Movatterモバイル変換

[0]ホーム
URL:

Skip to content
1887
  • search icon
  • account icon
  • shopping cart icon

    • Review Article

    Free

    Taxonomic relationships of the [Pasteurella]haemolytica complex as evaluated by DNA-DNA hybridizations and 16S rRNA sequencing with proposal ofMannheimia haemolytica gen. nov., comb. nov.,Mannheimia granulomatis comb. nov.,Mannheimia glucosida sp. nov.,Mannheimia ruminalis sp. nov. andMannheimia varigena sp. nov.Free

    Abstract

    The present paper presents the conclusions of a polyphasic investigation of the taxonomy of the trehalose-negative [Pasteurella]haemolytica complex. Clusters previously identified by ribotyping and multilocus enzyme electrophoresis (MEE) have been evaluated by 16S rRNA sequencing and DNA–DNA hybridizations. Results obtained by the different techniques were highly related and indicated that the [P.]haemolytica complex contains distinct genetic and phenotypic groups. At least seven species were outlined, five of which were named. We refrained in formal naming of more groups until additional strains are characterized. Five 16S rRNA clusters were identified corresponding to distinct lineages previously outlined by MEE. Within 16S rRNA cluster I two distinct genotypic groups have been outlined in addition to [P.]haemolytica sensu stricto (biogroup 1). Each of the clusters II, III, IV and V represent at least one new species. The investigations underline that [P.]haemolytica sensu stricto only contains strains that donot fermentl-arabinose even though they are referred to as ‘biotype A’ of [P.]haemolytica. The five 16S rRNA clusters identified had a common root relative to the other species within the familyPasteurellaceae, and the overall sequence similarity among these five clusters was higher than what is observed within the existing genera of the family. The allocation of the trehalose-negative [P.]haemolytica complex to a new genus seems to be indicated. Based on the polyphasic investigation performed a new genusMannheimia is proposed for the trehalose-negative [P.]haemolytica complex. At the present stage two previously named species are transferred to this new genus and three new species are described. [P.]haemolytica is reclassified asMannheimia haemolytica comb. nov., whereasPasteurella granulomatis, Bisgaard taxon 20 and [P.]haemolytica biovar 3J are reclassified and combined in the speciesMannheimia granulomatis comb. nov.Mannheimia glucosida sp. nov. corresponds to [P.]haemolytica biogroups 3A-3H and theβ-glucosidase andmeso-inositol-positive strains of [P.]haemolytica biogroup 9. All typable strains withinM.glucosida belong to serotype 11.Mannheimia ruminalis sp. nov. consists of strains previously classified as Bisgaard taxon 18 and [P.]haemolytica biogroup 8D. Finally,Mannheimia varigena sp. nov. includes [P.]haemolytica biogroup 6 as well as Bisgaard taxon 15 and Bisgaard taxon 36. The type strains are NCTC 9380T (M.haemolytica), ATCC 49244T (M.granulomatis), CCUG 38457T = P925T (M.glucosida). CCUG 38470T = HPA92T (M.ruminalis) and CCUG 38462T = 177T (M.varigena).

    • Published Online:
    Keyword(s):16s rRNA sequencing,DNA–DNA hybridization,Mannheimia gen. nov.,Pasteurella haemolyticaandpolyphasic taxonomy
    © 1999, International Union of Microbiological Societies
    Loading

    Article metrics loading...

    /content/journal/ijsem/10.1099/00207713-49-1-67
    1999-01-01
    2026-02-18

    Metrics

    Download as PowerPoint
    Loading full text...

    Full text loading...

    /deliver/fulltext/ijsem/49/1/ijs-49-1-67.html?itemId=/content/journal/ijsem/10.1099/00207713-49-1-67&mimeType=html&fmt=ahah

    References

    1. AngenØ.,AalbaekB.,FalsenE.,OlsenJ. E.,BisgaardM.1997a; Phenotypical relationship among strains classified with the ruminant [Pasteurella]haemolytica-complex using quantitative evaluation of phenotypic data.Zentbl Bakteriol 285:459–479
      [Google Scholar]
    2. AngenØ.,OlsenJ. E.,BisgaardM.1997b; Further studies on the relationships among strains classified as taxon 15, taxon 18, taxon 20, [Pasteurella]granulomatis or the [Pasteurella]haemolytica-complex in ruminants using quantitative evaluation of phenotypic data.Zentbl Bakteriol 286:317–332
      [Google Scholar]
    3. AngenØ.,CaugantD. A.,OlsenJ. E.,BisgaardM.1997c; Genotypic relationships among strains classified within the [Pasteurella]haemolytica-complex as indicated by ribotyping and multilocus enzyme electrophoresis.Zentbl Bakteriol 286:333–354
      [Google Scholar]
    4. AngenØ.,QuirieM.,DonachieW.,BisgaardM.1999; Investigations on the species specificity ofMannheimia (Pasteurella)haemolytica serotyping.Vet Microbiol (in press)
      [Google Scholar]
    5. BibersteinE. L.,FrancisC. K.1968; Nucleic acid homologies between the A and T types ofPasteurella haemolytica.J Med Microbiol 1:105–108
      [Google Scholar]
    6. BibersteinE. L.,GillsM. G.1962; The relationship of the antigenic types of the A and T types ofPasteurella haemolytica.J Comp Pathol 72:316–320
      [Google Scholar]
    7. BibersteinE. L.,ThompsonD. A.1966; Epidemiological studies onPasteurella haemolytica in sheep.J Comp Pathol 76:83–94
      [Google Scholar]
    8. BibersteinE. L.,GillsM.,KnightH.1960; Serological types ofPasteurella haemolytica.Cornell Vet 50:283–300
      [Google Scholar]
    9. BinghamD. P.,MooreR.,RichardsA. B.1990; Comparison of DNA:DNA homology and enzymatic activity betweenPasteurella haemolytica and related species.Am J Vet Res 51:1161–1166
      [Google Scholar]
    10. BisgaardM.1984; Comparative investigations ofPasteurella haemolytica sensu stricto and so-calledPhaemolytica isolated from different pathological lesions in pigs.Acta Pathol Microbiol Immunol Scand Sect B 92:201–207
      [Google Scholar]
    11. BisgaardM.1995; Taxonomy of the familyPasteurellaceae Pohl 1981.In Haemophilus, Actinobacillus, and Pasteurella1–7DonachieW.,LainsonF. A.,HodgsonJ. C.Proceedings from the Third International Conference onHaemophilusActinobacillusand PasteurellaJuly31August41993 Edinburgh, UK. London: Plenum;
      [Google Scholar]
    12. BisgaardM.,MuttersR.1986; Reinvestigations of selected bovine and ovine strains previously classified asPasteurella haemolytica and description of some new taxa within thePasteurella haemolytica-complex.Acta Pathol Microbiol Immunol Scand Sect B 94:185–193
      [Google Scholar]
    13. BisgaardM.,PhillipsJ. E.,MannheimW.1986; Characterization and identification of bovine and ovinePasteurellaceae isolated from the oral cavity and rumen of apparently normal cattle and sheep.Acta Pathol Microbiol Immunol Scand Sect B 94:9–17
      [Google Scholar]
    14. BrosiusJ.,PalmerM. L.,KennedyP. J.,NollerH. F.1978; Complete nucleotide sequence of a 16S ribosomal RNA gene fromEscherichia coli.Proc Natl Acad SciUSA 75:4801–4905
      [Google Scholar]
    15. BurrowsL. L.,Olah-WinfieldE.,LoR. Y. C.1993; Molecular analyses of the leucotoxin determinants fromPasteurella haemolytica serotypes 1 to 16.Infect Immun 61:5001–5007
      [Google Scholar]
    16. DaviesR. L.,PasterB. J.,DewhirstF. E.1996; Phylogenetic relationships and diversity within thePasteurella haemolytica complex based on 16S rRNA sequence comparison and outer membrane protein and lipopolysaccharide analysis.Int J Syst Bacteriol 46:736–744
      [Google Scholar]
    17. DaviesR. L.,ArkinsawS.,SelanderR. K.1997; Evolutionary genetics ofPasteurella haemolytica isolates recovered from cattle and sheep.Infect Immun 65:3585–3593
      [Google Scholar]
    18. De LeyJ.,CattoirH.,ReynaertsA.1970; The quantitative measurement of DNA hybridization from renaturation rates.Eur J Biochem 12:133–142
      [Google Scholar]
    19. De LeyJ.,MannheimW.,MuttersR.7 other authors1990; Inter- and intrafamilial similarities of the rRNA cistrons of thePasteurellaceae.Int J Syst Bacteriol 40:126–137
      [Google Scholar]
    20. DevrieseL. A.,BisgaardM.,HommezJ.,UyttebroeckE.,DucatelleR.,HaesebrouckF.1991; Taxon 20 (Fam.Pasteurellaceae) infections in European brown hares (Lepus europaeus).J Wildl Dis 27:685–687
      [Google Scholar]
    21. DewhirstF. E.,PasterB. J.,OlsenI.,FraserG. J.1992; Phylogeny of 54 representative strains of species in the familyPasteurellaceae as determined by comparison of 16S rRNA sequences.J Bacteriol 174:2002–2013
      [Google Scholar]
    22. DewhirstF. E.,PasterB. J.,OlsenI.,FraserG. J.1993; Phylogeny of thePasteurellaceae determined by comparison of 16S ribosomal ribonucleic acid sequences.Zentbl Bakteriol 279:35–44
      [Google Scholar]
    23. DonachieW.,FraserJ.,QuirieM.,GilmourN. J. L.1984; Studies on strains ofPasteurella haemolytica not typable by the indirect haemagglutination test.Res Vet Sci 37:188–193
      [Google Scholar]
    24. FodorL.,VargaJ.,HajtosI.,DonachieW.,GilmourN. J.1987; New serotype ofPasteurella haemolytica isolated in Hungary.Vet Rec 121:155
      [Google Scholar]
    25. FoxG. E.,WisotzkeyJ. D.,JurtshukP. J.1992; How close is close: 16S rRNA sequence identity may not be sufficient to guarantee species identity.Int J Syst Bacteriol 42:166–170
      [Google Scholar]
    26. FrankG. H.1989; Pasteurellosis of cattle.In Pasteurella and Pasteureliosis197–222AdlamC.,RutterJ. M.London: Academic Press;
      [Google Scholar]
    27. FraserJ.,LairdS.,GilmourN. J. L.1982; A new serovar (biovar T) ofPasteurella haemolytica.Res Vet Sci 32:127–128
      [Google Scholar]
    28. FrederiksenW.1973;Pasteurella taxonomy and nomenclature.Contrib Microbiol Immun 2:170–176
      [Google Scholar]
    29. GillisM.,De LeyJ.,De CleeneM.1970; The determination of molecular weight of bacterial genome DNA from renaturation rates.Eur J Biochem 12:143–153
      [Google Scholar]
    30. GilmourN. J. L.,GilmourJ. S.1989; Pasteurellosis of sheep.In Pasteurella and Pasteurellosis223–262AdlamC.,RutterJ. M.London: Academic Press;
      [Google Scholar]
    31. JonesF.1921; A study ofBacillus bovisepticus.J Exp Med 34:561–577
      [Google Scholar]
    32. JukesT. H.,CantorC. R.1969; Evolution of protein molecules.In Mammalian Protein Metabolism21–132MunroH. N.New York: Academic Press;
      [Google Scholar]
    33. KirbyK. S.,Fox-CarterE.,GuestM.1967; Isolation of deoxyribonucleic acid and ribosomal ribonucleic acid from bacteria.Biochem J 104:258–262
      [Google Scholar]
    34. LaneD.1991; 16S/23S rRNA sequencing.In Nucleic Acid Techniques in Bacterial Systematics115–147StackebrandtE.,GoodfellowM.New York: Wiley;
      [Google Scholar]
    35. LeeC. W.,ShewenP. E.,CladmanW. M.,ConlonJ. A. R.,MellorsA.,LoR. Y. C.1994; Sialoglycoprotease ofPasteurella haemolytica A1 : detection of antisialoglycoprotease antibodies in sera of calves.Can J Vet Res 58:93–98
      [Google Scholar]
    36. McLaughlinB. G.,GreerS. C.,ChengappaM. M.,SinghS.,MadduxR. L.,KadelW. L.,McLaughlinP. S.1991; Association of aPasteurella haemolytica-like organism with enteritis in swine.J Vet Diagn Invest 3:324–327
      [Google Scholar]
    37. MannheimW.1983; Taxonomy of the familyPasteurellaceae Pohl 1981 as revealed by DNA:DNA hybridization.INSERM 114:211–226
      [Google Scholar]
    38. MarmurJ.1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms.J Mol Biol 3:208–218
      [Google Scholar]
    39. MarmurJ.,DotyP.1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature.J Mol Biol 5:109–118
      [Google Scholar]
    40. MrazO.1969; Vergleichende Studie der ArtenActinobacillus lignieresii undPasteurella haemolytica II.Pasteurella haemolyticaNewsom and Cross, 1932.Zentbl Bakteriol Parasitendk Infektkrankh Hyg I Abt 209:336–349
      [Google Scholar]
    41. MurrayJ. E.,DaviesR. C.,LainsonF. A.,WilsonC. F.,DonachieW.1992; An ti genie analysis of iron-regulated proteins inPasteurella haemolytica A and T biotypes by immunoblotting reveals biotype-specific epitopes.J Gen Microbiol 138:283–288
      [Google Scholar]
    42. MurrayR. G. E.,BrennerD. J.,De VosP.,ColwellR. R.,GoodfellowM.,GrimontP. A. D.,PfennigN.,StackebrandtE.,ZavarzinG. A.1990; International Committee on Systematic Bacteriology. Report of the ad hoc committee on approaches to taxonomy within theProteobacteria.Int J Syst Bacteriol 40:213–215
      [Google Scholar]
    43. MuttersR.,BisgaardM.,PohlS.1986; Taxonomic relationship of selected biogroups ofPasteurella haemolytica as revealed by DNA : DNA hybridizations.Acta Pat hoi Microbiol Immunol Scand Sect B 94:195–202
      [Google Scholar]
    44. MuttersR.,IhmP.,PohlS.,FrederiksenW.,MannheimW.1985; Reclassification of the genusPasteurella Trevisan 1887 on the basis of deoxyribonucleic acid homology, with proposals for the new speciesPasteurella dagmatisPasteurella canisPasteurella stomatisPasteurella anatis andPasteurella langaa.Int J Syst Bacteriol 35:309–322
      [Google Scholar]
    45. MuttersR.,MannheimW.,BisgaardM.1989; Taxonomy of the group.In Pasteurella and Pasteurelloses3–34AdlamC.,RutterJ. M.London: Academic Press;
      [Google Scholar]
    46. MuttersR.,PiechullaK.,HinzK.-H.,MannheimW.1985;Pasteurella avium (Hinz and Kunjara 1977) comb. nov. andPasteurella volantium sp. nov.Int J Syst Bacteriol 35:5–9
      [Google Scholar]
    47. NewsomI. E.,CrossF.1932; Some bipolar organisms found in pneumonia in sheep.J Am Vet Med Assoc 80:711–719
      [Google Scholar]
    48. OlsenG. J.,MatsudaH.,HagstromR.,OverbeekR.1994; fastDNAml : a tool for construction of phylogenetic trees of DNA sequences using maximum likelihood.Comput Appl Biosci 10:41–48
      [Google Scholar]
    49. OsawaR.,RaineyF.,FujisawaT.,LangE.,BusseH. J.,WalshT. P.,StackebrandtE.1995;Lonepinella koalarum gen. nov., sp. nov., a new tannin-protein complex degrading bacterium.Syst Appl Microbiol 18:368–373
      [Google Scholar]
    50. OsawaR.,RaineyF.,FujisawaT.,LangE.,BusseH. J.,WalshT. P.,StackebrandtE.1996;Lonepinella koalarum gen. nov., sp. nov.In Validation of the Publication of New Names and New Combinations Previously Effectively Published Outside the USB List no. 56.Int J Syst Bacteriol 46:362–363
      [Google Scholar]
    51. PasterB. J.,DewhirstF. E.1988; Phylogeny of campylobacters, wolinellas,Bacteroides gracilis andBacteroides ureolyticus by 16S ribosomal ribonucleic acid sequencing.Int J Syst Bacteriol 38:56–62
      [Google Scholar]
    52. PegramR. G.,RoederP. L.,ScottJ. M.1979; Two new serotypes ofPasteurella haemolytica from sheep in Ethiopia.Trop Anim Health Prod 11:29–30
      [Google Scholar]
    53. PhillipsJ. E.1961; The commensal role ofActinobacillus lignieresii.J Pathol Bacteriol 82:205–208
      [Google Scholar]
    54. PhillipsJ. E.1964; Commensal actinobacilli from the bovine tongue.J Pathol Bacteriol 87:442–444
      [Google Scholar]
    55. PhillipsJ. E.1966;Actinobacillus lignieresii: a study of the organism and its association with its hosts.DVM & S thesis University of Edinburgh;
      [Google Scholar]
    56. PohlS.1979;Reklassifiezierung der Gattung Actinobacillus Brumpt 1910,Haemophilus Winslow et al 1917and Pasteurella Trevisan 1887anhand phänotypischer und molekular Dateninbesondere der DNS-Verwandtschaften bei DNADNA-Hybridisierung in vitro und Vorschlag einer neuen FamiliePasteurellaceae.Thesis Fachbereich Biologie der Philipps-Universität Marburg/Lahn;
      [Google Scholar]
    57. QuirieM.,DonachieW.,GilmourN. J.1986; Serotypes ofPasteurella haemolytica from cattle.Vet Rec 119:93–94
      [Google Scholar]
    58. RibeiroG. A.,CarterG. R.,FrederiksenW.,Riet-CorreaF.1989;Pasteurella haemolytica-like bacterium from a progressive granuloma of cattle in Brazil.J Clin Microbiol 27:1401–1402
      [Google Scholar]
    59. RibeiroG. A.,CarterG. R.,FrederiksenW.,Riet-CorreaF.1990;Pasteurella granulomatis sp. nov. InValidation of the Publication of New Names and New Combinations Previously Effectively Published Outside the USB. List no. 32Int J Syst Bacteriol 40:105–106
      [Google Scholar]
    60. Riet-CorreaF.,MéndezM. C.,SchildA. L.,RibeiroG. A.,AlmeidaS. M.1992; Bovine focal progressive fibro-granulomatous panniculitis (Lechiguana) associated withPasteurella granulomatis.Vet Pathol 29:93–103
      [Google Scholar]
    61. RohlfF. J.1993;ntsys-pc.Numerical taxonomy and multi-variate analysis system Version 1.80 New York: Applied Biostatistics;
      [Google Scholar]
    62. SangerF.,NicklenS.,CoulsonA. R.1977; DNA sequencing with chain-termina ting inhibitors.Proc Natl Acad SciUSA 74:5463–5467
      [Google Scholar]
    63. SmithG. R.1959; Isolation of two types ofPasteurella haemolytica from sheep.Nature 183:1132–1133
      [Google Scholar]
    64. SmithG. R.1961; The characteristics of two types ofPasteurella haemolytica associated with different pathological conditions in sheep.J Pathol Bacteriol 81:431–440
      [Google Scholar]
    65. SneathP. H. A.,SokalR. R.1973Numerical Taxonomy San Francisco: Freeman;
      [Google Scholar]
    66. SneathP. H.,StevensM.1990;Actinobacillus rossii sp. nov.,Actinobacillus seminis sp. nov., nom. rev.,Pasteurella bettii sp. nov.,Pasteurella lymphangitidis sp. nov.,Pasteurella mairi sp. nov., andPasteurella trehalosi sp. nov.Int J Syst Bacteriol 40:148–153
      [Google Scholar]
    67. StackebrandtE.,GoebelB. M.1994; Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology.Int J Syst Bacteriol 44:846–849
      [Google Scholar]
    68. WayneL. G.,BrennerD. J.,ColwellR. R.9 other authors1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics.Int J Syst Bacteriol 37:463–464
      [Google Scholar]
    69. YounanM.,WallmannJ.1989; A new serovar ofPasteurella haemolytica from sheep in Syria.Trop Anim Health Prod 21:87
      [Google Scholar]
    /content/journal/ijsem/10.1099/00207713-49-1-67
    Loading
    Taxonomic relationships of the [Pasteurella] haemolytica complex as evaluated by DNA-DNA hybridizations and 16S rRNA sequencing with proposal of Mannheimia haemolytica gen. nov., comb. nov., Mannheimia granulomatis comb. nov., Mannheimia glucosida sp. nov., Mannheimia ruminalis sp. nov. and Mannheimia varigena sp. nov.
    Int J Syst Evol Microbiol49, 67 (1999);https://doi.org/10.1099/00207713-49-1-67
    /content/journal/ijsem/10.1099/00207713-49-1-67
    /content/journal/ijsem/10.1099/00207713-49-1-67
    Loading

    Data & Media loading...

    Most read this month

    Article
    content/journal/ijsem
    Journal
    5
    3
    false
    en
    Loading

    Most citedMost Cited RSS feed

    We Recommend

    Access key

    • Subscribed Subscribed content
    • Open Access Open Access content
    • Free Trial Free Trial content
    • Frees Free content

    Microbiology Horizons: the new interdisciplinary journal

    Green Mcirobiology Horizons logo with the words Discover our new journal

    Find out more about our newest title,Microbiology Horizons, bringing together insights on new technologies and solutions in microbiology to address global challenges.

    Fee Free Open Access Publishing via Publish and Read

    Are you eligible forfree Open Access publishing via a Publish and Read agreement inInternational Journal of Systematic and Evolutionary Microbiology, the official publication for novel microbial taxa? Check if your institution is signed uphere

    This is a required field
    Please enter a valid email address
    Approval was a Success
    Invalid data
    An error occurred
    Approval was partially successful, following selected items could not be processed due to error
    Microbiology Society:
    http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-49-1-67
    10.1099/00207713-49-1-67
    SEARCH_EXPAND_ITEM
    [8]ページ先頭
    ©2009-2026 Movatter.jp