In Vivo Time-Lapse Imaging of Neuronal Development inXenopus
Adapted fromImaging in Developmental Biology (ed. Sharpe and Wong). CSHL Press, Cold Spring Harbor, NY, USA, 2011.Abstract
In vivo fluorescence imaging of cells in the developing nervous system is greatly facilitated in specimens in which cells are brightly but sparsely labeled. In this article, we describe a number of techniques that can be used for delivering fluorophore to neurons in the albinoXenopus laevis tadpole. Fluorescent dye or DNA that encodes a fluorescent protein can be delivered to single cells by electroporation. Alternatively, multiple cells can be labeled with fluorescent dye introduced by local iontophoresis or with plasmid DNA introduced by bulk electroporation. Technical considerations and analysis methods for time-lapse imaging in living tissue are also discussed.
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