Movatterモバイル変換

[0]ホーム
URL:

Skip to content
1887
  • search icon
  • account icon
  • shopping cart icon

    • Other

    Free

    Reclassification of strains MAFF 303099T and R7A intoMesorhizobiumjaponicum sp. nov.Free

    Abstract

    In this work we revise the taxonomic status of theLotus-nodulating strains MAFF 303099T and R7A isolated in Japan and New Zealand, respectively. Their 16S rRNA gene sequences are identical and show 98.0, 99.7, 99.8 and 99.9 % similarity values with respect toMesorhizobium loti NZP 2213T,M. jarvisii ATCC 33669T,M. huakuii USDA 4779T (=CCBAU 2609T) andM. erdmanii USDA 3471T, respectively. The analysis ofrecA andglnII gene sequeces showed thatM. jarvisii ATCC 33669T andM. huakuii USDA 4779T (=CCBAU 2609T) are the most closely related strains to MAFF 303099T and R7A, with similarity values suggesting that these two strains belong to a different species for which MAFF 303099T is selected as the type strain. The DNA–DNA relatedness values between strain MAFF 303099T and its closest phylogenetic relatives ranged from 53 to 60 % in average. Strains MAFF 303099T and R7A presented slight differences in the proportions of C18 : 1ω7c 11-methyl and C19 : 0 cyclo ω8c fatty acids with respect toM. jarvisii ATCC 33669T andM. huakuii USDA 4779T, and also in several phenotypic characteristics. Therefore, we propose the reclassification of these two strains into a novel species namedMesorhizobium japonicum sp. nov., with the type strain being MAFF 303099T (=LMG 29417T=CECT 9101T).

    • Received:
    • Accepted:
    • Published Online:
    Keyword(s):Lotus,MesorhizobiumandTaxonomy
    © 2016 IUMS
    Loading

    Article metrics loading...

    /content/journal/ijsem/10.1099/ijsem.0.001448
    2016-12-01
    2025-04-02
    Download as PowerPoint
    Loading full text...

    Full text loading...

    /deliver/fulltext/ijsem/66/12/4936.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001448&mimeType=html&fmt=ahah

    References

    1. BeringerJ. E.1974; R factor transfer in Rhizobium leguminosarum.J Gen Microbiol 84:188–198 [View Article][PubMed]
      [Google Scholar]
    2. EzakiT.,HashimotoY.,YabuuchiE.1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains.Int J Syst Bacteriol 39:224–229 [View Article]
      [Google Scholar]
    3. KanekoT.,NakamuraY.,SatoS.,AsamizuE.,KatoT.,SasamotoS.,WatanabeA.,IdesawaK.,IshikawaA. et al.2000; Complete genome structure of the nitrogen-fixing symbiotic bacteriumMesorhizobium loti.DNA Res 7:331–338 [View Article][PubMed]
      [Google Scholar]
    4. KellyS.,SullivanJ.,RonsonC.,TianR.,BräuL.,MunkC.,GoodwinL.,HanC.,WoykeT. et al.2014; Genome sequence of theLotus spp. microsymbiontMesorhizobium loti strain R7A.Stand Genomic Sci 9:6 [View Article][PubMed]
      [Google Scholar]
    5. KimuraM.1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences.J Mol Evol 16:111–120 [View Article][PubMed]
      [Google Scholar]
    6. Martínez-HidalgoP.,Ramírez-BahenaM. H.,Flores-FélixJ. D.,RivasR.,IgualJ. M.,MateosP. F.,Martínez-MolinaE.,León-BarriosM.,PeixÁ.,VelázquezE.2015; Revision of the taxonomic status of type strains ofMesorhizobium loti and reclassification of strain USDA 3471T asMesorhizobium erdmanii sp. nov. and ATCC 33669TMesorhizobium jarvisii sp. nov.Int J Syst Evol Microbiol 65:1703–1708 [View Article][PubMed]
      [Google Scholar]
    7. Ormeño-OrrilloE.,Servín-GarcidueñasL. E.,RogelM. A.,GonzálezV.,PeraltaH.,MoraJ.,Martínez-RomeroJ.,Martínez-RomeroE.2015; Taxonomy of rhizobia and agrobacteria from theRhizobiaceae family in light of genomics.Syst Appl Microbiol 38:287–291 [View Article][PubMed]
      [Google Scholar]
    8. PeixA.,Ramírez-BahenaM. H.,VelázquezE.,BedmarE. J.2015; Bacterial associations with legumes.Crit Rev Plant Sci 34:17–42 [View Article]
      [Google Scholar]
    9. Ramírez-BahenaM. H.,HernándezM.,PeixA.,VelázquezE.,León-BarriosM.2012; Mesorhizobial strains nodulatingAnagyris latifolia andLotus berthelotii in Tamadaya ravine (Tenerife, Canary Islands) are two symbiovars of the same species,Mesorhizobium tamadayense sp. nov.Syst Appl Microbiol 35:334–341 [View Article][PubMed]
      [Google Scholar]
    10. RogersJ. S.,SwoffordD. L.1998; A fast method for approximating maximum likelihoods of phylogenetic trees from nucleotide sequences.Syst Biol 47:77–89[PubMed][CrossRef]
      [Google Scholar]
    11. SaekiK.,KouchiH.2000; TheLotus symbiont,Mesorhizobium loti: molecular genetic techniques and application.J Plant Res 113:457–465 [View Article]
      [Google Scholar]
    12. SaitouN.,NeiM.1987; A neighbour-joining method: a new method for reconstructing phylogenetics trees.Mol Biol Evol 4:406–425[PubMed]
      [Google Scholar]
    13. SasserM.1990Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids MIDI Technical Note 101 Newark, DE: MIDI Inc;
      [Google Scholar]
    14. SullivanJ. T.,PatrickH. N.,LowtherW. L.,ScottD. B.,RonsonC. W.1995; Nodulating strains ofRhizobium loti arise through chromosomal symbiotic gene transfer in the environment.Proc Natl Acad Sci U S A 92:8985–8989 [View Article][PubMed]
      [Google Scholar]
    15. TamuraK.,PetersonD.,PetersonN.,StecherG.,NeiM.,KumarS.2011;mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods.Mol Biol Evol 28:2731–2739 [View Article][PubMed]
      [Google Scholar]
    16. ThompsonJ. D.,GibsonT. J.,PlewniakF.,JeanmouginF.,HigginsD. G.1997; Theclustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools.Nucleic Acid Res 25:4876–4882 [View Article][PubMed]
      [Google Scholar]
    17. TurnerS. L.,ZhangX. X.,LiF. D.,YoungJ. P.2002; What does a bacterial genome sequence represent? mis-assignment of MAFF 303099 to the genospeciesMesorhizobium loti.Microbiology 148:3330–3331 [View Article][PubMed]
      [Google Scholar]
    18. VargheseN. J.,MukherjeeS.,IvanovaN.,KonstantinidisK. T.,MavrommatisK.,KyrpidesN. C.,PatiA.2015; Microbial species delineation using whole genome sequences.Nucleic Acids Res 43:6761–6771 [View Article][PubMed]
      [Google Scholar]
    19. VincentJ. M.1970; Manual for the practical study of the RootNodule bacteria. InIBP Handbookvol. 15 Oxford: Blackwell;
      [Google Scholar]
    20. WayneL. G.,BrennerD. J.,ColwellR. R.,GrimontP. A. D.,KandlerO.,KrichevskyM. I.,MooreL. H.,MooreW. E. C.,MurrayR. G. E. et al.1987; Report of thead hoc committee on reconciliation of approaches to bacterial systematics.Int J Syst Evol Microbiol 37:463–464 [View Article]
      [Google Scholar]
    21. WillemsA.,Doignon-BourcierF.,GorisJ.,CoopmanR.,de LajudieP.,De VosP.,GillisM.2001; DNA–DNA hybridization study ofBradyrhizobium strains.Int J Syst Evol Microbiol 51:1315–1322 [View Article][PubMed]
      [Google Scholar]
    /content/journal/ijsem/10.1099/ijsem.0.001448
    Loading
    Reclassification of strains MAFF 303099T and R7A into Mesorhizobiumjaponicum sp. nov.
    Int J Syst Evol Microbiol66, 4936 (2016);https://doi.org/10.1099/ijsem.0.001448
    /content/journal/ijsem/10.1099/ijsem.0.001448
    /content/journal/ijsem/10.1099/ijsem.0.001448
    Loading

    Data & Media loading...

    Supplements

    Supplementary File 1

    PDF

    Most read this month

    Article
    content/journal/ijsem
    Journal
    5
    3
    false
    en
    Loading

    Most citedMost Cited RSS feed

    We Recommend

    Access key

    • Subscribed Subscribed content
    • Open Access Open Access content
    • Free Trial Free Trial content
    • Frees Free content

    Discover publishing discounts and more

    Microbiology Society members enjoy discounted Open Access publishing in all of our titles, as well as access to a range of grants, professional development opportunities and event discounts.

    Join today.

    Fee Free Open Access Publishing via Publish and Read

    Are you eligible forfree Open Access publishing via a Publish and Read agreement inInternational Journal of Systematic and Evolutionary Microbiology, the official publication for novel microbial taxa? Check if your institution is signed uphere

    This is a required field
    Please enter a valid email address
    Approval was a Success
    Invalid data
    An Error Occurred
    Approval was partially successful, following selected items could not be processed due to error
    Microbiology Society:
    http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001448
    10.1099/ijsem.0.001448
    SEARCH_EXPAND_ITEM
    [8]ページ先頭
    ©2009-2025 Movatter.jp