- Letter
- Published:
Light-dependent phosphorylation of rhodopsin byβ-adrenergic receptor kinase
Naturevolume 321, pages869–872 (1986)Cite this article
362Accesses
3Altmetric
Abstract
The structural components involved in transduction of extracellular signals as diverse as a photon of light impinging on the retina or a hormone molecule impinging on a cell have been highly conserved. These components include a recognition unit or receptor (for example, theβ-adrenergic receptor (βAR) for catecholamines or the ‘light receptor’ rhodopsin), a guanine nucleotide regulatory or transducing protein, and an effector enzyme (for example, adenylate cyclase or cyclic GMP phosphodiesterase)1,2. Molecular cloning has revealed that theβAR shares significant sequence and three-dimensional homology with rhodopsin3. The function of theβAR is diminished by exposure to stimulatory agonists, leading to desensitization4. Similarly, ‘light adaptation’ involves decreased coupling of photoactivated rhodopsin to cGMP phosphodiesterase activation5–7. Both forms of desensitization involve receptor phosphorylation. The latter is mediated by a unique protein kinase, rhodopsin kinase, which phosphorylates only the light-bleached form of rhodopsin8–10. An analogous enzyme (termedβAR kinase orβARK) phosphorylates only the agonist-occupiedβAR11. We report here thatβARK is also capable of phosphorylating rhodopsin in a totally light-dependent fashion. Moreover, rhodopsin kinase can phosphorylate the agonist-occupiedβAR. Thus the mechanisms which regulate the function of these disparate signalling systems also appear to be similar.
This is a preview of subscription content,access via your institution
Access options
Subscription info for Japanese customers
We have a dedicated website for our Japanese customers. Please go tonatureasia.com to subscribe to this journal.
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Fung, B. K.-K. & Stryer, L.Proc. natn. Acad. Sci. U.S.A.77, 2500–2504 (1980).
Lefkowitz, R. J., Stadel, J. M. & Caron, M. G.A. Rev. Biochem.52, 159–186 (1983).
Dixon, R. A. F.et al.Nature321, 75–79 (1986).
Sibley, D. R. & Lefkowitz, R. J.Nature317, 124–129 (1985).
Liebman, P. A. & Pugh, E. N.Nature287, 734–736 (1980).
Sitaramayya, A. & Liebman, P. A.J. boil. Chem.258, 1205–1209 (1983).
Wilden, U., Hall, S. W. & Kuhn, H.Proc. natn. Acad. Sci. U.S.A.83, 1174–1178 (1986).
Kuhn, H. & Dreyer, W. J.FEBS Lett.20, 1–6 (1972).
Bownds, D., Dawes, J., Miller, J. & Stahlman, M.Nature new Biol.237, 125–127 (1972).
Shichi, H. & Somers, R. L.J. biol. Chem.253, 7040–7046 (1978).
Benovic, J. L., Strasser, R. H., Caron, M. G. & Lefkowitz, R. J.Proc. natn. Acad. Sci. U.S.A.83, 2797–2081 (1986).
Paulsen, R., Miller, J. A., Brodie, A. E. & Bownds, M. D.Vision Res.15, 1325–1332 (1975).
Wilden, U. & Kuhn, H.Biochemistry21, 3014–3022 (1982).
Hargrave, P. A.et al.Neurochem. Int.1, 231–244 (1980).
Shichi, H., Yamamoto, K. & Somers, R. L.Vision Res.24, 1523–1531 (1984).
Leeb-Lundberg, L. M. F.et al.Proc. natn. Acad. Sci. U.S.A.82, 5651–5655 (1985).
Laemmli, U. K.Nature227, 680–685 (1970).
Benovic, J. L., Shorr, R. G. L., Caron, M. G. & Lefkowitz, R. J.Biochemistry23, 4510–4518 (1984).
Cerione, R. A., Strulovici, B., Benovic, J. L., Lefkowitz, R. J. & Caron, M. G.Nature306, 562–566 (1983).
Ovchinnikov, Y. A.FEBS Lett.148, 179–191 (1982).
Author information
Authors and Affiliations
Howard Hughes Medical Institute, Departments of Medicine, Biochemistry and Physiology, Duke University Medical Center, Durham, North Carolina, 27710, USA
Jeffrey L. Benovic, Federico Mayor Jr, Marc G. Caron & Robert J. Lefkowitz
National Eye Institute, National Institutes of Health, Bethesda, Maryland, 20892, USA
Robert L. Somers
- Jeffrey L. Benovic
You can also search for this author inPubMed Google Scholar
- Federico Mayor Jr
You can also search for this author inPubMed Google Scholar
- Robert L. Somers
You can also search for this author inPubMed Google Scholar
- Marc G. Caron
You can also search for this author inPubMed Google Scholar
- Robert J. Lefkowitz
You can also search for this author inPubMed Google Scholar
Rights and permissions
About this article
Cite this article
Benovic, J., Mayor, F., Somers, R.et al. Light-dependent phosphorylation of rhodopsin byβ-adrenergic receptor kinase.Nature321, 869–872 (1986). https://doi.org/10.1038/321869a0
Received:
Accepted:
Issue Date:
Share this article
Anyone you share the following link with will be able to read this content:
Sorry, a shareable link is not currently available for this article.
Provided by the Springer Nature SharedIt content-sharing initiative
