ECM-based microchannel for culturingin vitro vascular tissues with simultaneous perfusion and stretch†
* Corresponding authors
a School of Integrated Design Engineering, Graduate School of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-Ku, Yokohama, Japan
E-mail:onoe@mech.keio.ac.jp
b Pillar of Engineering Product Development, Singapore University of Technology and Design, 8 Somapah Road, Singapore 487372, Singapore
c Digital Manufacturing and Design (DManD) Centre, Singapore University of Technology and Design, Singapore
d Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-Ku, Tokyo, Japan
e Department of Mechanical Engineering, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-Ku, Yokohama, Japan
Abstract
We present an extracellular matrix (ECM)-based stretchable microfluidic system for culturingin vitro three-dimensional (3D) vascular tissues, which mimicsin vivo blood vessels. Human umbilical vein endothelial cells (HUVECs) can be cultured under perfusion and stretch simultaneously with real-time imaging by our proposed system. Our ECM (transglutaminase (TG) cross-linked gelatin)-based microchannel was fabricated by dissolving water-soluble sacrificial polyvinyl alcohol (PVA) molds printed with a 3D printer. Flows in the microchannel were analyzed under perfusion and stretch. We demonstrated simultaneous perfusion and stretch of TG gelatin-based microchannels culturing HUVECs. We suggest that our TG gelatin-based stretchable microfluidic system proves to be a useful tool for understanding the mechanisms of vascular tissue formation and mechanotransduction.
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Article information
- Article type
- Paper
- Submitted
- 12 Mar 2020
- Accepted
- 11 Apr 2020
- First published
- 20 Apr 2020
Permissions
ECM-based microchannel for culturingin vitro vascular tissues with simultaneous perfusion and stretch
A. Shimizu, W. H. Goh, S. Itai, M. Hashimoto, S. Miura and H. Onoe,Lab Chip, 2020, 20, 1917DOI: 10.1039/D0LC00254B
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