How to CultivateEctocarpus
- Susana M. Coelho1,2,4,
- Delphine Scornet1,2,
- Sylvie Rousvoal1,2,
- Nick T. Peters1,2,
- Laurence Dartevelle1,2,
- Akira F. Peters2,3 and
- J. Mark Cock1,2
- 1UPMC Université Paris 06, The Marine Plants and Biomolecules Laboratory, UMR 7139, Station Biologique de Roscoff, BP74, 29682 Roscoff Cedex, France
- 2CNRS, UMR 7139, Laboratoire International Associé Dispersal and Adaptation in Marine Species, Station Biologique de Roscoff, BP74, 29682 Roscoff Cedex, France
- 3Bezhin Rosko, 29250 Santec, France
Abstract
This article describes the standard procedure for growingEctocarpus in the laboratory. The culture is started with partheno-sporophyte (or sporophyte) filaments because this is the stage that is usually maintained in strain collections. The standard medium is Provasoli-enriched natural seawater (PES), butEctocarpus can also be grown in artificial seawater, which allows more precise control over the culture conditions. The algae can be cultivated either in plastic Petri dishes or in 10-L bottles with bubbling, if large amounts of biomass are required. Standard growth conditions are 13°C with a 12h/12h d/night cycle and 20 µmol photons m−2 s−1 irradiance using daylight-type fluorescent tubes. All manipulations ofEctocarpus cultures should be performed in a clean environment (if possible, under a laminar flow hood). Forceps should be dipped in ethanol and allowed to dry under the hood.
Footnotes
↵4 Correspondence:coelho{at}sb-roscoff.fr
- © 2012 Cold Spring Harbor Laboratory Press
