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Conservation of the sequence and temporal expression oflet-7 heterochronic regulatory RNA

Naturevolume 408, pages86–89 (2000)Cite this article

Abstract

Two small RNAs regulate the timing ofCaenorhabditis elegans development1,2. Transition from the first to the second larval stage fates requires the 22-nucleotidelin-4 RNA1,3,4, and transition from late larval to adult cell fates requires the 21-nucleotidelet-7 RNA2. Thelin-4 andlet-7 RNA genes are not homologous to each other, but are each complementary to sequences in the 3′ untranslated regions of a set of protein-coding target genes that are normally negatively regulated by the RNAs1,2,5,6. Here we have detectedlet-7 RNAs of∼21 nucleotides in samples from a wide range of animal species, including vertebrate, ascidian, hemichordate, mollusc, annelid and arthropod, but not in RNAs from several cnidarian and poriferan species, Saccharomyces cerevisiae,Escherichia coli orArabidopsis. We did not detectlin-4 RNA in these species. We found thatlet-7 temporal regulation is also conserved:let-7 RNA expression is first detected at late larval stages inC. elegans andDrosophila, at 48 hours after fertilization in zebrafish, and in adult stages of annelids and molluscs. Thelet-7 regulatory RNA may control late temporal transitions during development across animal phylogeny.

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Figure 1:let-7 gene sequences.
Figure 2: Expression oflet-7 RNA in human andDrosophila.
Figure 3: Expression oflet-7 RNA is developmentally regulated in lophotrochozoans and deuterostomes.
Figure 4: Phylogenetic comparison oflet-7 RNA expression.

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Acknowledgements

We thank the following people for RNA and tissue samples: T. Heanue, R. Pearse and C. Tabin for chick; J. Gerhart and M. Kirschner forXenopus and acorn worm; S. Agarwal forXenopus; N. Stavropoulos for mouse; C. Unabia and K. del Carmen for annelid and mollusc; H. Bode for Hydra; J. Nardone and S. Ferrari forArabidopsis; P. Sudarsanam for yeast; and D. Selinger forE. coli. The phylogenetic survey in this work was inspired by the NASA Evolution and Development meetings organized by E. Davidson and C. Golden. This work was supported by an NIH grant from NIGMS to G.R. and a grant from the MGH Fund for Medical Discovery to A.E.P.

Author information

Author notes

  1. Amy E. Pasquinelli, Brenda J. Reinhart, Patrick Leahy and Eric Davidson: These authors contributed equally to this work

Authors and Affiliations

  1. Department of Molecular Biology Massachusetts General Hospital, and Department of Genetics, Harvard Medical School, Boston, 02114, Massachusetts, USA

    Amy E. Pasquinelli, Brenda J. Reinhart & Gary Ruvkun

  2. Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, 06520, Connecticut, USA

    Frank Slack & Betsy Maller

  3. Kewalo Marine Lab, Pacific Biomedical Research Center, University of Hawaii, Honolulu, 96813, Hawaii , USA

    Mark Q. Martindale

  4. Howard Hughes Medical Institute, Baylor College of Medicine, Houston, 77030, Texas, USA

    Mitzi I. Kuroda

  5. Research School of Biological Sciences, PO Box 475, Australian National University, Canberra, 2601, ACT, Australia

    David C. Hayward & Eldon E. Ball

  6. Department of Zoology and Entomology University of Queensland, Brisbane, 4072, Queensland, Australia

    Bernard Degnan

  7. Institute of Zoology, University of Basel, Basel, CH-4051, Switzerland

    Peter Müller & Jürg Spring

  8. Cardiovascular Research Center, Massachusetts General Hospital, Charlestown, Massachusetts, 02129, USA

    Ashok Srinivasan & Mark Fishman

  9. Department of Biology, Boston University, Boston, 02215, Massachusetts, USA

    John Finnerty

  10. Department of Molecular and Cell Biology, University of California, Berkeley, 94720, California , USA

    Joseph Corbo & Michael Levine

  11. Division of Biology 156-29, California Institute of Technology, Pasadena, 91125, California, USA

    Patrick Leahy & Eric Davidson

Authors
  1. Amy E. Pasquinelli
  2. Brenda J. Reinhart
  3. Frank Slack
  4. Mark Q. Martindale
  5. Mitzi I. Kuroda
  6. Betsy Maller
  7. David C. Hayward
  8. Eldon E. Ball
  9. Bernard Degnan
  10. Peter Müller
  11. Jürg Spring
  12. Ashok Srinivasan
  13. Mark Fishman
  14. John Finnerty
  15. Joseph Corbo
  16. Michael Levine
  17. Patrick Leahy
  18. Eric Davidson
  19. Gary Ruvkun

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Pasquinelli, A., Reinhart, B., Slack, F.et al. Conservation of the sequence and temporal expression oflet-7 heterochronic regulatory RNA.Nature408, 86–89 (2000). https://doi.org/10.1038/35040556

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