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Genetic Detoxification of Bacterial Toxins

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Part of the book series:Methods in Molecular Medicine™ ((MIMM,volume 4))

Abstract

Several pathogens, such asCorynebacterium diphtheriae, Clostridium tetani, Bordetella pertussis, Vibrio cholerae, enterotoxigenicEscherichia co1i (1), and even some emerging pathogens, such asHelicobacter pylori (2), produce potent toxins that are responsible for the pathology caused by the bacterium. In most cases the disease, and often even the infection, can be prevented by a vaccine that induces immunity against the toxin. In order to be used in vaccines, the dangerous toxins need to be depleted of their toxic activity in an effective and irreversible manner. The most effective way to inactivate toxins for inclusion in vaccines was developed by Ramon in 1924 by using formaldehyde treatment at 37°C to detoxify diphtheria toxin (3). This method was then used to inactivate other toxins and also viral and bacterial suspensions. Even today, widely used vaccines, such as diphtheria, tetanus, inactivated polio, and whole cell pertussis, and even some of the newly developed acellular pertussis vaccines are produced using formaldehyde or other chemical treatments to inactivate the toxin and/or kill the microorganisms that are present in the vaccine (4,5).

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Author information

Authors and Affiliations

  1. Immunobiological Research Institute, Siena, Italy

    Mariagrazia Pizza, Maria Rita Fontana, Vincenzo Scarlato & Rino Rappuoli

Authors
  1. Mariagrazia Pizza

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  2. Maria Rita Fontana

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  3. Vincenzo Scarlato

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  4. Rino Rappuoli

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Editor information

Editors and Affiliations

  1. Centre for Applied Microbiology and Research, Porton Down, UK

    Andrew Robinson , Graham H. Farrar  & Christopher N. Wiblin ,  & 

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© 1996 Humana Press Inc, Totowa, NJ

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Pizza, M., Fontana, M.R., Scarlato, V., Rappuoli, R. (1996). Genetic Detoxification of Bacterial Toxins. In: Robinson, A., Farrar, G.H., Wiblin, C.N. (eds) Vaccine Protocols. Methods in Molecular Medicine™, vol 4. Humana Press. https://doi.org/10.1385/0-89603-334-1:91

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