Campylobacter avium sp. nov., a hippurate-positive species isolated from poultry

Mirko Rossi^1,4,Lies Debruyne²,Renato Giulio Zanoni¹,Gerardo Manfreda³,Joana Revez¹ andPeter Vandamme²
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¹1Department of Veterinary Public Health and Animal Pathology, Alma Mater Studiorum, University of Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia, Bologna, Italy²2Department of Biochemistry, Physiology and Microbiology, Faculty of Sciences, Ghent University, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium³3Department of Food Science, Alma Mater Studiorum, University of Bologna, Via del Florio 2, 40064 Ozzano Emilia, Bologna, Italy
CorrespondenceMirko Rossi [email protected]
4 †Present address: Department of Food and Environmental Hygiene, University of Helsinki, PO Box 66, Agnes Sjöbergin katu 2, FI-0001 Helsinki, Finland.
Published:01 September 2009https://doi.org/10.1099/ijs.0.007419-0

Abstract

Three strains of an unusual hippurate-positiveCampylobacter species were isolated at 37 °C from caecal contents of broiler chickens and a turkey. All strains were initially identified asCampylobacter by means of genus-specific PCR, but none was further identified using specific PCRs for known thermophilic species. Phylogenetic analyses based on 16S rRNA,rpoB andgroEL gene sequences revealed that these strains formed a robust clade distinct from otherCampylobacter species. Amplified fragment length polymorphism analysis and whole-cell protein electrophoresis were subsequently carried out and confirmed the divergence between the avian strains and other taxa. These data indicate that the unidentifiedCampylobacter strains belong to a novel taxon which could be distinguished from other campylobacters through its phenotypic and genotypic characteristics. The nameCampylobacter avium sp. nov., is proposed for the novel species, with the type strain 86/06T (=LMG 24591T =CCUG 56292T).

Published Online:01/09/2009

Keyword(s):AFLP, amplified fragment length polymorphism

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Campylobacter avium sp. nov., a hippurate-positive species isolated from poultry

Int J Syst Evol Microbiol59, 2364 (2009);https://doi.org/10.1099/ijs.0.007419-0

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International Journal of Systematic and EvolutionaryMicrobiology vol.59 , part 9, pp. 2364 - 2369
Supplementary Figs S1 and S2. Unrooted trees, based onrpoB (Fig. S1) andgroEL (Fig. S2) gene sequences, showing the phylogeneticrelationships of the three strains ofC. avium sp. nov.
Supplementary Fig. S3. Dendrogram of the three strainsofC. avium sp. nov. based on UPGMA cluster analysis ofone-dimensional SDS-PAGE cell protein profiles.
[PDF file of SupplementaryFigs S1-S3](102 KB)

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