553–558.
Szathmary, E., Jordan, F., and Pal, C. (2001) Molecular biology and evolution. Can genes explain biological complexity?Science292, 1315–1316.
Liggett, S. B. (2004) Genetically modified mouse models for pharmacogenomic research.Nat. Rev. Genet.5, 657–663.
Lee, D. and Threadgill, D. W. (2004) Investigating gene function using mouse models.Curr. Opin. Genet. Dev.14, 246–252.
Kues, W. A. and Niemann, H. (2004) The contribution of farm animals to human health.Trends Biotechnol.22, 286–294.
Moore, A. (2001) Of mice and Mendel. The predicted rise in the use of knock-out and transgenic mice should cause us to reflect on our justification for the use of animals in research.EMBO Rep.2, 554–558.
Acknowledgment
I thank Annie Paglino for her help in the preparation of the manuscript.
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Biologie du Developpement et Reproduction, Institut National de la Recherche Agronomique, Paris, France
Louis-Marie Houdebine
- Louis-Marie Houdebine
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Department of Immunology, Institute for Cancer Research, The Norwegian Radium Hospital, University of Oslo, Oslo, Norway
Mouldy Sioud
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Houdebine, LM. (2007). Transgenic Animal Models in Biomedical Research. In: Sioud, M. (eds) Target Discovery and Validation Reviews and Protocols. Methods in Molecular Biology™, vol 360. Humana Press. https://doi.org/10.1385/1-59745-165-7:163
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