We have sublocalized the human proto-oncogenec-myb by applying two different techniques: in situ hybridization of metaphase spreads and chromosome spot hybridization of flow-sorted chromosomes. For this we used a teratocarcinoma cell line carrying specific chromosome translocations involving the two chromosomes 6 and one chromosome 11. The distribution of thec-myb gene copies on the different translocation chromosomes revealed thatc-myb is located in the region 6q21→q23. Because of the close proximity of thec-myb locus to the chromosomal breakpoints in the teratocarcinoma, we investigated whetherc-myb was implicated in the development of this tumor. No rearrangement, deletion, or amplification of the gene was detected in the teratocarcinoma cells. Furthermore, the level ofc-myb expression was comparable to that of other cell lines of nonhematopoietic origin. These results suggest thatc-myb was not affected by the translocation and played no significant role in the development of this teratocarcinoma.

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1986

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