Article navigation

October 1994

Journal of Cell Science Cover Image for Volume 107, Issue 10

RESEARCH ARTICLE|01 October 1994

The fission yeastcdc19⁺ gene encodes a member of the MCM family of replication proteins

Susan L. Forsburg*

Molecular Biology and Virology Laboratory, The Salk Institute for Biological Studies, PO Box 85800, San Diego, CA 92186, USA

ICRF Cell Cycle Group, Biochemistry Department

Oxford University, South Parks Road

Oxford, UK

*Author for correspondence at address

Search for other works by this author on:

ICRF Cell Cycle Group, Biochemistry Department

Oxford University, South Parks Road

Oxford, UK

Search for other works by this author on:

This site

PubMed

Google Scholar

Author and article information

Susan L. Forsburg*

Molecular Biology and Virology Laboratory, The Salk Institute for Biological Studies, PO Box 85800, San Diego, CA 92186, USA

ICRF Cell Cycle Group, Biochemistry Department

Oxford University, South Parks Road

Oxford, UK

Paul Nurse

ICRF Cell Cycle Group, Biochemistry Department

Oxford University, South Parks Road

Oxford, UK

*Author for correspondence at address

†

Present address: Imperial Cancer Research Fund, 44 Lincoln’s Inn Fields, London WC2A 3PX, UK

Received:14 Apr 1994

Online ISSN: 1477-9137

Print ISSN: 0021-9533

1994

J Cell Sci (1994) 107 (10): 2779–2788.

https://doi.org/10.1242/jcs.107.10.2779

Article history

Received:

14 Apr 1994

ABSTRACT

We have cloned and characterized the fission yeastcdc19⁺ gene. We demonstrate that it encodes a structural homologue of the budding yeast MCM2 protein. In fission yeast, thecdc19⁺ gene is constitutively expressed, and essential for viability. Deletion delays progression through S phase, and cells arrest in the first cycle with an apparent 2C DNA content, with their checkpoint control intact. The temperature-sensitivecdc19-P1 mutation is synthetically lethal withcdc21-M68. In addition, we show by classical and molecular genetics thatcdc19⁺ is allelic to thenda1⁺ locus. We conclude that cdc19p plays a potentially conserved role in S phase.

Keywords:

cdc19,DNA replication,MCM2,fission yeast,cell cycle

1994

You do not currently have access to this content.

Sign in

Don't already have an account?Register

Client Account

You could not be signed in. Please check your email address / username and password and try again.

Email address / Username?

Password

Could not validate captcha. Please try again.

Reset password

Sign in via your institution

Pay-Per-View Access

$30.00

Buy This Article

Email alerts

Article activity alert

Accepted manuscripts alert

Table of contents alert

Latest published articles alert

Close Modal

886Views

62Crossref

View Metrics

Cited by

Google Scholar

Crossref (62)

Call for papers - Cilia and Flagella: from Basic Biology to Disease

We are welcoming submissions for our upcoming special issue:Cilia and Flagella: from Basic Biology to Disease. This issue will be coordinated by two Guest Editors: Pleasantine Mill (University of Edinburgh) and Lotte Pedersen (University of Copenhagen). Extended submission deadline: 31 March 2025.

History of our journals

As our publisher, The Company of Biologists, turns 100 years old, read aboutJournal of Cell Science’s journey and explore the history of each of our sister journals:Development,Journal of Experimental Biology,Disease Models & Mechanisms andBiology Open.

Introducing our new Associate Editors

In this Editorial, JCS Editor-in-Chief Michael Waywelcomes five new Associate Editors to the JCS team. These Associate Editors will expand our support for the wider cell biology community and handle articles in immune cell biology, proteostasis, imaging and image analysis, plant cell biology, and stem cell biology and modelling.

Diversity of microtubule arrays in animal cells at a glance

In this Cell Science at a Glance article,Emma van Grinsven and Anna Akhmanova provide an overview of the diverse microtubule arrays present in differentiated animal cells and discuss how these arrays form and function.

JCS-FocalPlane Training Grants

Supporting microcopy training for early-career researchers in cell biology - apply for a grant up to £1,000

Early-career researchers - working in an area covered by JCS - who would like to attend a microscopy training course, pleaseapply. Deadline dates for 2025 applications: 7 March 2025 (decision by week commencing 21 April 2025) and 6 June 2025 (decision by week commencing 28 July 2025).