Enterobacter strains 090008T and 090028T were recovered from the blood of two different patients at West China Hospital, Chengdu, PR China in 2017. Phylogenetic analysis based on the 16S rRNA gene and multi-locus sequence analysis of therpoB,gyrB,infB andatpD housekeeping genes revealed that the two strains were distinct from any previously known species of the genusEnterobacter. Both average nucleotide identity (ANI) andin silico DNA–DNA hybridization (isDDH) values between strains 090008T, 090028T and type strains of all knownEnterobacter species were lower than the cut-off (≥95–96 % for ANI and ≥70 % for isDDH) to define a bacterial species. The major cellular fatty acids of strains 090008T and 090028T are C_16 : 0, C_17 : 0cyclo and C_18 : 1ω7c, which are similar to otherEnterobacter species, and the genomic DNA G+C content was 55.73 and 55.68 mol%, respectively. Strain 090008T can be differentiated from otherEnterobacter species by its ability to ferment sucrose, melibiose andd-arabitol, but with a negative methyl α-d-mannopyranoside reaction. Strain 090028T can ferment potassium gluconate, but is negative forl-fucose, mdecarboxylase, deaminase aelibiose andd-sorbitol, which distinguishes it from all otherEnterobacter species. Genotypic and phenotypic characteristics indicate that strains 090008T and 090028T represent two novel species of the genusEnterobacter, for which the namesEnterobacter huaxiensissp. nov. andEnterobacter chuandaensis sp. nov. are proposed, respectively. The type strain ofEnterobacter huaxiensissp. nov. is 090008T (=GDMCC1.1426T=CCTCC AB 2018174T=CNCTC 7648T) and the type strain ofEnterobacter chuandaensissp. nov. is 090028T (=GDMCC1.1427T=CCTCC AB 2018173T=CNCTC 7649T).