YPD media
MATERIALS
Reagents
Bacto agar (Becton, Dickinson and Company) (if preparing agar plates)
Bacto peptone (Becton, Dickinson and Company)
Glucose (40%, w/v)
Sterilize by filtration or autoclaving.
Yeast extract (Fisher Scientific)
Equipment
Autoclave
Flasks, autoclavable
Petri dishes, plastic, 9-cm diameter (if preparing agar plates)
Stir bar
Stir plate, magnetic
METHOD
1.To an autoclavable flask, add:
Reagent Amount to add (per 1 L final volume) Liquid Agar plates Bacto agar -- 24 g Bacto peptone 20 g 20 g Yeast extract 10 g 10 g Water 950 mL 950 mL 2.Autoclave the mixture.
3.Prepare as appropriate:
For liquid media
i.Add 50 mL of sterile 40% (w/v) glucose. Mix.
ii.Allow to cool before use.
For agar plates
iii.While stirring the autoclaved mixture on a magnetic stir plate, add 50 mL of sterile 40% glucose per liter of media.
iv.While still warm, pour media into 9-cm diameter plastic Petri dishes.
v.Allow agar mixture to cool and solidify.
