Movatterモバイル変換

[0]ホーム
URL:

Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Advertisement

Nature
  • Letter
  • Published:

PNA hybridizes to complementary oligonucleotides obeying the Watson–Crick hydrogen-bonding rules

Naturevolume 365, pages566–568 (1993)Cite this article

Abstract

DNA ANALOGUES are currently being intensely investigated owing to their potential as gene-targeted drugs1–3. Furthermore, their properties and interaction with DNA and RNA could provide a better understanding of the structural features of natural DNA that determine its unique chemical, biological and genetic properties3,4. We recently designed a DNA analogue, PNA, in which the backbone is structurally homomorphous with the deoxyribose backbone and consists ofN-(2-aminoethyl)glycine units to which the nucleobases are attached5–9. We showed that PNA oligomers containing solely thymine and cytosine can hybridize to complementary oligonucleotides, presumably by forming Watson–Crick–Hoogsteen (PNA)2–DNA triplexes, which are much more stable than the corresponding DNA–DNA duplexes5–7, and bind to double-stranded DNA by strand displacement5,8. We report here that PNA containing all four natural nucleobases hybridizes to complementary oligonucleotides obeying the Watson–Crick base-pairing rules, and thus is a true DNA mimic in terms of base-pair recognition.

This is a preview of subscription content,access via your institution

Access options

Access through your institution

Subscription info for Japanese customers

We have a dedicated website for our Japanese customers. Please go tonatureasia.com to subscribe to this journal.

Buy this article

  • Purchase on SpringerLink
  • Instant access to full article PDF

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Hélène, C. & Toulmé, J.-J.Biochim. biophys. Acta1049, 99–125 (1990).

    Article  Google Scholar 

  2. Goodchild, J.Bioconjugate Chem.1, 165–187 (1990).

    Article CAS  Google Scholar 

  3. Uhlmann, E. & Peyman, A.Chem. Rev.90, 544–584 (1990).

    Article  Google Scholar 

  4. Eschenmoser, A. & Loewenthal, E.Chem. Soc. Rev.21, 1–16 (1992).

    Article CAS  Google Scholar 

  5. Nielsen, P. E., Egholm, M., Berg, R. H. & Buchardt, O.Science254, 1497–1500 (1991).

    Article ADS CAS  Google Scholar 

  6. Egholm, M., Buchardt, O., Nielsen, P. E. & Berg, R. H.J. Am. Chem. Soc.114, 1895–1897 (1992).

    Article CAS  Google Scholar 

  7. Egholm, M., Buchardt, O., Nielsen, P. E. & Berg, R. H.J. Am. Chem. Soc.114, 9677–9678 (1992).

    Article CAS  Google Scholar 

  8. Egholm, M.et al.J. chem. Soc. chem. Commun. 800–801 (1993).

  9. Cherny, D. Y.et al.Proc. natn. Acad. Sci. U.S.A.90, 1667–1670 (1993).

    Article ADS CAS  Google Scholar 

  10. Borer, P. N., Dengler, B., Tinoco, I. Jr. & Uhlenbeck, O. C.J. molec. Biol86, 843–853 (1974).

    Article CAS  Google Scholar 

  11. Freier, S. M., Burger, M. D., Alkema, D., Neilson, T. & Turner, D.Biochemistry22, 6198–6206 (1983).

    Article CAS  Google Scholar 

  12. Vesnaver, G. & Breslauer, K. J.Proc. natn. Acad. Sci. U.S.A.88, 3569–3573 (1991).

    Article ADS CAS  Google Scholar 

  13. Orgel, L.Nature358, 203–209 (1992).

    Article ADS CAS  Google Scholar 

  14. Cech, T.Proc. natn. Acad. Sci. U.S.A.83, 4360–4364 (1986).

    Article ADS CAS  Google Scholar 

  15. Miller, S. L.Science117, 528–529 (1953).

    Article ADS CAS  Google Scholar 

  16. Oro, J.Biochem. biophys. Res. Commun.2, 407–415 (1960).

    Article  Google Scholar 

  17. Crook, S. T.Curr. Opinion. Biotech.3, 656–661 (1992).

    Article  Google Scholar 

  18. Hanvey, J. C.et al.Science258, 1481–1485 (1992).

    Article ADS CAS  Google Scholar 

  19. Nielsen, P. E., Egholm, M., Berg, R. H. & Buchardt, O.Anti-Cancer Drug Design8, 53–63 (1993).

    CAS PubMed  Google Scholar 

  20. Freier, S. M., Albergo, D. D. & Turner, D.Biopolymers22, 1107 (1983).

    Article CAS  Google Scholar 

  21. Maniatis, T., Fritsch, E. & Sambrook, J.Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Laboratory Press, New York, 1982).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Organic Chemistry, The H. C. Ørsted Institute, Universitetsparken 5, DK-2100 Ø, Copenhagen, Denmark

    Michael Egholm, Ole Buchardt, Leif Christensen & Carsten Behrens

  2. ISIS Pharmaceuticals, 2292 Faraday Avenue, Carlsbad, California, 82008, USA

    Susan M. Freier & David A. Driver

  3. Polymer Group, Materials Department, Risø National Laboratory, DK, 4000, Roskilde, Denmark

    Rolf H. Berg

  4. Department of Physical Chemistry, Chalmers University of Technology, S-41296, Gothenburg, Sweden

    Seog K. Kim & Bengt Norden

  5. Research Center for Medical Biotechnology, Department of Biochemistry B, The Panum Institute, Blegdamsvej 3c, 2200, Copenhagen N, Denmark

    Peter E. Nielsen

Authors
  1. Michael Egholm

    You can also search for this author inPubMed Google Scholar

  2. Ole Buchardt

    You can also search for this author inPubMed Google Scholar

  3. Leif Christensen

    You can also search for this author inPubMed Google Scholar

  4. Carsten Behrens

    You can also search for this author inPubMed Google Scholar

  5. Susan M. Freier

    You can also search for this author inPubMed Google Scholar

  6. David A. Driver

    You can also search for this author inPubMed Google Scholar

  7. Rolf H. Berg

    You can also search for this author inPubMed Google Scholar

  8. Seog K. Kim

    You can also search for this author inPubMed Google Scholar

  9. Bengt Norden

    You can also search for this author inPubMed Google Scholar

  10. Peter E. Nielsen

    You can also search for this author inPubMed Google Scholar

Rights and permissions

About this article

Cite this article

Egholm, M., Buchardt, O., Christensen, L.et al. PNA hybridizes to complementary oligonucleotides obeying the Watson–Crick hydrogen-bonding rules.Nature365, 566–568 (1993). https://doi.org/10.1038/365566a0

Download citation

Access through your institution
Buy or subscribe

Advertisement

Search

Advanced search

Quick links

Nature Briefing

Sign up for theNature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox.Sign up for Nature Briefing
[8]ページ先頭
©2009-2025 Movatter.jp