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Definition of a consensus binding site for p53
Nature Geneticsvolume 1, pages45–49 (1992)Cite this article
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Abstract
Recent experiments have suggested that p53 action may be mediated through its inter action with DNA. We have now identified 18 human genomic clones that bind to p53in vitro. Precise mapping of the binding sequences within these clones revealed a consensus binding site with a striking internal symmetry, consisting of two copies of the 10 base pair motif 5′-PuPuPuC(A/T)(T/A)GPyPyPy-3′ separated by 0-13 base pairs. One copy of the motif was insufficient for binding, and subtle alterations of the motif, even when present in multiple copies, resulted in loss of affinity for p53. Mutants of p53, representing each of the four “hot spots” frequently altered in human cancers, failed to bind to the consensus dimer. These results define the DNA sequence elements with which p53 interactsin vitro and which may be important for p53 actionin vivo.
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Authors and Affiliations
Oncology Center, Johns Hopkins University School of Medicine, 424 N Bond Street, Baltimore, Maryland, 21231, USA
Wafik S. El-Deiry, Scott E. Kern, Jennifer A. Pietenpol, Kenneth W. Kinzler & Bert Vogelstein
Department of Pathology, Johns Hopkins University School of Medicine, 424 N Bond Street, Baltimore, Maryland, 21231, USA
Scott E. Kern
- Wafik S. El-Deiry
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- Scott E. Kern
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- Jennifer A. Pietenpol
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- Kenneth W. Kinzler
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- Bert Vogelstein
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El-Deiry, W., Kern, S., Pietenpol, J.et al. Definition of a consensus binding site for p53.Nat Genet1, 45–49 (1992). https://doi.org/10.1038/ng0492-45
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