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Nature Methods
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Unexpected failure rates for modular assembly of engineered zinc fingers

Nature Methodsvolume 5, pages374–375 (2008)Cite this article

AnErratum to this article was published on 01 June 2008

This article has beenupdated

NOTE: In the version of this correspondence initially published, the two previously published datasets analyzed were labeled with incorrect references in Figure 1b. Reference 2 should be associated with the second column (80 sites) and Reference 3 should be associated with the third column (96 sites). The weblink for the Zinc Finger Consortium website was incorrect and should be http://www.zincfingers.org/. The errors have been corrected in the HTML and PDF versions of the article.

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Figure 1: Large-scale evaluation of the modular assembly method for engineering zinc-finger arrays.

Change history

  • 29 May 2008

    In the version of this correspondence initially published, the two previously published datasets analyzed were labeled with incorrect references in Figure 1b. Reference 2 should be associated with the second column (80 sites) and Reference 3 should be associated with the third column (96 sites). The weblink for the Zinc Finger Consortium website was incorrect and should be http://www.zincfingers.org/. The errors have been corrected in the HTML and PDF versions of the article.

References

  1. Porteus, M.H. & Carroll, D.Nat. Biotechnol.23, 967–973 (2005).

    Article CAS  Google Scholar 

  2. Segal, D.J. et al.Biochemistry42, 2137–2148 (2003).

    Article CAS  Google Scholar 

  3. Bae, K.H. et al.Nat. Biotechnol.21, 275–280 (2003).

    Article CAS  Google Scholar 

  4. Mandell, J.G. & Barbas, C.F.Nucleic Acids Res.34, W516–W523 (2006).

    Article CAS  Google Scholar 

  5. Liu, Q., Xia, Z., Zhong, X. & Case, C.C.J. Biol. Chem.277, 3850–3856 (2002).

    Article CAS  Google Scholar 

  6. Wright, D.A. et al.Nat. Protoc.1, 1637–1652 (2006).

    Article  Google Scholar 

Download references

Acknowledgements

We thank D. Dobbs and P. Zaback for helpful discussions and E. Unger-Wallace (Iowa State University) for contributions to zinc-finger array construction. J.K.J. is supported by the US National Institutes of Health (R21 RR024189), the Cystic Fibrosis Foundation (MCCRAY07G0) and the Massachusetts General Hospital Department of Pathology; D.F.V. is supported by a US National Science Foundation grant DBI 0501678; T.C. is supported by the German Research Foundation (SPP1230 CA311/2); C.L.R. is supported by a Ford Foundation Pre-Doctoral Diversity Fellowship and a National Science Foundation Graduate Research Fellowship; T.I.C. is supported by a fellowship from the Swiss Foundation for Grants in Biology and Medicine.

Author information

Author notes

  1. Cherie L Ramirez and Jonathan E Foley: These authors contributed equally to this work.

Authors and Affiliations

  1. Molecular Pathology Unit, Center for Cancer Research, and Center for Computational and Integrative Biology, 149 13th Street, Room 7132, Massachusetts General Hospital, Charlestown, 02129, Massachusetts, USA

    Cherie L Ramirez, Jonathan E Foley & J Keith Joung

  2. Division of Medical Sciences, Biological and Biomedical Sciences Program, Harvard Medical School, Boston, 02115, Massachusetts, USA

    Cherie L Ramirez & J Keith Joung

  3. Department of Genetics, Development and Cell Biology, Iowa State University, 1035A Roy J. Carver Co-Lab, Ames, 50011, Iowa, USA

    David A Wright, Ronnie J Winfrey, Jeffry D Sander, Fengli Fu, Jeffrey A Townsend & Daniel F Voytas

  4. Charité Medical School, Institute of Virology, Hindenburgdamm 27, Berlin, D-12203, Germany

    Felix Müller-Lerch, Shamim H Rahman, Tatjana I Cornu & Toni Cathomen

  5. Interdepartmental Graduate Program in Bioinformatics and Computational Biology, 2114 Molecular Biology Building, Iowa State University, Ames, 50011, Iowa, USA

    Jeffry D Sander & Fengli Fu

  6. Department of Pathology, Harvard Medical School, Boston, 02115, Massachusetts, USA

    J Keith Joung

Authors
  1. Cherie L Ramirez

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  2. Jonathan E Foley

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  3. David A Wright

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  4. Felix Müller-Lerch

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  5. Shamim H Rahman

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  6. Tatjana I Cornu

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  7. Ronnie J Winfrey

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  8. Jeffry D Sander

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  9. Fengli Fu

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  10. Jeffrey A Townsend

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  11. Toni Cathomen

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  12. Daniel F Voytas

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  13. J Keith Joung

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Corresponding author

Correspondence toJ Keith Joung.

Supplementary information

Supplementary Text and Figures

Supplementary Figures 1–4, Supplementary Tables 1–2, Supplementary Discussion, Supplementary Methods (PDF 319 kb)

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Ramirez, C., Foley, J., Wright, D.et al. Unexpected failure rates for modular assembly of engineered zinc fingers.Nat Methods5, 374–375 (2008). https://doi.org/10.1038/nmeth0508-374

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