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The codon CUG is read as serine in an asporogenic yeastCandida cylindracea

Naturevolume 341, pages164–166 (1989)Cite this article

Abstract

DEVIATIONS from the universal genetic code have been reported for several microorganisms. Termination codons are used for cod-ing some amino acids inParamecium1,2,Mycoplasma3 orTetrahymena4,5, and inEscherichia coli, the UGA termination codon is used to code for selenocysteine6. In mitochondria, the changes of sense codons to termination codons or to codons encoding other amino acids have also been reported7,8. Here we report another example of divergence from the universal code, this time in a non-spore-forming yeastCandida cylindracea, in which the uni-versal codon for leucine, CUG, is used to code for serine. This conclusion is based on the observations that: (1) the amino-acid composition and the partial amino-acid sequences of an extracel-lular lipase from this yeast agreed with those deduced from the complementary DNA if CUG was assumed to specify serine; and (2) serine, but not leucine, was incorporated into a polypeptide in a cell-free translation system from this yeast in the presence of a synthetic CUG oligomer.

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References

  1. Caron, F. & Meyer, E.Nature314, 185–188 (1985).

    Article ADS CAS  Google Scholar 

  2. Preer, J. R. Jr, Preer, L. B., Rudman, B. M. & Barnett, A. J.Nature314, 188–190 (1985).

    Article ADS CAS  Google Scholar 

  3. Yamao, F.et al.Proc. natn. Acad Sci. U.S.A.82, 2306–2309 (1985).

    Article ADS CAS  Google Scholar 

  4. Horowitz, S. & Gorovsky, M. A.Proc. natn Acad. Sci. U.S.A.82, 2452–2455 (1985).

    Article ADS CAS  Google Scholar 

  5. Kuchino, Y., Hanyu, N., Tashiro, F. & Nishimura, S.Proc. natn. Acad. Sci. U.S.A.82, 4758–4762 (1985).

    Article ADS CAS  Google Scholar 

  6. Leinfelder, W., Zehelein, E., Mandrand-Berthelot, M.-A. & Böck, A.Nature331, 723–725 (1988).

    Article ADS CAS  Google Scholar 

  7. Bonitz, S. G.et al.Proc. natn. Acad Sci. U.S.A.77, 3167–3170 (1980).

    Article ADS CAS  Google Scholar 

  8. Anderson, S.et al.Nature290, 457–465 (1981).

    Article ADS CAS  Google Scholar 

  9. Yamada, K. & Machida, H.Nippon Nōgeikagaku kaishi36, 858–860 (1962).

    Article CAS  Google Scholar 

  10. Tomizuka, N., Ota, Y. & Yamada, K.Agric. Biol. Chem.30, 576–584 (1966).

    CAS  Google Scholar 

  11. Normanly, J., Ogden, R. C., Horvath, S. J. & Abelson, J.Nature321, 213–219 (1986).

    Article ADS CAS  Google Scholar 

  12. Inokuchi, H., Hoben, P., Yamao, F., Ozeki, H. & Söll, D.Proc. natn. Acad. Sci. U.S.A.81, 5076–5080 (1984).

    Article ADS CAS  Google Scholar 

  13. Gorini, L. inRibosomes, 791–803 (Cold Spring Harbor Laboratory, New York, 1974).

    Google Scholar 

  14. Yarus, M. & Thompson, R. inGene Function in Prokaryotes (eds Beckwith, J., Davies, J. & Gallant, J. A.) 23–63 (Cold Spring Harbor Laboratory, New York, 1983).

    Google Scholar 

  15. Osawa, S. & Jukes, T. H.Trends Genet.4, 191–198 (1988).

    Article CAS  Google Scholar 

  16. Maniatis, T., Fritsch, E. F. & Sambrook, J. inMolecular Cloning: A Laboratory Manual 196 (Cold Spring Harbor Laboratory, New York, 1982).

    Google Scholar 

  17. Young, R. A. & Davis, R. W.Proc. natn. Acad Sci. USA.80, 1194–1198 (1983).

    Article ADS CAS  Google Scholar 

  18. Young, R. A. & Davis, R. W.Science222, 778–782 (1983).

    Article ADS CAS  Google Scholar 

  19. Sanger, F., Nicklen, S. & Coulson, A. R.Proc. natn. Acad. Sci. U.S.A.74, 5463–5467 (1977).

    Article ADS CAS  Google Scholar 

  20. Melton, D. A.et al.Nucleic Acids Res.12, 7035–7056 (1984).

    Article CAS  Google Scholar 

  21. Krieg, P. A. & Melton, D. A.Nucleic Acids Res.12, 7057–7070 (1984).

    Article CAS  Google Scholar 

  22. Gasior, E., Herrera, F., Sadnik, I., McLaughlin, C. S. & Moldave, K.J. biol. Chem.254, 3965–3969 (1979).

    CAS PubMed  Google Scholar 

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Authors and Affiliations

  1. Tokyo Research Laboratory, Meito Sangyo Co. Ltd., 2973-2 Ishikawacho, Hachioji, Tokyo, 192, Japan

    Yoshiyuki Kawaguchi, Hiroshi Honda, Junko Taniguchi-Morimura & Shinjiro Iwasaki

Authors
  1. Yoshiyuki Kawaguchi

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  2. Hiroshi Honda

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  3. Junko Taniguchi-Morimura

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  4. Shinjiro Iwasaki

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Kawaguchi, Y., Honda, H., Taniguchi-Morimura, J.et al. The codon CUG is read as serine in an asporogenic yeastCandida cylindracea.Nature341, 164–166 (1989). https://doi.org/10.1038/341164a0

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